Asymbiotic germination in three Chloraea species (Orchidaceae) from Chile

ABSTRACT Orchids require symbiotic fungi and/or specific conditions to germinate. Asymbiotic techniques have been shown successful for orchid germination. In Chile, Chloraea include many endemic, and potentially ornamental, terrestrial orchid species. In this study, individuals of Chloraea crispa, C...

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Autores principales:	PEREIRA,GUILLERMO, ALBORNOZ,VERÓNICA, ROMERO,CHRISTIAN, LARA,SEBASTIÁN, SÁNCHEZ-OLATE,MANUEL, RÍOS,DARCY, ATALA,CRISTIAN
Lenguaje:	English
Publicado:	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción 2017
Materias:	Terrestrial orchids endemic species in vitro germination culture media orchid propagation asymbiotic germination
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432017000100131
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spelling	oai:scielo:S0717-664320170001001312017-09-04Asymbiotic germination in three Chloraea species (Orchidaceae) from ChilePEREIRA,GUILLERMOALBORNOZ,VERÓNICAROMERO,CHRISTIANLARA,SEBASTIÁNSÁNCHEZ-OLATE,MANUELRÍOS,DARCYATALA,CRISTIAN Terrestrial orchids endemic species in vitro germination culture media orchid propagation asymbiotic germination ABSTRACT Orchids require symbiotic fungi and/or specific conditions to germinate. Asymbiotic techniques have been shown successful for orchid germination. In Chile, Chloraea include many endemic, and potentially ornamental, terrestrial orchid species. In this study, individuals of Chloraea crispa, C. gavilu and C. virescens were manually autopollinated. The resulting capsules were sterilized and seeds were aseptically obtained. We evaluated asymbiotic germination in: Agar Water (AW), Knudson C (KC), Banana Culture Media (CMB), Tomato Culture Media (CMT), Malmgren Modified (MM), Murashige and Skoog (MS), and MS modified (MS1/2). Seeds were incubated in the dark at 24±1 ºC for 2 weeks. Then they were put in 16/8 h light/dark cycles for 14 weeks. We registered germination and embryo development in the different culture media. After 8 weeks, all tested Chloraea species germinated in most culture media. After 16 weeks, embryos in MM showed an evident shoot. In AW only the pre-germination stage was achieved. In C. gavilu germination was over 90% in MM, CMT, and CMB. In KC, a lower germination was obtained in all tested species compared to the other culture media, except for AW. Asymbiotic germination of endemic Chilean orchids could be a viable strategy for their conservation and propagation as ornamental species.info:eu-repo/semantics/openAccessFacultad de Ciencias Naturales y Oceanográficas, Universidad de ConcepciónGayana. Botánica v.74 n.1 20172017-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432017000100131en10.4067/S0717-66432017005000107
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Terrestrial orchids endemic species in vitro germination culture media orchid propagation asymbiotic germination
spellingShingle	Terrestrial orchids endemic species in vitro germination culture media orchid propagation asymbiotic germination PEREIRA,GUILLERMO ALBORNOZ,VERÓNICA ROMERO,CHRISTIAN LARA,SEBASTIÁN SÁNCHEZ-OLATE,MANUEL RÍOS,DARCY ATALA,CRISTIAN Asymbiotic germination in three Chloraea species (Orchidaceae) from Chile
description	ABSTRACT Orchids require symbiotic fungi and/or specific conditions to germinate. Asymbiotic techniques have been shown successful for orchid germination. In Chile, Chloraea include many endemic, and potentially ornamental, terrestrial orchid species. In this study, individuals of Chloraea crispa, C. gavilu and C. virescens were manually autopollinated. The resulting capsules were sterilized and seeds were aseptically obtained. We evaluated asymbiotic germination in: Agar Water (AW), Knudson C (KC), Banana Culture Media (CMB), Tomato Culture Media (CMT), Malmgren Modified (MM), Murashige and Skoog (MS), and MS modified (MS1/2). Seeds were incubated in the dark at 24±1 ºC for 2 weeks. Then they were put in 16/8 h light/dark cycles for 14 weeks. We registered germination and embryo development in the different culture media. After 8 weeks, all tested Chloraea species germinated in most culture media. After 16 weeks, embryos in MM showed an evident shoot. In AW only the pre-germination stage was achieved. In C. gavilu germination was over 90% in MM, CMT, and CMB. In KC, a lower germination was obtained in all tested species compared to the other culture media, except for AW. Asymbiotic germination of endemic Chilean orchids could be a viable strategy for their conservation and propagation as ornamental species.
author	PEREIRA,GUILLERMO ALBORNOZ,VERÓNICA ROMERO,CHRISTIAN LARA,SEBASTIÁN SÁNCHEZ-OLATE,MANUEL RÍOS,DARCY ATALA,CRISTIAN
author_facet	PEREIRA,GUILLERMO ALBORNOZ,VERÓNICA ROMERO,CHRISTIAN LARA,SEBASTIÁN SÁNCHEZ-OLATE,MANUEL RÍOS,DARCY ATALA,CRISTIAN
author_sort	PEREIRA,GUILLERMO
title	Asymbiotic germination in three Chloraea species (Orchidaceae) from Chile
title_short	Asymbiotic germination in three Chloraea species (Orchidaceae) from Chile
title_full	Asymbiotic germination in three Chloraea species (Orchidaceae) from Chile
title_fullStr	Asymbiotic germination in three Chloraea species (Orchidaceae) from Chile
title_full_unstemmed	Asymbiotic germination in three Chloraea species (Orchidaceae) from Chile
title_sort	asymbiotic germination in three chloraea species (orchidaceae) from chile
publisher	Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432017000100131
work_keys_str_mv	AT pereiraguillermo asymbioticgerminationinthreechloraeaspeciesorchidaceaefromchile AT albornozveronica asymbioticgerminationinthreechloraeaspeciesorchidaceaefromchile AT romerochristian asymbioticgerminationinthreechloraeaspeciesorchidaceaefromchile AT larasebastian asymbioticgerminationinthreechloraeaspeciesorchidaceaefromchile AT sanchezolatemanuel asymbioticgerminationinthreechloraeaspeciesorchidaceaefromchile AT riosdarcy asymbioticgerminationinthreechloraeaspeciesorchidaceaefromchile AT atalacristian asymbioticgerminationinthreechloraeaspeciesorchidaceaefromchile
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Asymbiotic germination in three Chloraea species (Orchidaceae) from Chile

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