Identification and genetic diversity analysis of Memecylon species using ISSR, RAPD and Gene-Based DNA barcoding tools

Background: Memecylon species are commonly used in Indian ethnomedical practices. The accurate identification is vital to enhance the drug's efficacy and biosafety. In the present study, PCR based techniques like RAPD, ISSR and DNA barcoding regions, such as 5s, psbA-trnH, rpoC1, ndh and atpF-a...

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Autores principales:	Tumkur Ramasetty,Bharathi, Naik Bajpe,Shrisha, Kigga Kadappa,Sampath Kumara, Kumar Saini,Ramesh, Nittur Basavaraju,Shashibhushan, Kukkundoor Ramachandra,Kini, Harishchandra Sripathy,Prakash
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2016
Materias:	atpF-atpH Memecylon species ndh psbA-trnH rpoC1 5s PCR based techniques Molecular phylogenetic analysis Molecular phylogenesis DNA markers
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600001
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spelling	oai:scielo:S0717-345820160006000012017-01-25Identification and genetic diversity analysis of Memecylon species using ISSR, RAPD and Gene-Based DNA barcoding toolsTumkur Ramasetty,BharathiNaik Bajpe,ShrishaKigga Kadappa,Sampath KumaraKumar Saini,RameshNittur Basavaraju,ShashibhushanKukkundoor Ramachandra,KiniHarishchandra Sripathy,Prakash atpF-atpH Memecylon species ndh psbA-trnH rpoC1 5s PCR based techniques Molecular phylogenetic analysis Molecular phylogenesis DNA markers Background: Memecylon species are commonly used in Indian ethnomedical practices. The accurate identification is vital to enhance the drug's efficacy and biosafety. In the present study, PCR based techniques like RAPD, ISSR and DNA barcoding regions, such as 5s, psbA-trnH, rpoC1, ndh and atpF-atpH, were used to authenticate and analyze the diversity of five Memecylon species collected from Western Ghats of India. Results: Phylogenetic analysis clearly distinguished Memecylon malabaricum from Memecylon wightii and Memecylon umbellatum from Memecylon edule and clades formed are in accordance with morphological keys. In the RAPD and ISSR analyses, 27 accessions representing five Memecylon species were distinctly separated into three different clades. M. malabaricum and M. wightii grouped together and M. umbellatum, M. edule and Memecylon talbotianum grouped in the same clade with high Jaccard dissimilarity coefficient and bootstrap support between each node, indicating that these grouped species are phylogenetically similar. Conclusion: Data from the present study reveals that chloroplast psbA-trnH region could be used as a potential candidate region for identifying Memecylon species, and ISSR marker system could be used for estimating genetic diversity since it has high percent polymorphism compared to RAPD marker.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.6 20162016-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600001en10.1016/j.ejbt.2016.09.001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	atpF-atpH Memecylon species ndh psbA-trnH rpoC1 5s PCR based techniques Molecular phylogenetic analysis Molecular phylogenesis DNA markers
spellingShingle	atpF-atpH Memecylon species ndh psbA-trnH rpoC1 5s PCR based techniques Molecular phylogenetic analysis Molecular phylogenesis DNA markers Tumkur Ramasetty,Bharathi Naik Bajpe,Shrisha Kigga Kadappa,Sampath Kumara Kumar Saini,Ramesh Nittur Basavaraju,Shashibhushan Kukkundoor Ramachandra,Kini Harishchandra Sripathy,Prakash Identification and genetic diversity analysis of Memecylon species using ISSR, RAPD and Gene-Based DNA barcoding tools
description	Background: Memecylon species are commonly used in Indian ethnomedical practices. The accurate identification is vital to enhance the drug's efficacy and biosafety. In the present study, PCR based techniques like RAPD, ISSR and DNA barcoding regions, such as 5s, psbA-trnH, rpoC1, ndh and atpF-atpH, were used to authenticate and analyze the diversity of five Memecylon species collected from Western Ghats of India. Results: Phylogenetic analysis clearly distinguished Memecylon malabaricum from Memecylon wightii and Memecylon umbellatum from Memecylon edule and clades formed are in accordance with morphological keys. In the RAPD and ISSR analyses, 27 accessions representing five Memecylon species were distinctly separated into three different clades. M. malabaricum and M. wightii grouped together and M. umbellatum, M. edule and Memecylon talbotianum grouped in the same clade with high Jaccard dissimilarity coefficient and bootstrap support between each node, indicating that these grouped species are phylogenetically similar. Conclusion: Data from the present study reveals that chloroplast psbA-trnH region could be used as a potential candidate region for identifying Memecylon species, and ISSR marker system could be used for estimating genetic diversity since it has high percent polymorphism compared to RAPD marker.
author	Tumkur Ramasetty,Bharathi Naik Bajpe,Shrisha Kigga Kadappa,Sampath Kumara Kumar Saini,Ramesh Nittur Basavaraju,Shashibhushan Kukkundoor Ramachandra,Kini Harishchandra Sripathy,Prakash
author_facet	Tumkur Ramasetty,Bharathi Naik Bajpe,Shrisha Kigga Kadappa,Sampath Kumara Kumar Saini,Ramesh Nittur Basavaraju,Shashibhushan Kukkundoor Ramachandra,Kini Harishchandra Sripathy,Prakash
author_sort	Tumkur Ramasetty,Bharathi
title	Identification and genetic diversity analysis of Memecylon species using ISSR, RAPD and Gene-Based DNA barcoding tools
title_short	Identification and genetic diversity analysis of Memecylon species using ISSR, RAPD and Gene-Based DNA barcoding tools
title_full	Identification and genetic diversity analysis of Memecylon species using ISSR, RAPD and Gene-Based DNA barcoding tools
title_fullStr	Identification and genetic diversity analysis of Memecylon species using ISSR, RAPD and Gene-Based DNA barcoding tools
title_full_unstemmed	Identification and genetic diversity analysis of Memecylon species using ISSR, RAPD and Gene-Based DNA barcoding tools
title_sort	identification and genetic diversity analysis of memecylon species using issr, rapd and gene-based dna barcoding tools
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600001
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Identification and genetic diversity analysis of Memecylon species using ISSR, RAPD and Gene-Based DNA barcoding tools

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