Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav.

ABSTRACT The present work explores in vivo physiological, morphological and chemical features during full hydration, desiccation and rehydration of the filmy fern Hymenophyllum dentatum with two main objectives: 1) to get further insight about the mechanisms underlying its desiccation tolerance, and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bravo,Soraya, Parra,María José, Castillo,Rosario, Sepúlveda,Francisco, Turner,Aileen, Bertín,Ariana, Osorio,Germán, Tereszczuk,Joanna, Bruna,Carola, Hasbún,Rodrigo
Lenguaje:	English
Publicado:	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción 2016
Materias:	Hymenophyllum desiccation tolerance confocal 3D microscopy FT-IR microspectroscopy rapid dehydration/rehydration
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432016000200402
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-66432016000200402
record_format	dspace
spelling	oai:scielo:S0717-664320160002004022017-03-09Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav.Bravo,SorayaParra,María JoséCastillo,RosarioSepúlveda,FranciscoTurner,AileenBertín,ArianaOsorio,GermánTereszczuk,JoannaBruna,CarolaHasbún,Rodrigo Hymenophyllum desiccation tolerance confocal 3D microscopy FT-IR microspectroscopy rapid dehydration/rehydration ABSTRACT The present work explores in vivo physiological, morphological and chemical features during full hydration, desiccation and rehydration of the filmy fern Hymenophyllum dentatum with two main objectives: 1) to get further insight about the mechanisms underlying its desiccation tolerance, and 2) to understand how this plant manages mechanical stress induced by water loss and recovery. With these purposes, physiological (relative water content and F /F chlorophyll fluorescence parameter), morphological (Confocal Laser Scanning Microscopy and 3D reconstruction) and chemical (FT- IR microspectroscopy) data were obtained and compared between fully hydrated, desiccated and rehydrated tissues of H. dentatum. Remarkable changes in cell architecture and chemical composition were observed in vivo in desiccated leaves. Cells were smaller, showed a collapsed general appearance, and were delimitated by apparently folded cell walls. Marked changes in chloroplasts location and decrease in the number of active chloroplasts were also evidenced. Chemical experiments showed that changes in the secondary structure of proteins and in the polysaccharide composition of the cell wall occur in desiccated cells. All changes were rapidly reversed upon rehydration. This study shows that H. dentatum presents an extreme case of desiccation tolerance, able to withdraw severe, rapid and consecutive dehydration/rehydration induced stress by the function of constitutive systems of protection and reparation, in which cell wall folding plays a relevant role as a protective system against mechanical and oxidative stress. Besides, H. dentatum is proposed as an excellent plant model for the study of dissection tolerance such as one cell layer fern, auto-fluorescence of cellular compartments, and simple long term storage under laboratory conditions, among others.info:eu-repo/semantics/openAccessFacultad de Ciencias Naturales y Oceanográficas, Universidad de ConcepciónGayana. Botánica v.73 n.2 20162016-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432016000200402en10.4067/S0717-66432016000200402
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Hymenophyllum desiccation tolerance confocal 3D microscopy FT-IR microspectroscopy rapid dehydration/rehydration
spellingShingle	Hymenophyllum desiccation tolerance confocal 3D microscopy FT-IR microspectroscopy rapid dehydration/rehydration Bravo,Soraya Parra,María José Castillo,Rosario Sepúlveda,Francisco Turner,Aileen Bertín,Ariana Osorio,Germán Tereszczuk,Joanna Bruna,Carola Hasbún,Rodrigo Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav.
description	ABSTRACT The present work explores in vivo physiological, morphological and chemical features during full hydration, desiccation and rehydration of the filmy fern Hymenophyllum dentatum with two main objectives: 1) to get further insight about the mechanisms underlying its desiccation tolerance, and 2) to understand how this plant manages mechanical stress induced by water loss and recovery. With these purposes, physiological (relative water content and F /F chlorophyll fluorescence parameter), morphological (Confocal Laser Scanning Microscopy and 3D reconstruction) and chemical (FT- IR microspectroscopy) data were obtained and compared between fully hydrated, desiccated and rehydrated tissues of H. dentatum. Remarkable changes in cell architecture and chemical composition were observed in vivo in desiccated leaves. Cells were smaller, showed a collapsed general appearance, and were delimitated by apparently folded cell walls. Marked changes in chloroplasts location and decrease in the number of active chloroplasts were also evidenced. Chemical experiments showed that changes in the secondary structure of proteins and in the polysaccharide composition of the cell wall occur in desiccated cells. All changes were rapidly reversed upon rehydration. This study shows that H. dentatum presents an extreme case of desiccation tolerance, able to withdraw severe, rapid and consecutive dehydration/rehydration induced stress by the function of constitutive systems of protection and reparation, in which cell wall folding plays a relevant role as a protective system against mechanical and oxidative stress. Besides, H. dentatum is proposed as an excellent plant model for the study of dissection tolerance such as one cell layer fern, auto-fluorescence of cellular compartments, and simple long term storage under laboratory conditions, among others.
author	Bravo,Soraya Parra,María José Castillo,Rosario Sepúlveda,Francisco Turner,Aileen Bertín,Ariana Osorio,Germán Tereszczuk,Joanna Bruna,Carola Hasbún,Rodrigo
author_facet	Bravo,Soraya Parra,María José Castillo,Rosario Sepúlveda,Francisco Turner,Aileen Bertín,Ariana Osorio,Germán Tereszczuk,Joanna Bruna,Carola Hasbún,Rodrigo
author_sort	Bravo,Soraya
title	Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav.
title_short	Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav.
title_full	Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav.
title_fullStr	Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav.
title_full_unstemmed	Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav.
title_sort	reversible in vivo cellular changes occur during desiccation and recovery: desiccation tolerance of the resurrection filmy fern hymenophyllum dentatum cav.
publisher	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432016000200402
work_keys_str_mv	AT bravosoraya reversibleinvivocellularchangesoccurduringdesiccationandrecoverydesiccationtoleranceoftheresurrectionfilmyfernhymenophyllumdentatumcav AT parramariajose reversibleinvivocellularchangesoccurduringdesiccationandrecoverydesiccationtoleranceoftheresurrectionfilmyfernhymenophyllumdentatumcav AT castillorosario reversibleinvivocellularchangesoccurduringdesiccationandrecoverydesiccationtoleranceoftheresurrectionfilmyfernhymenophyllumdentatumcav AT sepulvedafrancisco reversibleinvivocellularchangesoccurduringdesiccationandrecoverydesiccationtoleranceoftheresurrectionfilmyfernhymenophyllumdentatumcav AT turneraileen reversibleinvivocellularchangesoccurduringdesiccationandrecoverydesiccationtoleranceoftheresurrectionfilmyfernhymenophyllumdentatumcav AT bertinariana reversibleinvivocellularchangesoccurduringdesiccationandrecoverydesiccationtoleranceoftheresurrectionfilmyfernhymenophyllumdentatumcav AT osoriogerman reversibleinvivocellularchangesoccurduringdesiccationandrecoverydesiccationtoleranceoftheresurrectionfilmyfernhymenophyllumdentatumcav AT tereszczukjoanna reversibleinvivocellularchangesoccurduringdesiccationandrecoverydesiccationtoleranceoftheresurrectionfilmyfernhymenophyllumdentatumcav AT brunacarola reversibleinvivocellularchangesoccurduringdesiccationandrecoverydesiccationtoleranceoftheresurrectionfilmyfernhymenophyllumdentatumcav AT hasbunrodrigo reversibleinvivocellularchangesoccurduringdesiccationandrecoverydesiccationtoleranceoftheresurrectionfilmyfernhymenophyllumdentatumcav
_version_	1718442323636912128

Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav.

Ejemplares similares