Genotyping of Toxoplasma gondii strains detected in pork sausage

Genotyping of Toxoplasma gondii strains detected in pork sausage

DNA was extracted from 70 pork sausage samples obtained from 55 commercial establishments in the city of Botucatu, Sao Paulo, Brazil. Nested-polymerase chain reaction with primers specific for Toxoplasma gondii SAG2 locus was used and detected the parasite in 19 (27.14%) sausage samples. Digestion o...

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Autores principales: VIEIRA DA SILVA,ARISTEU, DE OLIVEIRA MENDONÇA,ANDRÉ, BERGAMASCHI PEZERICO,SANDIA, DOMINGUES,PAULO FRANCISCO, LANGONI,HELIO
Lenguaje:English
Publicado: Sociedad Chilena de Parasitología 2005
Materias:
Toxoplasma gondii
SAG2 genotyping
food-borne pathogen
sausage
swine
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122005000100011
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spelling oai:scielo:S0717-771220050001000112005-07-18Genotyping of Toxoplasma gondii strains detected in pork sausageVIEIRA DA SILVA,ARISTEUDE OLIVEIRA MENDONÇA,ANDRÉBERGAMASCHI PEZERICO,SANDIADOMINGUES,PAULO FRANCISCOLANGONI,HELIO Toxoplasma gondii SAG2 genotyping food-borne pathogen sausage swine DNA was extracted from 70 pork sausage samples obtained from 55 commercial establishments in the city of Botucatu, Sao Paulo, Brazil. Nested-polymerase chain reaction with primers specific for Toxoplasma gondii SAG2 locus was used and detected the parasite in 19 (27.14%) sausage samples. Digestion of amplicons with restriction enzymes HhaI and Sau3AI showed that 14 (73.68%) samples contained parasites genotyped as Type I, and 5 (26.32%) as Type IIIinfo:eu-repo/semantics/openAccessSociedad Chilena de ParasitologíaParasitología latinoamericana v.60 n.1-2 20052005-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122005000100011en10.4067/S0717-77122005000100011
institution Scielo Chile
collection Scielo Chile
language English
topic Toxoplasma gondii
SAG2 genotyping
food-borne pathogen
sausage
swine
spellingShingle Toxoplasma gondii
SAG2 genotyping
food-borne pathogen
sausage
swine
VIEIRA DA SILVA,ARISTEU
DE OLIVEIRA MENDONÇA,ANDRÉ
BERGAMASCHI PEZERICO,SANDIA
DOMINGUES,PAULO FRANCISCO
LANGONI,HELIO
Genotyping of Toxoplasma gondii strains detected in pork sausage
description DNA was extracted from 70 pork sausage samples obtained from 55 commercial establishments in the city of Botucatu, Sao Paulo, Brazil. Nested-polymerase chain reaction with primers specific for Toxoplasma gondii SAG2 locus was used and detected the parasite in 19 (27.14%) sausage samples. Digestion of amplicons with restriction enzymes HhaI and Sau3AI showed that 14 (73.68%) samples contained parasites genotyped as Type I, and 5 (26.32%) as Type III
author VIEIRA DA SILVA,ARISTEU
DE OLIVEIRA MENDONÇA,ANDRÉ
BERGAMASCHI PEZERICO,SANDIA
DOMINGUES,PAULO FRANCISCO
LANGONI,HELIO
author_facet VIEIRA DA SILVA,ARISTEU
DE OLIVEIRA MENDONÇA,ANDRÉ
BERGAMASCHI PEZERICO,SANDIA
DOMINGUES,PAULO FRANCISCO
LANGONI,HELIO
author_sort VIEIRA DA SILVA,ARISTEU
title Genotyping of Toxoplasma gondii strains detected in pork sausage
title_short Genotyping of Toxoplasma gondii strains detected in pork sausage
title_full Genotyping of Toxoplasma gondii strains detected in pork sausage
title_fullStr Genotyping of Toxoplasma gondii strains detected in pork sausage
title_full_unstemmed Genotyping of Toxoplasma gondii strains detected in pork sausage
title_sort genotyping of toxoplasma gondii strains detected in pork sausage
publisher Sociedad Chilena de Parasitología
publishDate 2005
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122005000100011
work_keys_str_mv AT vieiradasilvaaristeu genotypingoftoxoplasmagondiistrainsdetectedinporksausage
AT deoliveiramendoncaandre genotypingoftoxoplasmagondiistrainsdetectedinporksausage
AT bergamaschipezericosandia genotypingoftoxoplasmagondiistrainsdetectedinporksausage
AT dominguespaulofrancisco genotypingoftoxoplasmagondiistrainsdetectedinporksausage
AT langonihelio genotypingoftoxoplasmagondiistrainsdetectedinporksausage
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