NEW PERSPECTIVES ON THE DETERMINATION OF PHOSPHATASE ACTIVITY IN ECTOMYCORRHIZAE OF NOTHOFAGUS OBLIQUA FORESTS IN SOUTHERN CHILE

NEW PERSPECTIVES ON THE DETERMINATION OF PHOSPHATASE ACTIVITY IN ECTOMYCORRHIZAE OF NOTHOFAGUS OBLIQUA FORESTS IN SOUTHERN CHILE

We present a method to localise and quantify surface-bound phosphatase activity (SBPA) in mycorrhizal fungi and mycorrhizae of Nothofagus obliqua (Mirb.) Oerst., using image processed confocal fluorescent microscopy. ELF-97 is a hydrophilic substrate which turns into a strongly fluorescent precipita...

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Autores principales: Alvarez,Maricel, Härtel,Steffen, Godoy,Roberto, Heyser,Wolfgang
Lenguaje:English
Publicado: Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción 2003
Materias:
Confocal microscopy
ectomycorrhizae of Nothofagus obliqua
ELF-97
phosphatase activity
quantitative fluorescence microscopy
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432003000100007
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