OCHRATOXIN-A DETERMINATION BY HPLC WITH FLUORESCENCE DETECTION (HPLC-FL): A NEW STANDARDIZATION METHOD FOR WHEAT SAMPLES

The main objective of this study was to evaluate the presence of Ochratoxin A (OTA) in wheat grains and wheat flour samples using a new high performance liquid chromatography (HPLC) method. The experiment used six wheat grain samples from different industry storage place from Chapeco (SC), South Bra...

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Autores principales:	Teixeira,Mario L, Pertuzzatti,Daiane, Leite,Débora C, Oliveira,Luis Flavio S, Fuentefria,Alexandre M
Lenguaje:	English
Publicado:	Sociedad Chilena de Nutrición, Bromatología y Toxicología 2010
Materias:	Ochratoxin A HPLC fluorometric methods wheat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-75182010000200007
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spelling	oai:scielo:S0717-751820100002000072010-12-06OCHRATOXIN-A DETERMINATION BY HPLC WITH FLUORESCENCE DETECTION (HPLC-FL): A NEW STANDARDIZATION METHOD FOR WHEAT SAMPLESTeixeira,Mario LPertuzzatti,DaianeLeite,Débora COliveira,Luis Flavio SFuentefria,Alexandre M Ochratoxin A HPLC fluorometric methods wheat The main objective of this study was to evaluate the presence of Ochratoxin A (OTA) in wheat grains and wheat flour samples using a new high performance liquid chromatography (HPLC) method. The experiment used six wheat grain samples from different industry storage place from Chapeco (SC), South Brazil, on August 2008. The OTA extraction was carried out using acetonitrile: water (120:80 v/v) as solvent. Thereafter, the supernatant was filtered, and applied on OTA-specific immunoafinity column to HPLC Furthermore, the column was washed with water and the toxin was eluted with methanol. The OTA wheat grains and wheat flour concentration were analyzed by a fluorescence detector coupled to the HPLC apparatus. The results showed a smaller OTA concentration than the limits set by international legislation.info:eu-repo/semantics/openAccessSociedad Chilena de Nutrición, Bromatología y ToxicologíaRevista chilena de nutrición v.37 n.2 20102010-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-75182010000200007en10.4067/S0717-75182010000200007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Ochratoxin A HPLC fluorometric methods wheat
spellingShingle	Ochratoxin A HPLC fluorometric methods wheat Teixeira,Mario L Pertuzzatti,Daiane Leite,Débora C Oliveira,Luis Flavio S Fuentefria,Alexandre M OCHRATOXIN-A DETERMINATION BY HPLC WITH FLUORESCENCE DETECTION (HPLC-FL): A NEW STANDARDIZATION METHOD FOR WHEAT SAMPLES
description	The main objective of this study was to evaluate the presence of Ochratoxin A (OTA) in wheat grains and wheat flour samples using a new high performance liquid chromatography (HPLC) method. The experiment used six wheat grain samples from different industry storage place from Chapeco (SC), South Brazil, on August 2008. The OTA extraction was carried out using acetonitrile: water (120:80 v/v) as solvent. Thereafter, the supernatant was filtered, and applied on OTA-specific immunoafinity column to HPLC Furthermore, the column was washed with water and the toxin was eluted with methanol. The OTA wheat grains and wheat flour concentration were analyzed by a fluorescence detector coupled to the HPLC apparatus. The results showed a smaller OTA concentration than the limits set by international legislation.
author	Teixeira,Mario L Pertuzzatti,Daiane Leite,Débora C Oliveira,Luis Flavio S Fuentefria,Alexandre M
author_facet	Teixeira,Mario L Pertuzzatti,Daiane Leite,Débora C Oliveira,Luis Flavio S Fuentefria,Alexandre M
author_sort	Teixeira,Mario L
title	OCHRATOXIN-A DETERMINATION BY HPLC WITH FLUORESCENCE DETECTION (HPLC-FL): A NEW STANDARDIZATION METHOD FOR WHEAT SAMPLES
title_short	OCHRATOXIN-A DETERMINATION BY HPLC WITH FLUORESCENCE DETECTION (HPLC-FL): A NEW STANDARDIZATION METHOD FOR WHEAT SAMPLES
title_full	OCHRATOXIN-A DETERMINATION BY HPLC WITH FLUORESCENCE DETECTION (HPLC-FL): A NEW STANDARDIZATION METHOD FOR WHEAT SAMPLES
title_fullStr	OCHRATOXIN-A DETERMINATION BY HPLC WITH FLUORESCENCE DETECTION (HPLC-FL): A NEW STANDARDIZATION METHOD FOR WHEAT SAMPLES
title_full_unstemmed	OCHRATOXIN-A DETERMINATION BY HPLC WITH FLUORESCENCE DETECTION (HPLC-FL): A NEW STANDARDIZATION METHOD FOR WHEAT SAMPLES
title_sort	ochratoxin-a determination by hplc with fluorescence detection (hplc-fl): a new standardization method for wheat samples
publisher	Sociedad Chilena de Nutrición, Bromatología y Toxicología
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-75182010000200007
work_keys_str_mv	AT teixeiramariol ochratoxinadeterminationbyhplcwithfluorescencedetectionhplcflanewstandardizationmethodforwheatsamples AT pertuzzattidaiane ochratoxinadeterminationbyhplcwithfluorescencedetectionhplcflanewstandardizationmethodforwheatsamples AT leitedeborac ochratoxinadeterminationbyhplcwithfluorescencedetectionhplcflanewstandardizationmethodforwheatsamples AT oliveiraluisflavios ochratoxinadeterminationbyhplcwithfluorescencedetectionhplcflanewstandardizationmethodforwheatsamples AT fuentefriaalexandrem ochratoxinadeterminationbyhplcwithfluorescencedetectionhplcflanewstandardizationmethodforwheatsamples
_version_	1718443613142122496

OCHRATOXIN-A DETERMINATION BY HPLC WITH FLUORESCENCE DETECTION (HPLC-FL): A NEW STANDARDIZATION METHOD FOR WHEAT SAMPLES

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