Effect of PHA and LPS on the Activity of Nonspecific Cytotoxic Cells and Macrophages of Rainbow Trout (Oncorhynchus mykiss)

This work studied the effect of the lectin phytohaemagglutinin (PHA) and bacterial lipopolysaccharide (LPS) on the activity of nonspecific cytotoxic cells (NCCs) and macrophages of peripheral blood in the rainbow trout. NCCs are cells of the innate immunity, homologues to Natural Killer (NK) cells o...

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Autores principales:	Gómez,Leonardo A, Cortés,Raúl, Smith,Carlos T
Lenguaje:	English
Publicado:	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción 2013
Materias:	Interleukins innate immunity FasR-FasL lectins leukocytes
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382013000100007
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spelling	oai:scielo:S0717-653820130001000072013-10-15Effect of PHA and LPS on the Activity of Nonspecific Cytotoxic Cells and Macrophages of Rainbow Trout (Oncorhynchus mykiss)Gómez,Leonardo ACortés,RaúlSmith,Carlos T Interleukins innate immunity FasR-FasL lectins leukocytes This work studied the effect of the lectin phytohaemagglutinin (PHA) and bacterial lipopolysaccharide (LPS) on the activity of nonspecific cytotoxic cells (NCCs) and macrophages of peripheral blood in the rainbow trout. NCCs are cells of the innate immunity, homologues to Natural Killer (NK) cells of mammals, capable of eliminating cells infected with intracellular pathogens and also tumoral cells. Their lytic activity is mediated by the perforin/granzyme system and the Fas receptor-Fas ligand (FasR-FasL) system. Activity of NCCs was evaluated by their capacity to lyse HL-60 tumor cells. On the other hand, macrophages eliminate microorganisms by several mechanisms, including the production of reactive oxygen species (ROS) and nitric oxide (NO). Results showed that PHA significantly increased NCCs cytotoxic activity against HL-60 cells, even under conditions in which the perforins/granzymes lytic system should be suppressed, supporting the existence of the FasR-FasL system in trouts. PHA showed no effect on ROS and NO production by macrophages. Finally, LPS showed no effect on the cytotoxic activity of NCCs or in the production of ROS and NO by macrophages.info:eu-repo/semantics/openAccessFacultad de Ciencias Naturales y Oceanográficas, Universidad de ConcepciónGayana (Concepción) v.77 n.1 20132013-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382013000100007en10.4067/S0717-65382013000100007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Interleukins innate immunity FasR-FasL lectins leukocytes
spellingShingle	Interleukins innate immunity FasR-FasL lectins leukocytes Gómez,Leonardo A Cortés,Raúl Smith,Carlos T Effect of PHA and LPS on the Activity of Nonspecific Cytotoxic Cells and Macrophages of Rainbow Trout (Oncorhynchus mykiss)
description	This work studied the effect of the lectin phytohaemagglutinin (PHA) and bacterial lipopolysaccharide (LPS) on the activity of nonspecific cytotoxic cells (NCCs) and macrophages of peripheral blood in the rainbow trout. NCCs are cells of the innate immunity, homologues to Natural Killer (NK) cells of mammals, capable of eliminating cells infected with intracellular pathogens and also tumoral cells. Their lytic activity is mediated by the perforin/granzyme system and the Fas receptor-Fas ligand (FasR-FasL) system. Activity of NCCs was evaluated by their capacity to lyse HL-60 tumor cells. On the other hand, macrophages eliminate microorganisms by several mechanisms, including the production of reactive oxygen species (ROS) and nitric oxide (NO). Results showed that PHA significantly increased NCCs cytotoxic activity against HL-60 cells, even under conditions in which the perforins/granzymes lytic system should be suppressed, supporting the existence of the FasR-FasL system in trouts. PHA showed no effect on ROS and NO production by macrophages. Finally, LPS showed no effect on the cytotoxic activity of NCCs or in the production of ROS and NO by macrophages.
author	Gómez,Leonardo A Cortés,Raúl Smith,Carlos T
author_facet	Gómez,Leonardo A Cortés,Raúl Smith,Carlos T
author_sort	Gómez,Leonardo A
title	Effect of PHA and LPS on the Activity of Nonspecific Cytotoxic Cells and Macrophages of Rainbow Trout (Oncorhynchus mykiss)
title_short	Effect of PHA and LPS on the Activity of Nonspecific Cytotoxic Cells and Macrophages of Rainbow Trout (Oncorhynchus mykiss)
title_full	Effect of PHA and LPS on the Activity of Nonspecific Cytotoxic Cells and Macrophages of Rainbow Trout (Oncorhynchus mykiss)
title_fullStr	Effect of PHA and LPS on the Activity of Nonspecific Cytotoxic Cells and Macrophages of Rainbow Trout (Oncorhynchus mykiss)
title_full_unstemmed	Effect of PHA and LPS on the Activity of Nonspecific Cytotoxic Cells and Macrophages of Rainbow Trout (Oncorhynchus mykiss)
title_sort	effect of pha and lps on the activity of nonspecific cytotoxic cells and macrophages of rainbow trout (oncorhynchus mykiss)
publisher	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382013000100007
work_keys_str_mv	AT gomezleonardoa effectofphaandlpsontheactivityofnonspecificcytotoxiccellsandmacrophagesofrainbowtroutoncorhynchusmykiss AT cortesraul effectofphaandlpsontheactivityofnonspecificcytotoxiccellsandmacrophagesofrainbowtroutoncorhynchusmykiss AT smithcarlost effectofphaandlpsontheactivityofnonspecificcytotoxiccellsandmacrophagesofrainbowtroutoncorhynchusmykiss
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Effect of PHA and LPS on the Activity of Nonspecific Cytotoxic Cells and Macrophages of Rainbow Trout (Oncorhynchus mykiss)

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