NEW PERSPECTIVES ON THE DETERMINATION OF PHOSPHATASE ACTIVITY IN ECTOMYCORRHIZAE OF NOTHOFAGUS OBLIQUA FORESTS IN SOUTHERN CHILE

We present a method to localise and quantify surface-bound phosphatase activity (SBPA) in mycorrhizal fungi and mycorrhizae of Nothofagus obliqua (Mirb.) Oerst., using image processed confocal fluorescent microscopy. ELF-97 is a hydrophilic substrate which turns into a strongly fluorescent precipita...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alvarez,Maricel, Härtel,Steffen, Godoy,Roberto, Heyser,Wolfgang
Lenguaje:	English
Publicado:	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción 2003
Materias:	Confocal microscopy ectomycorrhizae of Nothofagus obliqua ELF-97 phosphatase activity quantitative fluorescence microscopy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432003000100007
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-66432003000100007
record_format	dspace
spelling	oai:scielo:S0717-664320030001000072004-02-04NEW PERSPECTIVES ON THE DETERMINATION OF PHOSPHATASE ACTIVITY IN ECTOMYCORRHIZAE OF NOTHOFAGUS OBLIQUA FORESTS IN SOUTHERN CHILEAlvarez,MaricelHärtel,SteffenGodoy,RobertoHeyser,Wolfgang Confocal microscopy ectomycorrhizae of Nothofagus obliqua ELF-97 phosphatase activity quantitative fluorescence microscopy We present a method to localise and quantify surface-bound phosphatase activity (SBPA) in mycorrhizal fungi and mycorrhizae of Nothofagus obliqua (Mirb.) Oerst., using image processed confocal fluorescent microscopy. ELF-97 is a hydrophilic substrate which turns into a strongly fluorescent precipitate upon activation by phosphomonoesterases. In fungal mycelium, this technique has recently been approved by comparison with a standard method (p-nitrophenyl-phosphate). The microscopic technique based on ELF-97 revealed that Paxillus involutus (Batsch: Fr.) Sing. and Austropaxillus boletinoides (Sing.) Brsky. & Jarosch provide different adaptive strategies to changing phosphate concentrations and different pH (3-7). We also analysed SBPA in four mycorrhizal associations of N. obliqua and found that the organisation of the mantle played an essential role regarding the SBPA. In general, mycorrhizal roots shifted SBPA from the root to the mantle. In this context, Pisolithus tinctorius (Pers.) Coker & Couch proved to be the most relevant mycorrhiza partner for N. obliqua by increasing significantly the overall SBPA of the mycorrhiza in respect to non-mycorrhizal roots. In conclusion, ELF-97 fluorescence microscopy in combination with image processing routines determined SBPA and revealed phosphorus (P) adaptation strategies of mycorrhizal fungi and mycorrhizae on a structural-physiological levelinfo:eu-repo/semantics/openAccessFacultad de Ciencias Naturales y Oceanográficas, Universidad de ConcepciónGayana. Botánica v.60 n.1 20032003-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432003000100007en10.4067/S0717-66432003000100007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Confocal microscopy ectomycorrhizae of Nothofagus obliqua ELF-97 phosphatase activity quantitative fluorescence microscopy
spellingShingle	Confocal microscopy ectomycorrhizae of Nothofagus obliqua ELF-97 phosphatase activity quantitative fluorescence microscopy Alvarez,Maricel Härtel,Steffen Godoy,Roberto Heyser,Wolfgang NEW PERSPECTIVES ON THE DETERMINATION OF PHOSPHATASE ACTIVITY IN ECTOMYCORRHIZAE OF NOTHOFAGUS OBLIQUA FORESTS IN SOUTHERN CHILE
description	We present a method to localise and quantify surface-bound phosphatase activity (SBPA) in mycorrhizal fungi and mycorrhizae of Nothofagus obliqua (Mirb.) Oerst., using image processed confocal fluorescent microscopy. ELF-97 is a hydrophilic substrate which turns into a strongly fluorescent precipitate upon activation by phosphomonoesterases. In fungal mycelium, this technique has recently been approved by comparison with a standard method (p-nitrophenyl-phosphate). The microscopic technique based on ELF-97 revealed that Paxillus involutus (Batsch: Fr.) Sing. and Austropaxillus boletinoides (Sing.) Brsky. & Jarosch provide different adaptive strategies to changing phosphate concentrations and different pH (3-7). We also analysed SBPA in four mycorrhizal associations of N. obliqua and found that the organisation of the mantle played an essential role regarding the SBPA. In general, mycorrhizal roots shifted SBPA from the root to the mantle. In this context, Pisolithus tinctorius (Pers.) Coker & Couch proved to be the most relevant mycorrhiza partner for N. obliqua by increasing significantly the overall SBPA of the mycorrhiza in respect to non-mycorrhizal roots. In conclusion, ELF-97 fluorescence microscopy in combination with image processing routines determined SBPA and revealed phosphorus (P) adaptation strategies of mycorrhizal fungi and mycorrhizae on a structural-physiological level
author	Alvarez,Maricel Härtel,Steffen Godoy,Roberto Heyser,Wolfgang
author_facet	Alvarez,Maricel Härtel,Steffen Godoy,Roberto Heyser,Wolfgang
author_sort	Alvarez,Maricel
title	NEW PERSPECTIVES ON THE DETERMINATION OF PHOSPHATASE ACTIVITY IN ECTOMYCORRHIZAE OF NOTHOFAGUS OBLIQUA FORESTS IN SOUTHERN CHILE
title_short	NEW PERSPECTIVES ON THE DETERMINATION OF PHOSPHATASE ACTIVITY IN ECTOMYCORRHIZAE OF NOTHOFAGUS OBLIQUA FORESTS IN SOUTHERN CHILE
title_full	NEW PERSPECTIVES ON THE DETERMINATION OF PHOSPHATASE ACTIVITY IN ECTOMYCORRHIZAE OF NOTHOFAGUS OBLIQUA FORESTS IN SOUTHERN CHILE
title_fullStr	NEW PERSPECTIVES ON THE DETERMINATION OF PHOSPHATASE ACTIVITY IN ECTOMYCORRHIZAE OF NOTHOFAGUS OBLIQUA FORESTS IN SOUTHERN CHILE
title_full_unstemmed	NEW PERSPECTIVES ON THE DETERMINATION OF PHOSPHATASE ACTIVITY IN ECTOMYCORRHIZAE OF NOTHOFAGUS OBLIQUA FORESTS IN SOUTHERN CHILE
title_sort	new perspectives on the determination of phosphatase activity in ectomycorrhizae of nothofagus obliqua forests in southern chile
publisher	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432003000100007
work_keys_str_mv	AT alvarezmaricel newperspectivesonthedeterminationofphosphataseactivityinectomycorrhizaeofnothofagusobliquaforestsinsouthernchile AT hartelsteffen newperspectivesonthedeterminationofphosphataseactivityinectomycorrhizaeofnothofagusobliquaforestsinsouthernchile AT godoyroberto newperspectivesonthedeterminationofphosphataseactivityinectomycorrhizaeofnothofagusobliquaforestsinsouthernchile AT heyserwolfgang newperspectivesonthedeterminationofphosphataseactivityinectomycorrhizaeofnothofagusobliquaforestsinsouthernchile
_version_	1718442210589933568

NEW PERSPECTIVES ON THE DETERMINATION OF PHOSPHATASE ACTIVITY IN ECTOMYCORRHIZAE OF NOTHOFAGUS OBLIQUA FORESTS IN SOUTHERN CHILE

Ejemplares similares