Strategy of oxygen transfer coefficient control on the L-erythrulose fermentation by newly isolated Gluconobacter kondonii

Background: The effect of diverse oxygen transfer coefficient on the L-erythrulose production from meso-erythritol by a newly isolated strain, Gluconobacter kondonii CGMCC8391 was investigated. In order to elucidate the effects of volumetric mass transfer coefficient (K La) on the fermentations, baf...

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Autores principales:	Pan,Long, Fang,Ya-kun, Zhou,Pei, Jin,Kui-qi, Gang,Wang, Liu,Yu-peng
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2016
Materias:	K La Industrial biotechnology Process control Production Tanning agents Tanning properties Artificial tanning Dihydroxypropanone (DHA) Production of L-erythrulose Meso-erythritol Biosynthesis of L-erythrulose
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600004
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spelling	oai:scielo:S0717-345820160006000042017-01-25Strategy of oxygen transfer coefficient control on the L-erythrulose fermentation by newly isolated Gluconobacter kondoniiPan,LongFang,Ya-kunZhou,PeiJin,Kui-qiGang,WangLiu,Yu-peng K La Industrial biotechnology Process control Production Tanning agents Tanning properties Artificial tanning Dihydroxypropanone (DHA) Production of L-erythrulose Meso-erythritol Biosynthesis of L-erythrulose Background: The effect of diverse oxygen transfer coefficient on the L-erythrulose production from meso-erythritol by a newly isolated strain, Gluconobacter kondonii CGMCC8391 was investigated. In order to elucidate the effects of volumetric mass transfer coefficient (K La) on the fermentations, baffled and unbaffled flask cultures, and fed-batch cultures were developed in present work. Results: With the increase of the K La value in the fed-batch culture, L-erythrulose concentration, productivity and yield were significantly improved, while cell growth was not the best in the high K La. Thus, a two-stage oxygen supply control strategy was proposed, aimed at achieving high concentration and high productivity of L-erythrulose. During the first 12 h, Klawas controlled at 40.28 h-1 to obtain high value for cell growth, subsequently K La was controlled at 86.31 h-1 to allow for high L-erythrulose accumulation. Conclusions: Under optimal conditions, the L-erythrulose concentration, productivity, yield and DCW reached 207.9 ± 7.78 g/L, 6.50 g/L/h, 0.94 g/g, 2.68 ± 0.17 g/L, respectively. At the end of fermentation, the L-erythrulose concentration and productivity were higher than those in the previous similar reports.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.6 20162016-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600004en10.1016/j.ejbt.2016.08.006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	K La Industrial biotechnology Process control Production Tanning agents Tanning properties Artificial tanning Dihydroxypropanone (DHA) Production of L-erythrulose Meso-erythritol Biosynthesis of L-erythrulose
spellingShingle	K La Industrial biotechnology Process control Production Tanning agents Tanning properties Artificial tanning Dihydroxypropanone (DHA) Production of L-erythrulose Meso-erythritol Biosynthesis of L-erythrulose Pan,Long Fang,Ya-kun Zhou,Pei Jin,Kui-qi Gang,Wang Liu,Yu-peng Strategy of oxygen transfer coefficient control on the L-erythrulose fermentation by newly isolated Gluconobacter kondonii
description	Background: The effect of diverse oxygen transfer coefficient on the L-erythrulose production from meso-erythritol by a newly isolated strain, Gluconobacter kondonii CGMCC8391 was investigated. In order to elucidate the effects of volumetric mass transfer coefficient (K La) on the fermentations, baffled and unbaffled flask cultures, and fed-batch cultures were developed in present work. Results: With the increase of the K La value in the fed-batch culture, L-erythrulose concentration, productivity and yield were significantly improved, while cell growth was not the best in the high K La. Thus, a two-stage oxygen supply control strategy was proposed, aimed at achieving high concentration and high productivity of L-erythrulose. During the first 12 h, Klawas controlled at 40.28 h-1 to obtain high value for cell growth, subsequently K La was controlled at 86.31 h-1 to allow for high L-erythrulose accumulation. Conclusions: Under optimal conditions, the L-erythrulose concentration, productivity, yield and DCW reached 207.9 ± 7.78 g/L, 6.50 g/L/h, 0.94 g/g, 2.68 ± 0.17 g/L, respectively. At the end of fermentation, the L-erythrulose concentration and productivity were higher than those in the previous similar reports.
author	Pan,Long Fang,Ya-kun Zhou,Pei Jin,Kui-qi Gang,Wang Liu,Yu-peng
author_facet	Pan,Long Fang,Ya-kun Zhou,Pei Jin,Kui-qi Gang,Wang Liu,Yu-peng
author_sort	Pan,Long
title	Strategy of oxygen transfer coefficient control on the L-erythrulose fermentation by newly isolated Gluconobacter kondonii
title_short	Strategy of oxygen transfer coefficient control on the L-erythrulose fermentation by newly isolated Gluconobacter kondonii
title_full	Strategy of oxygen transfer coefficient control on the L-erythrulose fermentation by newly isolated Gluconobacter kondonii
title_fullStr	Strategy of oxygen transfer coefficient control on the L-erythrulose fermentation by newly isolated Gluconobacter kondonii
title_full_unstemmed	Strategy of oxygen transfer coefficient control on the L-erythrulose fermentation by newly isolated Gluconobacter kondonii
title_sort	strategy of oxygen transfer coefficient control on the l-erythrulose fermentation by newly isolated gluconobacter kondonii
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600004
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_version_	1718441937150672896

Strategy of oxygen transfer coefficient control on the L-erythrulose fermentation by newly isolated Gluconobacter kondonii

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