Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.)

Background: Marker-assisted introgression currently represents the most widely spread application of DNA markers as an aid to selection in plant breeding. New barley germplasm should be supplemented by genes that facilitate growth and development under stressful conditions. The homology search again...

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Autores principales:	Mikolajczak,Krzysztof, Ogrodowicz,Piotr, Surma,Maria, Adamski,Tadeusz, Kuczyñska,Anetta
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2016
Materias:	BC lines Gene annotation Semi-dwarf gene Single nucleotide polymorphism spring barley Marker selection Marker-assisted backcrossing Marker-assisted introgression DNA markers Selection in plant breeding
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600014
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spelling	oai:scielo:S0717-345820160006000142017-01-25Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.)Mikolajczak,KrzysztofOgrodowicz,PiotrSurma,MariaAdamski,TadeuszKuczyñska,Anetta BC lines Gene annotation Semi-dwarf gene Single nucleotide polymorphism spring barley Marker selection Marker-assisted backcrossing Marker-assisted introgression DNA markers Selection in plant breeding Background: Marker-assisted introgression currently represents the most widely spread application of DNA markers as an aid to selection in plant breeding. New barley germplasm should be supplemented by genes that facilitate growth and development under stressful conditions. The homology search against known genes is a fundamental approach to identify genes among the generated sequences. This procedure can be utilized for SNP search in genes of predicted function of interest and associated gene ontology (GO). Results: Backcross breeding enhanced by marker selection may become a powerful method to transfer one or a few genes controlling a specific trait. In the study, the integrated approach of combining phenotypic selection with marker assisted backcross breeding for introgression of LTP2 gene, in the background of semi-dwarf spring barley cultivar, was employed. This study discusses the efficiency of molecular marker application in backcrossing targeted on the selected gene. Conclusions: BC6 lines developed in this study can serve as a unique and adequate plant material to dissect the role of LTP2 gene. Due to its role in lipid transfer, the LTP2 may be crucial in lipidome modification in response to abiotic stress.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.6 20162016-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600014en10.1016/j.ejbt.2016.09.003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	BC lines Gene annotation Semi-dwarf gene Single nucleotide polymorphism spring barley Marker selection Marker-assisted backcrossing Marker-assisted introgression DNA markers Selection in plant breeding
spellingShingle	BC lines Gene annotation Semi-dwarf gene Single nucleotide polymorphism spring barley Marker selection Marker-assisted backcrossing Marker-assisted introgression DNA markers Selection in plant breeding Mikolajczak,Krzysztof Ogrodowicz,Piotr Surma,Maria Adamski,Tadeusz Kuczyñska,Anetta Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.)
description	Background: Marker-assisted introgression currently represents the most widely spread application of DNA markers as an aid to selection in plant breeding. New barley germplasm should be supplemented by genes that facilitate growth and development under stressful conditions. The homology search against known genes is a fundamental approach to identify genes among the generated sequences. This procedure can be utilized for SNP search in genes of predicted function of interest and associated gene ontology (GO). Results: Backcross breeding enhanced by marker selection may become a powerful method to transfer one or a few genes controlling a specific trait. In the study, the integrated approach of combining phenotypic selection with marker assisted backcross breeding for introgression of LTP2 gene, in the background of semi-dwarf spring barley cultivar, was employed. This study discusses the efficiency of molecular marker application in backcrossing targeted on the selected gene. Conclusions: BC6 lines developed in this study can serve as a unique and adequate plant material to dissect the role of LTP2 gene. Due to its role in lipid transfer, the LTP2 may be crucial in lipidome modification in response to abiotic stress.
author	Mikolajczak,Krzysztof Ogrodowicz,Piotr Surma,Maria Adamski,Tadeusz Kuczyñska,Anetta
author_facet	Mikolajczak,Krzysztof Ogrodowicz,Piotr Surma,Maria Adamski,Tadeusz Kuczyñska,Anetta
author_sort	Mikolajczak,Krzysztof
title	Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.)
title_short	Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.)
title_full	Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.)
title_fullStr	Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.)
title_full_unstemmed	Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.)
title_sort	introgression of ltp2 gene through marker assisted backcross in barley (hordeum vulgare l.)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600014
work_keys_str_mv	AT mikolajczakkrzysztof introgressionofltp2genethroughmarkerassistedbackcrossinbarleyhordeumvulgarel AT ogrodowiczpiotr introgressionofltp2genethroughmarkerassistedbackcrossinbarleyhordeumvulgarel AT surmamaria introgressionofltp2genethroughmarkerassistedbackcrossinbarleyhordeumvulgarel AT adamskitadeusz introgressionofltp2genethroughmarkerassistedbackcrossinbarleyhordeumvulgarel AT kuczynskaanetta introgressionofltp2genethroughmarkerassistedbackcrossinbarleyhordeumvulgarel
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Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.)

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