Stenotrophomonas maltophilia isolated from gasoline-contaminated soil is capable of degrading methyl tert-butyl ether

Background: Methyl tert-butyl ether (MTBE) is a pollutant that causes deleterious effects on human and environmental health. Certain microbial cultures have shown the ability to degrade MTBE, suggesting that a novel bacterial species capable of degrading MTBE could be recovered. The goal of this stu...

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Autores principales: Alfonso-Gordillo,Guadalupe, Cristiani-Urbina,Eliseo, Flores-Ortiz,César Mateo, Peralta,Humberto, Cancino-Díaz,Juan Carlos, Cruz-Maya,Juan Antonio, Jan-Roblero,Janet
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2016
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500003
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spelling oai:scielo:S0717-345820160005000032016-10-26Stenotrophomonas maltophilia isolated from gasoline-contaminated soil is capable of degrading methyl tert-butyl etherAlfonso-Gordillo,GuadalupeCristiani-Urbina,EliseoFlores-Ortiz,César MateoPeralta,HumbertoCancino-Díaz,Juan CarlosCruz-Maya,Juan AntonioJan-Roblero,Janet Bacterial consortium Biodegradation MTBE Sphingopyxis sp Stenotrophomonas maltophilia Background: Methyl tert-butyl ether (MTBE) is a pollutant that causes deleterious effects on human and environmental health. Certain microbial cultures have shown the ability to degrade MTBE, suggesting that a novel bacterial species capable of degrading MTBE could be recovered. The goal of this study was to isolate, identify and characterize the members of a bacterial consortium capable of degrading MTBE. Results: The IPN-120526 bacterial consortium was obtained through batch enrichment using MTBE as the sole carbon and energy source. The cultivable fraction of the consortium was identified; of the isolates, only Stenotrophomonas maltophilia IPN-TD and Sphingopyxis sp. IPN-TE were capable of degrading MTBE. To the best of our knowledge, this report is the first demonstrating that S. maltophilia and Sphingopyxis sp. are capable of degrading MTBE. The degradation kinetics of MTBE demonstrated that S. maltophilia IPN-TD had a significantly higher overall MTBE degradation efficiency and rate (48.39 ± 3.18% and 1.56 ± 0.12 mg L-1 h-1, respectively) than the IPN-120526 consortium (38.59 ± 2.17% and 1.25 ± 0.087 mg L-1 respectively). The kinetics of MTBE removal by both cultures fit first-order and pseudo-first-order reaction models. Conclusions: These findings suggest that S. maltophilia IPN-TD in axenic culture has considerable potential for the detoxification of MTBE-contaminated water.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.5 20162016-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500003en10.1016/j.ejbt.2016.06.006
institution Scielo Chile
collection Scielo Chile
language English
topic Bacterial consortium
Biodegradation
MTBE
Sphingopyxis sp
Stenotrophomonas maltophilia
spellingShingle Bacterial consortium
Biodegradation
MTBE
Sphingopyxis sp
Stenotrophomonas maltophilia
Alfonso-Gordillo,Guadalupe
Cristiani-Urbina,Eliseo
Flores-Ortiz,César Mateo
Peralta,Humberto
Cancino-Díaz,Juan Carlos
Cruz-Maya,Juan Antonio
Jan-Roblero,Janet
Stenotrophomonas maltophilia isolated from gasoline-contaminated soil is capable of degrading methyl tert-butyl ether
description Background: Methyl tert-butyl ether (MTBE) is a pollutant that causes deleterious effects on human and environmental health. Certain microbial cultures have shown the ability to degrade MTBE, suggesting that a novel bacterial species capable of degrading MTBE could be recovered. The goal of this study was to isolate, identify and characterize the members of a bacterial consortium capable of degrading MTBE. Results: The IPN-120526 bacterial consortium was obtained through batch enrichment using MTBE as the sole carbon and energy source. The cultivable fraction of the consortium was identified; of the isolates, only Stenotrophomonas maltophilia IPN-TD and Sphingopyxis sp. IPN-TE were capable of degrading MTBE. To the best of our knowledge, this report is the first demonstrating that S. maltophilia and Sphingopyxis sp. are capable of degrading MTBE. The degradation kinetics of MTBE demonstrated that S. maltophilia IPN-TD had a significantly higher overall MTBE degradation efficiency and rate (48.39 ± 3.18% and 1.56 ± 0.12 mg L-1 h-1, respectively) than the IPN-120526 consortium (38.59 ± 2.17% and 1.25 ± 0.087 mg L-1 respectively). The kinetics of MTBE removal by both cultures fit first-order and pseudo-first-order reaction models. Conclusions: These findings suggest that S. maltophilia IPN-TD in axenic culture has considerable potential for the detoxification of MTBE-contaminated water.
author Alfonso-Gordillo,Guadalupe
Cristiani-Urbina,Eliseo
Flores-Ortiz,César Mateo
Peralta,Humberto
Cancino-Díaz,Juan Carlos
Cruz-Maya,Juan Antonio
Jan-Roblero,Janet
author_facet Alfonso-Gordillo,Guadalupe
Cristiani-Urbina,Eliseo
Flores-Ortiz,César Mateo
Peralta,Humberto
Cancino-Díaz,Juan Carlos
Cruz-Maya,Juan Antonio
Jan-Roblero,Janet
author_sort Alfonso-Gordillo,Guadalupe
title Stenotrophomonas maltophilia isolated from gasoline-contaminated soil is capable of degrading methyl tert-butyl ether
title_short Stenotrophomonas maltophilia isolated from gasoline-contaminated soil is capable of degrading methyl tert-butyl ether
title_full Stenotrophomonas maltophilia isolated from gasoline-contaminated soil is capable of degrading methyl tert-butyl ether
title_fullStr Stenotrophomonas maltophilia isolated from gasoline-contaminated soil is capable of degrading methyl tert-butyl ether
title_full_unstemmed Stenotrophomonas maltophilia isolated from gasoline-contaminated soil is capable of degrading methyl tert-butyl ether
title_sort stenotrophomonas maltophilia isolated from gasoline-contaminated soil is capable of degrading methyl tert-butyl ether
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2016
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500003
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