Expression analysis of vasa in Asian paddle crab (Charybdis japónica) exposed to Bisphenol A

Background: Bisphenol A (BPA) is an endocrine-disrupting chemical (EDC) with a weak estrogen-like activity in fish that is found ubiquitously in aquatic environments. However, there has been little study about BPA on the endocrine disrupting effects of crab. In the present study, cDNA of vasa was cl...

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Autores principales:	Chen,Jianhua, Wang,Cuihua, Gao,Huan, Yan,Binlun
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2016
Materias:	Aquatic environments BPA DEAD-box Endocrine disrupting Expression analysis Expression patterns Histological observation vasa vasa cDNA vasa gene vasa mRNA
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600008
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spelling	oai:scielo:S0717-345820160006000082017-01-25Expression analysis of vasa in Asian paddle crab (Charybdis japónica) exposed to Bisphenol AChen,JianhuaWang,CuihuaGao,HuanYan,Binlun Aquatic environments BPA DEAD-box Endocrine disrupting Expression analysis Expression patterns Histological observation vasa vasa cDNA vasa gene vasa mRNA Background: Bisphenol A (BPA) is an endocrine-disrupting chemical (EDC) with a weak estrogen-like activity in fish that is found ubiquitously in aquatic environments. However, there has been little study about BPA on the endocrine disrupting effects of crab. In the present study, cDNA of vasa was cloned and characterized in the Charybdis japonica. Histological structures of testis and expression patterns of vasa gene in the testis of C. japonica after treatment with BPA were investigated. Results: The cDNA of vasa is composed of 3051 bp with a 2166 bp open reading frame encoding 721 AA. The deduced amino acid sequence contained eight conserved domains of the DEAD-box protein family. The tissue distribution showed that vasa mRNA was specifically expressed in ovary and testis. Histologically, the sperm cells were decreased in number and an acellular zone was seen in the testis. The transcript level of vasa gradually increased with a significant difference between the experimental and control groups. After BPA exposure with 0.50 and 1.00 mg/L for 1,3, 6 and 9 d, the expression levels of vasa increased. Conclusion: These findings suggest that BPA can increase the expression level of vasa mRNA and influence the development of the testis in C. japonica.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.6 20162016-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600008en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aquatic environments BPA DEAD-box Endocrine disrupting Expression analysis Expression patterns Histological observation vasa vasa cDNA vasa gene vasa mRNA
spellingShingle	Aquatic environments BPA DEAD-box Endocrine disrupting Expression analysis Expression patterns Histological observation vasa vasa cDNA vasa gene vasa mRNA Chen,Jianhua Wang,Cuihua Gao,Huan Yan,Binlun Expression analysis of vasa in Asian paddle crab (Charybdis japónica) exposed to Bisphenol A
description	Background: Bisphenol A (BPA) is an endocrine-disrupting chemical (EDC) with a weak estrogen-like activity in fish that is found ubiquitously in aquatic environments. However, there has been little study about BPA on the endocrine disrupting effects of crab. In the present study, cDNA of vasa was cloned and characterized in the Charybdis japonica. Histological structures of testis and expression patterns of vasa gene in the testis of C. japonica after treatment with BPA were investigated. Results: The cDNA of vasa is composed of 3051 bp with a 2166 bp open reading frame encoding 721 AA. The deduced amino acid sequence contained eight conserved domains of the DEAD-box protein family. The tissue distribution showed that vasa mRNA was specifically expressed in ovary and testis. Histologically, the sperm cells were decreased in number and an acellular zone was seen in the testis. The transcript level of vasa gradually increased with a significant difference between the experimental and control groups. After BPA exposure with 0.50 and 1.00 mg/L for 1,3, 6 and 9 d, the expression levels of vasa increased. Conclusion: These findings suggest that BPA can increase the expression level of vasa mRNA and influence the development of the testis in C. japonica.
author	Chen,Jianhua Wang,Cuihua Gao,Huan Yan,Binlun
author_facet	Chen,Jianhua Wang,Cuihua Gao,Huan Yan,Binlun
author_sort	Chen,Jianhua
title	Expression analysis of vasa in Asian paddle crab (Charybdis japónica) exposed to Bisphenol A
title_short	Expression analysis of vasa in Asian paddle crab (Charybdis japónica) exposed to Bisphenol A
title_full	Expression analysis of vasa in Asian paddle crab (Charybdis japónica) exposed to Bisphenol A
title_fullStr	Expression analysis of vasa in Asian paddle crab (Charybdis japónica) exposed to Bisphenol A
title_full_unstemmed	Expression analysis of vasa in Asian paddle crab (Charybdis japónica) exposed to Bisphenol A
title_sort	expression analysis of vasa in asian paddle crab (charybdis japónica) exposed to bisphenol a
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600008
work_keys_str_mv	AT chenjianhua expressionanalysisofvasainasianpaddlecrabcharybdisjaponicaexposedtobisphenola AT wangcuihua expressionanalysisofvasainasianpaddlecrabcharybdisjaponicaexposedtobisphenola AT gaohuan expressionanalysisofvasainasianpaddlecrabcharybdisjaponicaexposedtobisphenola AT yanbinlun expressionanalysisofvasainasianpaddlecrabcharybdisjaponicaexposedtobisphenola
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Expression analysis of vasa in Asian paddle crab (Charybdis japónica) exposed to Bisphenol A

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