First record of flamentous fungi in the coastal upwelling ecosystem off central Chile

We report for the frst time the presence of flamentous fungi in the water column and sediment in the coastal upwelling ecosystem off central Chile, using molecular tools and epifuorescence microscopy. Positive amplifcations of SSU 18s rDNA with specifc fungal primers were obtained for surface waters...

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Autores principales:	Gutiérrez,Marcelo H, Pantoja,Silvio, Quiñones,Renato A, González,Rodrigo R
Lenguaje:	English
Publicado:	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción 2010
Materias:	Fungi upwelling ecosystem central south Chile
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382010000100010
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spelling	oai:scielo:S0717-653820100001000102010-08-09First record of flamentous fungi in the coastal upwelling ecosystem off central ChileGutiérrez,Marcelo HPantoja,SilvioQuiñones,Renato AGonzález,Rodrigo R Fungi upwelling ecosystem central south Chile We report for the frst time the presence of flamentous fungi in the water column and sediment in the coastal upwelling ecosystem off central Chile, using molecular tools and epifuorescence microscopy. Positive amplifcations of SSU 18s rDNA with specifc fungal primers were obtained for surface waters and sediments of this coastal ecosystem. Molecular richness obtained from denaturing gradient gel electrophoresis showed a higher number of fungal genotypes in nearshore than offshore sites and in summer than winter. Fungal structures identifed by epifuorescence microscopy in the water column were present as individual flaments or as aggregates of hyphae. We show for the frst time vertical water column profles of fungal biomass in the marine ecosystem. Fungal biomass reached up to 5 µg CL-1 in surface waters during summer and their vertical patterns agreed with those of chlorophyll-a and with the general distribution of microplankton biomass in the ocean. The presence of viable fungi in the coastal ocean encourages us to decipher their role in the processing of marine organic matter and to evalúate their inclusión in the actual paradigm of the microbial loop and in the biogeochemistry of the oceans.info:eu-repo/semantics/openAccessFacultad de Ciencias Naturales y Oceanográficas, Universidad de ConcepciónGayana (Concepción) v.74 n.1 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382010000100010en10.4067/S0717-65382010000100010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Fungi upwelling ecosystem central south Chile
spellingShingle	Fungi upwelling ecosystem central south Chile Gutiérrez,Marcelo H Pantoja,Silvio Quiñones,Renato A González,Rodrigo R First record of flamentous fungi in the coastal upwelling ecosystem off central Chile
description	We report for the frst time the presence of flamentous fungi in the water column and sediment in the coastal upwelling ecosystem off central Chile, using molecular tools and epifuorescence microscopy. Positive amplifcations of SSU 18s rDNA with specifc fungal primers were obtained for surface waters and sediments of this coastal ecosystem. Molecular richness obtained from denaturing gradient gel electrophoresis showed a higher number of fungal genotypes in nearshore than offshore sites and in summer than winter. Fungal structures identifed by epifuorescence microscopy in the water column were present as individual flaments or as aggregates of hyphae. We show for the frst time vertical water column profles of fungal biomass in the marine ecosystem. Fungal biomass reached up to 5 µg CL-1 in surface waters during summer and their vertical patterns agreed with those of chlorophyll-a and with the general distribution of microplankton biomass in the ocean. The presence of viable fungi in the coastal ocean encourages us to decipher their role in the processing of marine organic matter and to evalúate their inclusión in the actual paradigm of the microbial loop and in the biogeochemistry of the oceans.
author	Gutiérrez,Marcelo H Pantoja,Silvio Quiñones,Renato A González,Rodrigo R
author_facet	Gutiérrez,Marcelo H Pantoja,Silvio Quiñones,Renato A González,Rodrigo R
author_sort	Gutiérrez,Marcelo H
title	First record of flamentous fungi in the coastal upwelling ecosystem off central Chile
title_short	First record of flamentous fungi in the coastal upwelling ecosystem off central Chile
title_full	First record of flamentous fungi in the coastal upwelling ecosystem off central Chile
title_fullStr	First record of flamentous fungi in the coastal upwelling ecosystem off central Chile
title_full_unstemmed	First record of flamentous fungi in the coastal upwelling ecosystem off central Chile
title_sort	first record of flamentous fungi in the coastal upwelling ecosystem off central chile
publisher	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382010000100010
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First record of flamentous fungi in the coastal upwelling ecosystem off central Chile

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