Effects of Acute Chagas'Disease on Mice Central Nervous System

This study has been done to evaluate the central nervous system (CNS) of mice infected with Trypanosoma cruzi and its relationships with the irreversible decrease of motor activity of the rear limbs during acute Chagas´disease. The course of the present study shows the in vivo behaviour of three par...

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Autores principales:	LUGO DE YARBUH,ANA, ARAUJO,SONIA, COLASANTE,CESARE, ALARCÓN,MARITZA, MORENO,ELIO
Lenguaje:	English
Publicado:	Sociedad Chilena de Parasitología 2006
Materias:	Brain Spinal Cord Neurons Motoneurons Acute Chagas'disease
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122006000100001
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spelling	oai:scielo:S0717-771220060001000012013-07-10Effects of Acute Chagas'Disease on Mice Central Nervous SystemLUGO DE YARBUH,ANAARAUJO,SONIACOLASANTE,CESAREALARCÓN,MARITZAMORENO,ELIO Brain Spinal Cord Neurons Motoneurons Acute Chagas'disease This study has been done to evaluate the central nervous system (CNS) of mice infected with Trypanosoma cruzi and its relationships with the irreversible decrease of motor activity of the rear limbs during acute Chagas´disease. The course of the present study shows the in vivo behaviour of three parasites strains which were isolated from different sources and geographical areas, with the purpose of explaining the parasitemia, mortality rate, clinical, pathological and histopathological changes in the CNS of infected mice. The mice were injected intraperitoneally with 5.10³ bloodstreams of different T. cruzi strains. The mice infected with PR and ASM strains from Venezuela, showed low parasitemia and high mortality, while the Y strain produced higher parasitemia levels. At the 30th day post-infection both left parietal brain cortex (LPC) and spinal cord (SC) were sectioned, stained with hematoxilin and eosin (H-E) and examined by means of confocal ligth microscopy. At this time, the pathology of the CNS exhibited focal infiltrates of monocytes, lymphocytes, plasmocytes, polymorphonuclear cells and loss of neuronas and motoneurons. The sections of LPC of infected mice with ASM strain, showed loss neuronal, parasites and abundant T. cruzi antigen deposits in the proximity of the swollen neurons. The sections of SC stained with Enolase-Avidin-Biotin-Peroxidase showed a reduction in the average number of neurons of the cervical region (CR) of the infected mice with PR, ASM and Y strains. Sections stained with Propidium Ioduro (IP) showed a reduction of the number of motoneurons in all regions of the SC, with a significant difference between groups infected with different T. cruzi strains and control uninfected mice (P < 0.05). This study established a correlation between the parasitism in the proximity to inflammatory cells, together the appearance of T. cruzi antigen and neuronal destruction in the brain. Therefore it can be concluded that the changes in CNS may be attributed to early parasitism in nervous tissue, which occur in a few days, involving clinico-pathological manifestations, which produced alterations of the mobility with paralysis of the rear limbs and death in 100% of mice with acute infection produced by PR and ASM-T. cruzi strains from Venezuelainfo:eu-repo/semantics/openAccessSociedad Chilena de ParasitologíaParasitología latinoamericana v.61 n.1-2 20062006-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122006000100001en10.4067/S0717-77122006000100001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Brain Spinal Cord Neurons Motoneurons Acute Chagas'disease
spellingShingle	Brain Spinal Cord Neurons Motoneurons Acute Chagas'disease LUGO DE YARBUH,ANA ARAUJO,SONIA COLASANTE,CESARE ALARCÓN,MARITZA MORENO,ELIO Effects of Acute Chagas'Disease on Mice Central Nervous System
description	This study has been done to evaluate the central nervous system (CNS) of mice infected with Trypanosoma cruzi and its relationships with the irreversible decrease of motor activity of the rear limbs during acute Chagas´disease. The course of the present study shows the in vivo behaviour of three parasites strains which were isolated from different sources and geographical areas, with the purpose of explaining the parasitemia, mortality rate, clinical, pathological and histopathological changes in the CNS of infected mice. The mice were injected intraperitoneally with 5.10³ bloodstreams of different T. cruzi strains. The mice infected with PR and ASM strains from Venezuela, showed low parasitemia and high mortality, while the Y strain produced higher parasitemia levels. At the 30th day post-infection both left parietal brain cortex (LPC) and spinal cord (SC) were sectioned, stained with hematoxilin and eosin (H-E) and examined by means of confocal ligth microscopy. At this time, the pathology of the CNS exhibited focal infiltrates of monocytes, lymphocytes, plasmocytes, polymorphonuclear cells and loss of neuronas and motoneurons. The sections of LPC of infected mice with ASM strain, showed loss neuronal, parasites and abundant T. cruzi antigen deposits in the proximity of the swollen neurons. The sections of SC stained with Enolase-Avidin-Biotin-Peroxidase showed a reduction in the average number of neurons of the cervical region (CR) of the infected mice with PR, ASM and Y strains. Sections stained with Propidium Ioduro (IP) showed a reduction of the number of motoneurons in all regions of the SC, with a significant difference between groups infected with different T. cruzi strains and control uninfected mice (P < 0.05). This study established a correlation between the parasitism in the proximity to inflammatory cells, together the appearance of T. cruzi antigen and neuronal destruction in the brain. Therefore it can be concluded that the changes in CNS may be attributed to early parasitism in nervous tissue, which occur in a few days, involving clinico-pathological manifestations, which produced alterations of the mobility with paralysis of the rear limbs and death in 100% of mice with acute infection produced by PR and ASM-T. cruzi strains from Venezuela
author	LUGO DE YARBUH,ANA ARAUJO,SONIA COLASANTE,CESARE ALARCÓN,MARITZA MORENO,ELIO
author_facet	LUGO DE YARBUH,ANA ARAUJO,SONIA COLASANTE,CESARE ALARCÓN,MARITZA MORENO,ELIO
author_sort	LUGO DE YARBUH,ANA
title	Effects of Acute Chagas'Disease on Mice Central Nervous System
title_short	Effects of Acute Chagas'Disease on Mice Central Nervous System
title_full	Effects of Acute Chagas'Disease on Mice Central Nervous System
title_fullStr	Effects of Acute Chagas'Disease on Mice Central Nervous System
title_full_unstemmed	Effects of Acute Chagas'Disease on Mice Central Nervous System
title_sort	effects of acute chagas'disease on mice central nervous system
publisher	Sociedad Chilena de Parasitología
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122006000100001
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Effects of Acute Chagas'Disease on Mice Central Nervous System

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