Microtubule cytoskeleton distribution using fluorescent taxoid in Tetratrichomonas didelphidis

Tetratrichomonas didelphidis is a flagellated protozoan found in the intestine of opossums. The specimens were stained by the Giemsa method and by FLUTAX-2, an active fluorescent derivative of Taxol which binds to the ab-tubulin polimerized of microtubules of cells. Giemsa stain revealed the morphol...

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Autores principales:	LECKE,SHEILA B, TASCA,TIANA, SOUTO,ANDRÉ A, BORGES,FERNANDA P, WILTUSCHING,RENATA C. M, DE CARLI,GERALDO A
Lenguaje:	English
Publicado:	Sociedad Chilena de Parasitología 2003
Materias:	Tetratrichomonas didelphidis microtubule cytoskeleton fluorescent taxoid
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000300002
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spelling	oai:scielo:S0717-771220030003000022004-03-12Microtubule cytoskeleton distribution using fluorescent taxoid in Tetratrichomonas didelphidisLECKE,SHEILA BTASCA,TIANASOUTO,ANDRÉ ABORGES,FERNANDA PWILTUSCHING,RENATA C. MDE CARLI,GERALDO A Tetratrichomonas didelphidis microtubule cytoskeleton fluorescent taxoid Tetratrichomonas didelphidis is a flagellated protozoan found in the intestine of opossums. The specimens were stained by the Giemsa method and by FLUTAX-2, an active fluorescent derivative of Taxol which binds to the ab-tubulin polimerized of microtubules of cells. Giemsa stain revealed the morphological features of trichomonads such as four anterior flagella, undulating membrane, axostyle and posterior flagellum. An intense fluorescence was observed in living trophozoites of T. didelphidis and Trichomonas vaginalis (used as control), incubated with FLUTAX-2. An analysis of the composition of the cytoskeleton of T. didelphidis will contribute to understanding the cellular morphology of the parasites.info:eu-repo/semantics/openAccessSociedad Chilena de ParasitologíaParasitología latinoamericana v.58 n.3-4 20032003-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000300002en10.4067/S0717-77122003000300002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Tetratrichomonas didelphidis microtubule cytoskeleton fluorescent taxoid
spellingShingle	Tetratrichomonas didelphidis microtubule cytoskeleton fluorescent taxoid LECKE,SHEILA B TASCA,TIANA SOUTO,ANDRÉ A BORGES,FERNANDA P WILTUSCHING,RENATA C. M DE CARLI,GERALDO A Microtubule cytoskeleton distribution using fluorescent taxoid in Tetratrichomonas didelphidis
description	Tetratrichomonas didelphidis is a flagellated protozoan found in the intestine of opossums. The specimens were stained by the Giemsa method and by FLUTAX-2, an active fluorescent derivative of Taxol which binds to the ab-tubulin polimerized of microtubules of cells. Giemsa stain revealed the morphological features of trichomonads such as four anterior flagella, undulating membrane, axostyle and posterior flagellum. An intense fluorescence was observed in living trophozoites of T. didelphidis and Trichomonas vaginalis (used as control), incubated with FLUTAX-2. An analysis of the composition of the cytoskeleton of T. didelphidis will contribute to understanding the cellular morphology of the parasites.
author	LECKE,SHEILA B TASCA,TIANA SOUTO,ANDRÉ A BORGES,FERNANDA P WILTUSCHING,RENATA C. M DE CARLI,GERALDO A
author_facet	LECKE,SHEILA B TASCA,TIANA SOUTO,ANDRÉ A BORGES,FERNANDA P WILTUSCHING,RENATA C. M DE CARLI,GERALDO A
author_sort	LECKE,SHEILA B
title	Microtubule cytoskeleton distribution using fluorescent taxoid in Tetratrichomonas didelphidis
title_short	Microtubule cytoskeleton distribution using fluorescent taxoid in Tetratrichomonas didelphidis
title_full	Microtubule cytoskeleton distribution using fluorescent taxoid in Tetratrichomonas didelphidis
title_fullStr	Microtubule cytoskeleton distribution using fluorescent taxoid in Tetratrichomonas didelphidis
title_full_unstemmed	Microtubule cytoskeleton distribution using fluorescent taxoid in Tetratrichomonas didelphidis
title_sort	microtubule cytoskeleton distribution using fluorescent taxoid in tetratrichomonas didelphidis
publisher	Sociedad Chilena de Parasitología
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000300002
work_keys_str_mv	AT leckesheilab microtubulecytoskeletondistributionusingfluorescenttaxoidintetratrichomonasdidelphidis AT tascatiana microtubulecytoskeletondistributionusingfluorescenttaxoidintetratrichomonasdidelphidis AT soutoandrea microtubulecytoskeletondistributionusingfluorescenttaxoidintetratrichomonasdidelphidis AT borgesfernandap microtubulecytoskeletondistributionusingfluorescenttaxoidintetratrichomonasdidelphidis AT wiltuschingrenatacm microtubulecytoskeletondistributionusingfluorescenttaxoidintetratrichomonasdidelphidis AT decarligeraldoa microtubulecytoskeletondistributionusingfluorescenttaxoidintetratrichomonasdidelphidis
_version_	1718444077812285440

Microtubule cytoskeleton distribution using fluorescent taxoid in Tetratrichomonas didelphidis

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