Leaf seasonal accumulation of a 47-kDa dehydrin and changes in its cryoprotective activity in Nothofagus dombeyi (Mirb.) Blume

Nothofagus dombeyi (Mirb.) Blume is an evergreen tree, pioneer in habitats subject to unfavorable environmental conditions as nighttime freezing temperatures and low water content. It inhabits zones characterized by cold Winter with freezing temperature and Spring frosts reaching to -5°C. Furthermor...

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Autores principales:	Gallardo-Cerda,Jorge, Bravo,León A, Corcuera,Luis J
Lenguaje:	English
Publicado:	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción 2012
Materias:	Evergreen tree old acclimation proteins
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432012000100002
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spelling	oai:scielo:S0717-664320120001000022014-03-10Leaf seasonal accumulation of a 47-kDa dehydrin and changes in its cryoprotective activity in Nothofagus dombeyi (Mirb.) BlumeGallardo-Cerda,JorgeBravo,León ACorcuera,Luis J Evergreen tree old acclimation proteins Nothofagus dombeyi (Mirb.) Blume is an evergreen tree, pioneer in habitats subject to unfavorable environmental conditions as nighttime freezing temperatures and low water content. It inhabits zones characterized by cold Winter with freezing temperature and Spring frosts reaching to -5°C. Furthermore, it has the capacity to maintain a functional photosynthetic apparatus at low temperatures. We postulated that N. dombeyi is capable to accumulate dehydrins in leaves in response to cold. Seasonal variation in dehydrins, soluble total proteins, and cryoprotective activity in vitro of protein extracts of N. dombeyi leaves were analyzed during the years 2004 and 2005. A dehydrin-like protein of 47-kDa was immunologically identified; this protein was present only in Winter and Spring. The highest accumulation of this protein and the maximal in vitro cryoprotective activity was found during Spring. The leaf total protein extract of this season preserved the 100% of lactate dehydrogenase (LDH) enzyme activity after 5 freeze/thaw cycles in liquid nitrogen adding 10 μg/ml of total proteins extract. The soluble protein concentration increased from 1.8 mg/g in Summer to 4.8 mg/g in Fall on base of fresh weight, and declined through Winter and Spring. The 47-kDa dehydrin may be related to cold hardiness of leaf tissue in overwintering N. dombeyi. We conclude that cell dehydration caused by freezing is the main factor involved in the triggering of 47-kDa dehydrin accumulation in leaves of N. dombeyi. The accumulation of reactive oxygen species (ROS) by oxidative stress product of high irradiance and frosts during Spring, and their role in the triggering of the 47-kDa dehydrin accumulation in leaves of N. dombeyi is discussed.info:eu-repo/semantics/openAccessFacultad de Ciencias Naturales y Oceanográficas, Universidad de ConcepciónGayana. Botánica v.69 n.1 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432012000100002en10.4067/S0717-66432012000100002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Evergreen tree old acclimation proteins
spellingShingle	Evergreen tree old acclimation proteins Gallardo-Cerda,Jorge Bravo,León A Corcuera,Luis J Leaf seasonal accumulation of a 47-kDa dehydrin and changes in its cryoprotective activity in Nothofagus dombeyi (Mirb.) Blume
description	Nothofagus dombeyi (Mirb.) Blume is an evergreen tree, pioneer in habitats subject to unfavorable environmental conditions as nighttime freezing temperatures and low water content. It inhabits zones characterized by cold Winter with freezing temperature and Spring frosts reaching to -5°C. Furthermore, it has the capacity to maintain a functional photosynthetic apparatus at low temperatures. We postulated that N. dombeyi is capable to accumulate dehydrins in leaves in response to cold. Seasonal variation in dehydrins, soluble total proteins, and cryoprotective activity in vitro of protein extracts of N. dombeyi leaves were analyzed during the years 2004 and 2005. A dehydrin-like protein of 47-kDa was immunologically identified; this protein was present only in Winter and Spring. The highest accumulation of this protein and the maximal in vitro cryoprotective activity was found during Spring. The leaf total protein extract of this season preserved the 100% of lactate dehydrogenase (LDH) enzyme activity after 5 freeze/thaw cycles in liquid nitrogen adding 10 μg/ml of total proteins extract. The soluble protein concentration increased from 1.8 mg/g in Summer to 4.8 mg/g in Fall on base of fresh weight, and declined through Winter and Spring. The 47-kDa dehydrin may be related to cold hardiness of leaf tissue in overwintering N. dombeyi. We conclude that cell dehydration caused by freezing is the main factor involved in the triggering of 47-kDa dehydrin accumulation in leaves of N. dombeyi. The accumulation of reactive oxygen species (ROS) by oxidative stress product of high irradiance and frosts during Spring, and their role in the triggering of the 47-kDa dehydrin accumulation in leaves of N. dombeyi is discussed.
author	Gallardo-Cerda,Jorge Bravo,León A Corcuera,Luis J
author_facet	Gallardo-Cerda,Jorge Bravo,León A Corcuera,Luis J
author_sort	Gallardo-Cerda,Jorge
title	Leaf seasonal accumulation of a 47-kDa dehydrin and changes in its cryoprotective activity in Nothofagus dombeyi (Mirb.) Blume
title_short	Leaf seasonal accumulation of a 47-kDa dehydrin and changes in its cryoprotective activity in Nothofagus dombeyi (Mirb.) Blume
title_full	Leaf seasonal accumulation of a 47-kDa dehydrin and changes in its cryoprotective activity in Nothofagus dombeyi (Mirb.) Blume
title_fullStr	Leaf seasonal accumulation of a 47-kDa dehydrin and changes in its cryoprotective activity in Nothofagus dombeyi (Mirb.) Blume
title_full_unstemmed	Leaf seasonal accumulation of a 47-kDa dehydrin and changes in its cryoprotective activity in Nothofagus dombeyi (Mirb.) Blume
title_sort	leaf seasonal accumulation of a 47-kda dehydrin and changes in its cryoprotective activity in nothofagus dombeyi (mirb.) blume
publisher	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432012000100002
work_keys_str_mv	AT gallardocerdajorge leafseasonalaccumulationofa47kdadehydrinandchangesinitscryoprotectiveactivityinnothofagusdombeyimirbblume AT bravoleona leafseasonalaccumulationofa47kdadehydrinandchangesinitscryoprotectiveactivityinnothofagusdombeyimirbblume AT corcueraluisj leafseasonalaccumulationofa47kdadehydrinandchangesinitscryoprotectiveactivityinnothofagusdombeyimirbblume
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Leaf seasonal accumulation of a 47-kDa dehydrin and changes in its cryoprotective activity in Nothofagus dombeyi (Mirb.) Blume

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