Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communities

Diatoms play a decisive role in global primary production, have a key role in the marine food web, and serve as food for the industrial culture of aquatic animals. In addition, species of diatoms have been chosen as tools for measuring sea water quality and for bioassay procedures. Among them, Phaeo...

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Autores principales:	Soto,Katia, Collantes,Gloria, Zahr,Marcela, Kuznar,Juan
Lenguaje:	English
Publicado:	Escuela de Ciencias del Mar. Pontificia Universidad Católica de Valparaíso 2005
Materias:	microscopic algae bacteria growth enumeration epifluorescence
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-71782005000200002
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spelling	oai:scielo:S0717-717820050002000022007-12-12Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communitiesSoto,KatiaCollantes,GloriaZahr,MarcelaKuznar,Juan microscopic algae bacteria growth enumeration epifluorescence Diatoms play a decisive role in global primary production, have a key role in the marine food web, and serve as food for the industrial culture of aquatic animals. In addition, species of diatoms have been chosen as tools for measuring sea water quality and for bioassay procedures. Among them, Phaeodactylum tricornutum (Bohlin) Lewin, 1958, represents a good biological model due to its ease of handling and also to its physiological, genetic and growth characteristics. We investigated the use of the Epifluorescence Microscopy (EPM) to visualize and enumerate P. tricornutum cells and bacteria in cultures containing both kinds of microorganisms. The cell suspension was filtered, stained with SYBR Green and analyzed by EPM. P. tricornutum can be enumerated using EPM with comparable accuracy as using a hemocytometer. The same kind of growth curve is obtained with either method. EPM is an advantageous alternative to the classic light microscope counting method; it allows the simultaneous enumeration of P. tricornutum and bacteria in a single sample, measuring red autofluorescence and green fluorescence respectively. Moreover, the methodology is suitable for dynamic studies performed to determine the growth dependence between P. tricornutum and bacteria. Another advantage of this technique is that different shapes, sizes and physiological stages of the microbiological communities can be observedinfo:eu-repo/semantics/openAccessEscuela de Ciencias del Mar. Pontificia Universidad Católica de ValparaísoInvestigaciones marinas v.33 n.2 20052005-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-71782005000200002en10.4067/S0717-71782005000200002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	microscopic algae bacteria growth enumeration epifluorescence
spellingShingle	microscopic algae bacteria growth enumeration epifluorescence Soto,Katia Collantes,Gloria Zahr,Marcela Kuznar,Juan Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communities
description	Diatoms play a decisive role in global primary production, have a key role in the marine food web, and serve as food for the industrial culture of aquatic animals. In addition, species of diatoms have been chosen as tools for measuring sea water quality and for bioassay procedures. Among them, Phaeodactylum tricornutum (Bohlin) Lewin, 1958, represents a good biological model due to its ease of handling and also to its physiological, genetic and growth characteristics. We investigated the use of the Epifluorescence Microscopy (EPM) to visualize and enumerate P. tricornutum cells and bacteria in cultures containing both kinds of microorganisms. The cell suspension was filtered, stained with SYBR Green and analyzed by EPM. P. tricornutum can be enumerated using EPM with comparable accuracy as using a hemocytometer. The same kind of growth curve is obtained with either method. EPM is an advantageous alternative to the classic light microscope counting method; it allows the simultaneous enumeration of P. tricornutum and bacteria in a single sample, measuring red autofluorescence and green fluorescence respectively. Moreover, the methodology is suitable for dynamic studies performed to determine the growth dependence between P. tricornutum and bacteria. Another advantage of this technique is that different shapes, sizes and physiological stages of the microbiological communities can be observed
author	Soto,Katia Collantes,Gloria Zahr,Marcela Kuznar,Juan
author_facet	Soto,Katia Collantes,Gloria Zahr,Marcela Kuznar,Juan
author_sort	Soto,Katia
title	Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communities
title_short	Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communities
title_full	Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communities
title_fullStr	Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communities
title_full_unstemmed	Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communities
title_sort	simultaneous enumeration of phaeodactylum tricornutum (mlb292) and bacteria growing in mixed communities
publisher	Escuela de Ciencias del Mar. Pontificia Universidad Católica de Valparaíso
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-71782005000200002
work_keys_str_mv	AT sotokatia simultaneousenumerationofphaeodactylumtricornutummlb292andbacteriagrowinginmixedcommunities AT collantesgloria simultaneousenumerationofphaeodactylumtricornutummlb292andbacteriagrowinginmixedcommunities AT zahrmarcela simultaneousenumerationofphaeodactylumtricornutummlb292andbacteriagrowinginmixedcommunities AT kuznarjuan simultaneousenumerationofphaeodactylumtricornutummlb292andbacteriagrowinginmixedcommunities
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Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communities

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