AUTHENTICATION OF COMMERCIAL CHILEAN MOLLUSKS USING RIBOSOMAL INTERNAL TRANSCRIBED SPACER (ITS) AS SPECIE-SPECIFIC DNA MARKER

Contemporary world food safety standards have emphasized the implementation of efficient food traceability systems, including the correct labeling of products for the authentication of the commercialized species. In this context, Chile has reached an important development in agricultural exportation...

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Autores principales:	Aguilera-Muñoz,Felipe, Valenzuela-Muñoz,Valentina, Gallardo-Escárate,Cristian
Lenguaje:	English
Publicado:	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción 2008
Materias:	Genetic traceability ITS 1 ITS2 mo Husk identification specific divider design
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382008000200007
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spelling	oai:scielo:S0717-653820080002000072009-06-16AUTHENTICATION OF COMMERCIAL CHILEAN MOLLUSKS USING RIBOSOMAL INTERNAL TRANSCRIBED SPACER (ITS) AS SPECIE-SPECIFIC DNA MARKERAguilera-Muñoz,FelipeValenzuela-Muñoz,ValentinaGallardo-Escárate,Cristian Genetic traceability ITS 1 ITS2 mo Husk identification specific divider design Contemporary world food safety standards have emphasized the implementation of efficient food traceability systems, including the correct labeling of products for the authentication of the commercialized species. In this context, Chile has reached an important development in agricultural exportations, reaching near US$ 3.82 billion in 2007. Since the identification based on morphologic and organoleptic characteristics is complex during the elaboration process of aquaculture products, molecular tools need to be developed in order to genetically trace aquaculture products. This study analyzed five species of Chilean commercial mo Husks using molecular DNA markers. From the partial sequences of the ribosomal region ITSl-5.8SrDNA-ITS2, the specific primers for the internal transcribed spacers 1 and 2 (ITS1, ITS2) were designed. These primers in 8 fresh species of mollusks, 5 commercial presentations and 3 processed species, obtaining specie-specific band patterns in all cases for both ITS. Additionally, the primers are shown to be highly specific and of replicable amplification products, independently from the commercial presentation of the studied species. Genetically trace aquaculture products. The presence of fraud for species substitution between canned abalone (Haliotis) and loco (Concholepas) species is discussed.info:eu-repo/semantics/openAccessFacultad de Ciencias Naturales y Oceanográficas, Universidad de ConcepciónGayana (Concepción) v.72 n.2 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382008000200007en10.4067/S0717-65382008000200007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Genetic traceability ITS 1 ITS2 mo Husk identification specific divider design
spellingShingle	Genetic traceability ITS 1 ITS2 mo Husk identification specific divider design Aguilera-Muñoz,Felipe Valenzuela-Muñoz,Valentina Gallardo-Escárate,Cristian AUTHENTICATION OF COMMERCIAL CHILEAN MOLLUSKS USING RIBOSOMAL INTERNAL TRANSCRIBED SPACER (ITS) AS SPECIE-SPECIFIC DNA MARKER
description	Contemporary world food safety standards have emphasized the implementation of efficient food traceability systems, including the correct labeling of products for the authentication of the commercialized species. In this context, Chile has reached an important development in agricultural exportations, reaching near US$ 3.82 billion in 2007. Since the identification based on morphologic and organoleptic characteristics is complex during the elaboration process of aquaculture products, molecular tools need to be developed in order to genetically trace aquaculture products. This study analyzed five species of Chilean commercial mo Husks using molecular DNA markers. From the partial sequences of the ribosomal region ITSl-5.8SrDNA-ITS2, the specific primers for the internal transcribed spacers 1 and 2 (ITS1, ITS2) were designed. These primers in 8 fresh species of mollusks, 5 commercial presentations and 3 processed species, obtaining specie-specific band patterns in all cases for both ITS. Additionally, the primers are shown to be highly specific and of replicable amplification products, independently from the commercial presentation of the studied species. Genetically trace aquaculture products. The presence of fraud for species substitution between canned abalone (Haliotis) and loco (Concholepas) species is discussed.
author	Aguilera-Muñoz,Felipe Valenzuela-Muñoz,Valentina Gallardo-Escárate,Cristian
author_facet	Aguilera-Muñoz,Felipe Valenzuela-Muñoz,Valentina Gallardo-Escárate,Cristian
author_sort	Aguilera-Muñoz,Felipe
title	AUTHENTICATION OF COMMERCIAL CHILEAN MOLLUSKS USING RIBOSOMAL INTERNAL TRANSCRIBED SPACER (ITS) AS SPECIE-SPECIFIC DNA MARKER
title_short	AUTHENTICATION OF COMMERCIAL CHILEAN MOLLUSKS USING RIBOSOMAL INTERNAL TRANSCRIBED SPACER (ITS) AS SPECIE-SPECIFIC DNA MARKER
title_full	AUTHENTICATION OF COMMERCIAL CHILEAN MOLLUSKS USING RIBOSOMAL INTERNAL TRANSCRIBED SPACER (ITS) AS SPECIE-SPECIFIC DNA MARKER
title_fullStr	AUTHENTICATION OF COMMERCIAL CHILEAN MOLLUSKS USING RIBOSOMAL INTERNAL TRANSCRIBED SPACER (ITS) AS SPECIE-SPECIFIC DNA MARKER
title_full_unstemmed	AUTHENTICATION OF COMMERCIAL CHILEAN MOLLUSKS USING RIBOSOMAL INTERNAL TRANSCRIBED SPACER (ITS) AS SPECIE-SPECIFIC DNA MARKER
title_sort	authentication of commercial chilean mollusks using ribosomal internal transcribed spacer (its) as specie-specific dna marker
publisher	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382008000200007
work_keys_str_mv	AT aguileramunozfelipe authenticationofcommercialchileanmollusksusingribosomalinternaltranscribedspaceritsasspeciespecificdnamarker AT valenzuelamunozvalentina authenticationofcommercialchileanmollusksusingribosomalinternaltranscribedspaceritsasspeciespecificdnamarker AT gallardoescaratecristian authenticationofcommercialchileanmollusksusingribosomalinternaltranscribedspaceritsasspeciespecificdnamarker
_version_	1718442126770962432

AUTHENTICATION OF COMMERCIAL CHILEAN MOLLUSKS USING RIBOSOMAL INTERNAL TRANSCRIBED SPACER (ITS) AS SPECIE-SPECIFIC DNA MARKER

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