Plant growth regulators optimization for maximize shoots number in Agave americana L. by indirect organogenesis

Current protocols for Agave americana L. micropropagation have limited commercial application due to the low number of plants produced by explant. Indirect organogenesis could be an alternative, however is necessary to optimize plant growth regulators for plantlet number maximization. The objective...

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Autores principales:	REYES-ZAMBRANO,SHEILA JAZMÍN, LECONA-GUZMÁN,CARLOS ALBERTO, BARREDO-POOL,FELIPE ALONSO, AMBROSIO CALDERÓN,JOSÉ DOLORES, ABUD-ARCHILA,MIGUEL, RINCÓN-ROSALES,REINER, RUIZ-VALDIVIEZO,VÍCTOR MANUEL, GUTIÉRREZ-MICELI,FEDERICO ANTONIO
Lenguaje:	English
Publicado:	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción 2016
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432016000100014
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spelling	oai:scielo:S0717-664320160001000142016-08-30Plant growth regulators optimization for maximize shoots number in Agave americana L. by indirect organogenesisREYES-ZAMBRANO,SHEILA JAZMÍNLECONA-GUZMÁN,CARLOS ALBERTOBARREDO-POOL,FELIPE ALONSOAMBROSIO CALDERÓN,JOSÉ DOLORESABUD-ARCHILA,MIGUELRINCÓN-ROSALES,REINERRUIZ-VALDIVIEZO,VÍCTOR MANUELGUTIÉRREZ-MICELI,FEDERICO ANTONIO Current protocols for Agave americana L. micropropagation have limited commercial application due to the low number of plants produced by explant. Indirect organogenesis could be an alternative, however is necessary to optimize plant growth regulators for plantlet number maximization. The objective of this work was to optimize 2,4-dicholorophenoxyacetic acid (2,4-D) and 6-benzyl adenine (BA) concentrations on the induction of A. americana embryogenic callus from apical meristem as explant for maximized the number of shoots per callus using a response surface experimental design. MS medium containing 30 g l-1 sucrose amended with 0.11,0.18, 0.45 or 2.26 μM 2,4-D, and 11.0, 22.0, 38.2 or 44.0 μM BA was used. Nine treatments with three repetitions was applied and number of shoots per callus were monitored after 4, 16, 20 and 36 weeks. In vitro rooting of shoot was done in MS medium added with indole butyric acid (IBA). A maximum number of plantlets per explant (74) was obtained with 2.26 μM of 2,4-D and 38.2 μM BA. In conclusion, the indirect organogenesis of A. americana L. could be an alternative for obtain plantlets for propagation commercial purposes.info:eu-repo/semantics/openAccessFacultad de Ciencias Naturales y Oceanográficas, Universidad de ConcepciónGayana. Botánica v.73 n.1 20162016-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432016000100014en10.4067/S0717-66432016000100014
institution	Scielo Chile
collection	Scielo Chile
language	English
description	Current protocols for Agave americana L. micropropagation have limited commercial application due to the low number of plants produced by explant. Indirect organogenesis could be an alternative, however is necessary to optimize plant growth regulators for plantlet number maximization. The objective of this work was to optimize 2,4-dicholorophenoxyacetic acid (2,4-D) and 6-benzyl adenine (BA) concentrations on the induction of A. americana embryogenic callus from apical meristem as explant for maximized the number of shoots per callus using a response surface experimental design. MS medium containing 30 g l-1 sucrose amended with 0.11,0.18, 0.45 or 2.26 μM 2,4-D, and 11.0, 22.0, 38.2 or 44.0 μM BA was used. Nine treatments with three repetitions was applied and number of shoots per callus were monitored after 4, 16, 20 and 36 weeks. In vitro rooting of shoot was done in MS medium added with indole butyric acid (IBA). A maximum number of plantlets per explant (74) was obtained with 2.26 μM of 2,4-D and 38.2 μM BA. In conclusion, the indirect organogenesis of A. americana L. could be an alternative for obtain plantlets for propagation commercial purposes.
author	REYES-ZAMBRANO,SHEILA JAZMÍN LECONA-GUZMÁN,CARLOS ALBERTO BARREDO-POOL,FELIPE ALONSO AMBROSIO CALDERÓN,JOSÉ DOLORES ABUD-ARCHILA,MIGUEL RINCÓN-ROSALES,REINER RUIZ-VALDIVIEZO,VÍCTOR MANUEL GUTIÉRREZ-MICELI,FEDERICO ANTONIO
spellingShingle	REYES-ZAMBRANO,SHEILA JAZMÍN LECONA-GUZMÁN,CARLOS ALBERTO BARREDO-POOL,FELIPE ALONSO AMBROSIO CALDERÓN,JOSÉ DOLORES ABUD-ARCHILA,MIGUEL RINCÓN-ROSALES,REINER RUIZ-VALDIVIEZO,VÍCTOR MANUEL GUTIÉRREZ-MICELI,FEDERICO ANTONIO Plant growth regulators optimization for maximize shoots number in Agave americana L. by indirect organogenesis
author_facet	REYES-ZAMBRANO,SHEILA JAZMÍN LECONA-GUZMÁN,CARLOS ALBERTO BARREDO-POOL,FELIPE ALONSO AMBROSIO CALDERÓN,JOSÉ DOLORES ABUD-ARCHILA,MIGUEL RINCÓN-ROSALES,REINER RUIZ-VALDIVIEZO,VÍCTOR MANUEL GUTIÉRREZ-MICELI,FEDERICO ANTONIO
author_sort	REYES-ZAMBRANO,SHEILA JAZMÍN
title	Plant growth regulators optimization for maximize shoots number in Agave americana L. by indirect organogenesis
title_short	Plant growth regulators optimization for maximize shoots number in Agave americana L. by indirect organogenesis
title_full	Plant growth regulators optimization for maximize shoots number in Agave americana L. by indirect organogenesis
title_fullStr	Plant growth regulators optimization for maximize shoots number in Agave americana L. by indirect organogenesis
title_full_unstemmed	Plant growth regulators optimization for maximize shoots number in Agave americana L. by indirect organogenesis
title_sort	plant growth regulators optimization for maximize shoots number in agave americana l. by indirect organogenesis
publisher	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432016000100014
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Plant growth regulators optimization for maximize shoots number in Agave americana L. by indirect organogenesis

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