ANALISIS MOLECULAR EN GASTROPODOS CHILENOS COMERCIALES BASADOS EN LAS SECUENCIAS 16S rRNA,. COI Y ITS1-5.8S rDNA-ITS2

Gastropod mollusks are part of the principal marine resources cultivated and commercialized in Chile. There are native Chilean species such as loco (Concholepas concholepas), locate (Thais chocolata), trumulco snail (Chorus giganteus), keyhole limpets (Fissurella spp.), tegula snail (Tegula atra) as...

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Autores principales:	Aguilera-Muñoz,Felipe, Lafarga-Cruz,Fabiola, Gallardo-Escárate,Cristian
Lenguaje:	English
Publicado:	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción 2009
Materias:	16S rRNA COI ITSl-5.8SrDNA-ITS2 gastropod mollusks phylogenetic relationships
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382009000100003
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spelling	oai:scielo:S0717-653820090001000032010-04-05ANALISIS MOLECULAR EN GASTROPODOS CHILENOS COMERCIALES BASADOS EN LAS SECUENCIAS 16S rRNA,. COI Y ITS1-5.8S rDNA-ITS2Aguilera-Muñoz,FelipeLafarga-Cruz,FabiolaGallardo-Escárate,Cristian 16S rRNA COI ITSl-5.8SrDNA-ITS2 gastropod mollusks phylogenetic relationships Gastropod mollusks are part of the principal marine resources cultivated and commercialized in Chile. There are native Chilean species such as loco (Concholepas concholepas), locate (Thais chocolata), trumulco snail (Chorus giganteus), keyhole limpets (Fissurella spp.), tegula snail (Tegula atra) as well as exotic species such as red abalone (Haliotis rufescens) and Japanese abalone (Haliotis discus hannai). Despite their importance as marine resources, molecular genetic studies establishing phylogenetic relationships and estimating population genetic parameters are scarce. The aim of this study is to establish a molecular approach among the main commercial gastropod species in Chile. The mitochondrial genes 16S rRNA and COI, and the nuclear ribosomal region ITSl-5.8SrDNA-ITS2 were amplified by PCR and sequencing. Alignment analysis was used to determine systematic relationships at the specific level for the species studied. The results revealed that 7 species are grouped in 4 genetically distinct families (Haliotidae, Trochidae, Muricidae and Fissurellidae). In comparison with COI sequencing, 16S rRNA and ITSl-5.8SrDNA-ITS2 sequencing were relatively more conserved with a divergence percentage for 16S rDNA and ITSl-5.8SrDNA-ITS2 of 1.2% and 1.8%, respectively, contrasting with the value of 10% obtained for COI in abalone.info:eu-repo/semantics/openAccessFacultad de Ciencias Naturales y Oceanográficas, Universidad de ConcepciónGayana (Concepción) v.73 n.1 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382009000100003en10.4067/S0717-65382009000100003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	16S rRNA COI ITSl-5.8SrDNA-ITS2 gastropod mollusks phylogenetic relationships
spellingShingle	16S rRNA COI ITSl-5.8SrDNA-ITS2 gastropod mollusks phylogenetic relationships Aguilera-Muñoz,Felipe Lafarga-Cruz,Fabiola Gallardo-Escárate,Cristian ANALISIS MOLECULAR EN GASTROPODOS CHILENOS COMERCIALES BASADOS EN LAS SECUENCIAS 16S rRNA,. COI Y ITS1-5.8S rDNA-ITS2
description	Gastropod mollusks are part of the principal marine resources cultivated and commercialized in Chile. There are native Chilean species such as loco (Concholepas concholepas), locate (Thais chocolata), trumulco snail (Chorus giganteus), keyhole limpets (Fissurella spp.), tegula snail (Tegula atra) as well as exotic species such as red abalone (Haliotis rufescens) and Japanese abalone (Haliotis discus hannai). Despite their importance as marine resources, molecular genetic studies establishing phylogenetic relationships and estimating population genetic parameters are scarce. The aim of this study is to establish a molecular approach among the main commercial gastropod species in Chile. The mitochondrial genes 16S rRNA and COI, and the nuclear ribosomal region ITSl-5.8SrDNA-ITS2 were amplified by PCR and sequencing. Alignment analysis was used to determine systematic relationships at the specific level for the species studied. The results revealed that 7 species are grouped in 4 genetically distinct families (Haliotidae, Trochidae, Muricidae and Fissurellidae). In comparison with COI sequencing, 16S rRNA and ITSl-5.8SrDNA-ITS2 sequencing were relatively more conserved with a divergence percentage for 16S rDNA and ITSl-5.8SrDNA-ITS2 of 1.2% and 1.8%, respectively, contrasting with the value of 10% obtained for COI in abalone.
author	Aguilera-Muñoz,Felipe Lafarga-Cruz,Fabiola Gallardo-Escárate,Cristian
author_facet	Aguilera-Muñoz,Felipe Lafarga-Cruz,Fabiola Gallardo-Escárate,Cristian
author_sort	Aguilera-Muñoz,Felipe
title	ANALISIS MOLECULAR EN GASTROPODOS CHILENOS COMERCIALES BASADOS EN LAS SECUENCIAS 16S rRNA,. COI Y ITS1-5.8S rDNA-ITS2
title_short	ANALISIS MOLECULAR EN GASTROPODOS CHILENOS COMERCIALES BASADOS EN LAS SECUENCIAS 16S rRNA,. COI Y ITS1-5.8S rDNA-ITS2
title_full	ANALISIS MOLECULAR EN GASTROPODOS CHILENOS COMERCIALES BASADOS EN LAS SECUENCIAS 16S rRNA,. COI Y ITS1-5.8S rDNA-ITS2
title_fullStr	ANALISIS MOLECULAR EN GASTROPODOS CHILENOS COMERCIALES BASADOS EN LAS SECUENCIAS 16S rRNA,. COI Y ITS1-5.8S rDNA-ITS2
title_full_unstemmed	ANALISIS MOLECULAR EN GASTROPODOS CHILENOS COMERCIALES BASADOS EN LAS SECUENCIAS 16S rRNA,. COI Y ITS1-5.8S rDNA-ITS2
title_sort	analisis molecular en gastropodos chilenos comerciales basados en las secuencias 16s rrna,. coi y its1-5.8s rdna-its2
publisher	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382009000100003
work_keys_str_mv	AT aguileramunozfelipe analisismolecularengastropodoschilenoscomercialesbasadosenlassecuencias16srrnacoiyits158srdnaits2 AT lafargacruzfabiola analisismolecularengastropodoschilenoscomercialesbasadosenlassecuencias16srrnacoiyits158srdnaits2 AT gallardoescaratecristian analisismolecularengastropodoschilenoscomercialesbasadosenlassecuencias16srrnacoiyits158srdnaits2
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ANALISIS MOLECULAR EN GASTROPODOS CHILENOS COMERCIALES BASADOS EN LAS SECUENCIAS 16S rRNA,. COI Y ITS1-5.8S rDNA-ITS2

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