Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins

Background: Synthesized aminocoumarins are heterocyclic compounds possessing potential for the treatment of insulin-dependent diabetes mellitus with unexplored anti-glycative action. Results: In this study 4-aminocoumarin derivatives (4-ACDs) were evaluated in vitro for antiglycation (AG) activities...

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Autores principales:	Aminjafari,Akram, Miroliaei,Mehran, Angelova,Violina T, Emamzadeh,Rahman, Djukic,Mirjana M, Djuric,Ana, Saso,Luciano
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2016
Materias:	Anti-glycation agents Diabetes, 4-Aminocoumarin derivatives Congo red Spectropolarimetry Spectrofluorimetry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600007
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spelling	oai:scielo:S0717-345820160006000072017-01-25Antioxidant activity and protective role on protein glycation of synthetic aminocoumarinsAminjafari,AkramMiroliaei,MehranAngelova,Violina TEmamzadeh,RahmanDjukic,Mirjana MDjuric,AnaSaso,Luciano Anti-glycation agents Diabetes, 4-Aminocoumarin derivatives Congo red Spectropolarimetry Spectrofluorimetry Background: Synthesized aminocoumarins are heterocyclic compounds possessing potential for the treatment of insulin-dependent diabetes mellitus with unexplored anti-glycative action. Results: In this study 4-aminocoumarin derivatives (4-ACDs) were evaluated in vitro for antiglycation (AG) activities by using the human serum albumin (HSA)/glucose system, for 8 weeks of incubation. The glycation and conformational alteration of HSA in the presence of the tested compounds were evaluated by Congo red assay, fluorescence and circular dichroism spectroscopy. The antioxidant (AO) capacity were also tested by four different assays including: DPPH (2,2'-diphenyl-1-picrylhydrazyl radical), ABTS (2,2-azinobis (3-ethylbenzothiazoline-6-sulphonate) diammonium salt), FRAP (ferric reducing antioxidant power) and β-carotene-linoleic acid assay. The tested compounds showed AG and AO effects. The intensity of the accomplished AO potential is related to the type of the used assay. Significant alterations in the secondary (monitored by CD spectropolarimetry) and tertiary structure (assessed by spectrofluorimetry) of HSA upon glycation were mitigated by the 4-ACDs, suggesting their suppressive role in the late stage (post-Amadori) of the HSA glycation. Conclusions: By the analogues, in vitro ascertained AO and AG properties of 4-ACD may be recognized as rationale for their protective role against oxidative changes of proteins, thereby precluding diabetic complications in humans.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.6 20162016-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600007en10.1016/j.ejbt.2016.08.004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Anti-glycation agents Diabetes, 4-Aminocoumarin derivatives Congo red Spectropolarimetry Spectrofluorimetry
spellingShingle	Anti-glycation agents Diabetes, 4-Aminocoumarin derivatives Congo red Spectropolarimetry Spectrofluorimetry Aminjafari,Akram Miroliaei,Mehran Angelova,Violina T Emamzadeh,Rahman Djukic,Mirjana M Djuric,Ana Saso,Luciano Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins
description	Background: Synthesized aminocoumarins are heterocyclic compounds possessing potential for the treatment of insulin-dependent diabetes mellitus with unexplored anti-glycative action. Results: In this study 4-aminocoumarin derivatives (4-ACDs) were evaluated in vitro for antiglycation (AG) activities by using the human serum albumin (HSA)/glucose system, for 8 weeks of incubation. The glycation and conformational alteration of HSA in the presence of the tested compounds were evaluated by Congo red assay, fluorescence and circular dichroism spectroscopy. The antioxidant (AO) capacity were also tested by four different assays including: DPPH (2,2'-diphenyl-1-picrylhydrazyl radical), ABTS (2,2-azinobis (3-ethylbenzothiazoline-6-sulphonate) diammonium salt), FRAP (ferric reducing antioxidant power) and β-carotene-linoleic acid assay. The tested compounds showed AG and AO effects. The intensity of the accomplished AO potential is related to the type of the used assay. Significant alterations in the secondary (monitored by CD spectropolarimetry) and tertiary structure (assessed by spectrofluorimetry) of HSA upon glycation were mitigated by the 4-ACDs, suggesting their suppressive role in the late stage (post-Amadori) of the HSA glycation. Conclusions: By the analogues, in vitro ascertained AO and AG properties of 4-ACD may be recognized as rationale for their protective role against oxidative changes of proteins, thereby precluding diabetic complications in humans.
author	Aminjafari,Akram Miroliaei,Mehran Angelova,Violina T Emamzadeh,Rahman Djukic,Mirjana M Djuric,Ana Saso,Luciano
author_facet	Aminjafari,Akram Miroliaei,Mehran Angelova,Violina T Emamzadeh,Rahman Djukic,Mirjana M Djuric,Ana Saso,Luciano
author_sort	Aminjafari,Akram
title	Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins
title_short	Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins
title_full	Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins
title_fullStr	Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins
title_full_unstemmed	Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins
title_sort	antioxidant activity and protective role on protein glycation of synthetic aminocoumarins
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600007
work_keys_str_mv	AT aminjafariakram antioxidantactivityandprotectiveroleonproteinglycationofsyntheticaminocoumarins AT miroliaeimehran antioxidantactivityandprotectiveroleonproteinglycationofsyntheticaminocoumarins AT angelovaviolinat antioxidantactivityandprotectiveroleonproteinglycationofsyntheticaminocoumarins AT emamzadehrahman antioxidantactivityandprotectiveroleonproteinglycationofsyntheticaminocoumarins AT djukicmirjanam antioxidantactivityandprotectiveroleonproteinglycationofsyntheticaminocoumarins AT djuricana antioxidantactivityandprotectiveroleonproteinglycationofsyntheticaminocoumarins AT sasoluciano antioxidantactivityandprotectiveroleonproteinglycationofsyntheticaminocoumarins
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Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins

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