Plant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier & W.E.Higgins

Plant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier & W.E.Higgins

An in vitro culture procedure was developed to induce shoots and roots of Guarianthe skinneri (Orchidaceae) plantlets regenerated from seed-derived protocorms on a Murashige and Skoog (MS) médium supplemented with 6-benzyladenine (BA), indole-3-acetic acid (IAA), a-naftalenacetic acid (NAA) and gibb...

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Autores principales: Coello,Christian Yanelly, Miceli,Clara Luz, Orantes,Carolina, DenDooven,Luc, Gutiérrez,Federico Antonio
Lenguaje:English
Publicado: Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción 2010
Materias:
6-benzyladenine
indole-3-acetic acid
a-naftalenacetic acid
gibberellic acid
shoot induction
rooting
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432010000100003
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spelling oai:scielo:S0717-664320100001000032010-08-12Plant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier & W.E.HigginsCoello,Christian YanellyMiceli,Clara LuzOrantes,CarolinaDenDooven,LucGutiérrez,Federico Antonio 6-benzyladenine indole-3-acetic acid a-naftalenacetic acid gibberellic acid shoot induction rooting An in vitro culture procedure was developed to induce shoots and roots of Guarianthe skinneri (Orchidaceae) plantlets regenerated from seed-derived protocorms on a Murashige and Skoog (MS) médium supplemented with 6-benzyladenine (BA), indole-3-acetic acid (IAA), a-naftalenacetic acid (NAA) and gibberellic acid (GA3). A máximum of 10.6 shoots was obtained with 16.1 μM NAA, 17.1 μM IAA, 6.3 x 10-9 μM GA3 and 0.0023 μM BA. A máximum of 4.0 roots on each shoot with 5.4 μM NAA, 17.1 μM IAA, 0.001 μM AG3 and 4.6 x 10-9 μM BA. Máximum shoot and root length was obtained with a minimum of GA3, but a máximum IAA. GA3, was the main factor controlling shoot and root induction and elongation.info:eu-repo/semantics/openAccessFacultad de Ciencias Naturales y Oceanográficas, Universidad de ConcepciónGayana. Botánica v.67 n.1 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432010000100003en10.4067/S0717-66432010000100003
institution Scielo Chile
collection Scielo Chile
language English
topic 6-benzyladenine
indole-3-acetic acid
a-naftalenacetic acid
gibberellic acid
shoot induction
rooting
spellingShingle 6-benzyladenine
indole-3-acetic acid
a-naftalenacetic acid
gibberellic acid
shoot induction
rooting
Coello,Christian Yanelly
Miceli,Clara Luz
Orantes,Carolina
DenDooven,Luc
Gutiérrez,Federico Antonio
Plant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier & W.E.Higgins
description An in vitro culture procedure was developed to induce shoots and roots of Guarianthe skinneri (Orchidaceae) plantlets regenerated from seed-derived protocorms on a Murashige and Skoog (MS) médium supplemented with 6-benzyladenine (BA), indole-3-acetic acid (IAA), a-naftalenacetic acid (NAA) and gibberellic acid (GA3). A máximum of 10.6 shoots was obtained with 16.1 μM NAA, 17.1 μM IAA, 6.3 x 10-9 μM GA3 and 0.0023 μM BA. A máximum of 4.0 roots on each shoot with 5.4 μM NAA, 17.1 μM IAA, 0.001 μM AG3 and 4.6 x 10-9 μM BA. Máximum shoot and root length was obtained with a minimum of GA3, but a máximum IAA. GA3, was the main factor controlling shoot and root induction and elongation.
author Coello,Christian Yanelly
Miceli,Clara Luz
Orantes,Carolina
DenDooven,Luc
Gutiérrez,Federico Antonio
author_facet Coello,Christian Yanelly
Miceli,Clara Luz
Orantes,Carolina
DenDooven,Luc
Gutiérrez,Federico Antonio
author_sort Coello,Christian Yanelly
title Plant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier & W.E.Higgins
title_short Plant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier & W.E.Higgins
title_full Plant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier & W.E.Higgins
title_fullStr Plant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier & W.E.Higgins
title_full_unstemmed Plant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier & W.E.Higgins
title_sort plant growth regulators optimization for in vitro cultivation of the orchid guarianthe skinneri (bateman) dressier & w.e.higgins
publisher Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
publishDate 2010
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432010000100003
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