Mucus phosphoproteins as an indirect measure of endocrine disruption in native small-bodied freshwater fish, exposed to wastewater treatment plant and pulp and paper mill effluents
ABSTRACT Environmental monitoring programs commonly use fish to study the health of aquatic ecosystems. Nevertheless, lethal sampling techniques are often employed, resulting in ethical considerations. This issue is magnified in Chilean rivers, which contain various endemic fish with conservational...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
2019
|
| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382019000100010 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:scielo:S0717-65382019000100010 |
|---|---|
| record_format |
dspace |
| spelling |
oai:scielo:S0717-653820190001000102019-09-16Mucus phosphoproteins as an indirect measure of endocrine disruption in native small-bodied freshwater fish, exposed to wastewater treatment plant and pulp and paper mill effluentsBahamonde,PaulinaBerrocal,CamilaBarra,RicardoMcmaster,Mark E.Munkittrick,Kelly R.Chiang,Gustavo endocrine disruption fish mucus phosphoproteins vitellogenin ABSTRACT Environmental monitoring programs commonly use fish to study the health of aquatic ecosystems. Nevertheless, lethal sampling techniques are often employed, resulting in ethical considerations. This issue is magnified in Chilean rivers, which contain various endemic fish with conservational concern, according to the International Union for Conservation of Nature Red List of Threatened Species™. Therefore, the aim of this study was to determine if mucosal vitellogenin levels in a native Chilean fish could be used to accurately assess the endocrine disruption potentials of wastewater treatment plant and pulp and paper mill effluents (WWTPEs and PPMEs, respectively). For this, Carmelita de Concepción (Percilia irwini) specimens were exposed WWTPEs and PPEs for 12 days, and mucosal vitellogenin-like phosphoprotein concentrations were determined with a colorimetric assay. Increased VTG-like phosphoproteins and hepatic ethoxyresorufin-o-deethylase induction levels (widely used as biomarker for exposure) were detected in effluent-exposed individuals. This study supports the endocrine disruption potentials of WWTPEs and PPEs in P. irwini. Notably, this is the first study to use non- lethal biomarkers to determine the effects of industrial effluents in a native Chilean freshwater species, thus presenting an alternative vitellogenin-like protein detection method. Nevertheless, additional population and toxicity studies of fish native to Chilean rivers are needed. Further investigation is also required on xeno-oestrogen compounds and on methods for mitigating potential effects on biodiversity.info:eu-repo/semantics/openAccessFacultad de Ciencias Naturales y Oceanográficas, Universidad de ConcepciónGayana (Concepción) v.83 n.1 20192019-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382019000100010en10.4067/S0717-65382019000100010 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
endocrine disruption fish mucus phosphoproteins vitellogenin |
| spellingShingle |
endocrine disruption fish mucus phosphoproteins vitellogenin Bahamonde,Paulina Berrocal,Camila Barra,Ricardo Mcmaster,Mark E. Munkittrick,Kelly R. Chiang,Gustavo Mucus phosphoproteins as an indirect measure of endocrine disruption in native small-bodied freshwater fish, exposed to wastewater treatment plant and pulp and paper mill effluents |
| description |
ABSTRACT Environmental monitoring programs commonly use fish to study the health of aquatic ecosystems. Nevertheless, lethal sampling techniques are often employed, resulting in ethical considerations. This issue is magnified in Chilean rivers, which contain various endemic fish with conservational concern, according to the International Union for Conservation of Nature Red List of Threatened Species™. Therefore, the aim of this study was to determine if mucosal vitellogenin levels in a native Chilean fish could be used to accurately assess the endocrine disruption potentials of wastewater treatment plant and pulp and paper mill effluents (WWTPEs and PPMEs, respectively). For this, Carmelita de Concepción (Percilia irwini) specimens were exposed WWTPEs and PPEs for 12 days, and mucosal vitellogenin-like phosphoprotein concentrations were determined with a colorimetric assay. Increased VTG-like phosphoproteins and hepatic ethoxyresorufin-o-deethylase induction levels (widely used as biomarker for exposure) were detected in effluent-exposed individuals. This study supports the endocrine disruption potentials of WWTPEs and PPEs in P. irwini. Notably, this is the first study to use non- lethal biomarkers to determine the effects of industrial effluents in a native Chilean freshwater species, thus presenting an alternative vitellogenin-like protein detection method. Nevertheless, additional population and toxicity studies of fish native to Chilean rivers are needed. Further investigation is also required on xeno-oestrogen compounds and on methods for mitigating potential effects on biodiversity. |
| author |
Bahamonde,Paulina Berrocal,Camila Barra,Ricardo Mcmaster,Mark E. Munkittrick,Kelly R. Chiang,Gustavo |
| author_facet |
Bahamonde,Paulina Berrocal,Camila Barra,Ricardo Mcmaster,Mark E. Munkittrick,Kelly R. Chiang,Gustavo |
| author_sort |
Bahamonde,Paulina |
| title |
Mucus phosphoproteins as an indirect measure of endocrine disruption in native small-bodied freshwater fish, exposed to wastewater treatment plant and pulp and paper mill effluents |
| title_short |
Mucus phosphoproteins as an indirect measure of endocrine disruption in native small-bodied freshwater fish, exposed to wastewater treatment plant and pulp and paper mill effluents |
| title_full |
Mucus phosphoproteins as an indirect measure of endocrine disruption in native small-bodied freshwater fish, exposed to wastewater treatment plant and pulp and paper mill effluents |
| title_fullStr |
Mucus phosphoproteins as an indirect measure of endocrine disruption in native small-bodied freshwater fish, exposed to wastewater treatment plant and pulp and paper mill effluents |
| title_full_unstemmed |
Mucus phosphoproteins as an indirect measure of endocrine disruption in native small-bodied freshwater fish, exposed to wastewater treatment plant and pulp and paper mill effluents |
| title_sort |
mucus phosphoproteins as an indirect measure of endocrine disruption in native small-bodied freshwater fish, exposed to wastewater treatment plant and pulp and paper mill effluents |
| publisher |
Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción |
| publishDate |
2019 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382019000100010 |
| work_keys_str_mv |
AT bahamondepaulina mucusphosphoproteinsasanindirectmeasureofendocrinedisruptioninnativesmallbodiedfreshwaterfishexposedtowastewatertreatmentplantandpulpandpapermilleffluents AT berrocalcamila mucusphosphoproteinsasanindirectmeasureofendocrinedisruptioninnativesmallbodiedfreshwaterfishexposedtowastewatertreatmentplantandpulpandpapermilleffluents AT barraricardo mucusphosphoproteinsasanindirectmeasureofendocrinedisruptioninnativesmallbodiedfreshwaterfishexposedtowastewatertreatmentplantandpulpandpapermilleffluents AT mcmastermarke mucusphosphoproteinsasanindirectmeasureofendocrinedisruptioninnativesmallbodiedfreshwaterfishexposedtowastewatertreatmentplantandpulpandpapermilleffluents AT munkittrickkellyr mucusphosphoproteinsasanindirectmeasureofendocrinedisruptioninnativesmallbodiedfreshwaterfishexposedtowastewatertreatmentplantandpulpandpapermilleffluents AT chianggustavo mucusphosphoproteinsasanindirectmeasureofendocrinedisruptioninnativesmallbodiedfreshwaterfishexposedtowastewatertreatmentplantandpulpandpapermilleffluents |
| _version_ |
1718442187383898112 |