Entamoeba histolytica and Entamoeba dispar: differentiation by Enzyme-Linked Immunosorbet Assay (ELISA) and its clinical correlation in pediatric patients

Studies were carried out at a mexican pediatric hospital to determine the ratio between the pathogenic species Entamoeba histolytica and non-pathogenic species E. dispar using an enzyme-linked immunosorbent assay (ELISA) to detect the lectin (1 galactose N-acetyl D-galactosamine) of E. histolytica i...

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Autores principales: BERNAL REDONDO,ROSAMARÍA, MARTÍNEZ MÉNDEZ,LAURA G, BAER,GEORGE
Lenguaje:English
Publicado: Sociedad Chilena de Parasitología 2006
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122006000100006
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spelling oai:scielo:S0717-771220060001000062013-07-10Entamoeba histolytica and Entamoeba dispar: differentiation by Enzyme-Linked Immunosorbet Assay (ELISA) and its clinical correlation in pediatric patientsBERNAL REDONDO,ROSAMARÍAMARTÍNEZ MÉNDEZ,LAURA GBAER,GEORGE Entamoeba histolytica/E. dispar Entamoebosis intestinal Clinical laboratory techniques Studies were carried out at a mexican pediatric hospital to determine the ratio between the pathogenic species Entamoeba histolytica and non-pathogenic species E. dispar using an enzyme-linked immunosorbent assay (ELISA) to detect the lectin (1 galactose N-acetyl D-galactosamine) of E. histolytica in feces. A close correlation was noted between the presence of the E. histolytica lectin and clinical symptoms. In the study, amebas were detected by microscopy in 120 children (either E. histolytica or E. dispar). But while almost all (13/14) of the children with E. histolytica had clinical symptoms, dysentery-feces with mocus and blood, diarrhea, cramping abdominal pain, tenesmus rectal, flatulence, vomiting and headache, almost none (1/106) of the children infected with the non-pathogenic ameba E. dispar had signs and symptoms. This suggests that much of the amebiasis diagnoses made in Mexico are, in fact, due to non-pathogenic E. disparinfo:eu-repo/semantics/openAccessSociedad Chilena de ParasitologíaParasitología latinoamericana v.61 n.1-2 20062006-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122006000100006en10.4067/S0717-77122006000100006
institution Scielo Chile
collection Scielo Chile
language English
topic Entamoeba histolytica/E. dispar
Entamoebosis intestinal
Clinical laboratory techniques
spellingShingle Entamoeba histolytica/E. dispar
Entamoebosis intestinal
Clinical laboratory techniques
BERNAL REDONDO,ROSAMARÍA
MARTÍNEZ MÉNDEZ,LAURA G
BAER,GEORGE
Entamoeba histolytica and Entamoeba dispar: differentiation by Enzyme-Linked Immunosorbet Assay (ELISA) and its clinical correlation in pediatric patients
description Studies were carried out at a mexican pediatric hospital to determine the ratio between the pathogenic species Entamoeba histolytica and non-pathogenic species E. dispar using an enzyme-linked immunosorbent assay (ELISA) to detect the lectin (1 galactose N-acetyl D-galactosamine) of E. histolytica in feces. A close correlation was noted between the presence of the E. histolytica lectin and clinical symptoms. In the study, amebas were detected by microscopy in 120 children (either E. histolytica or E. dispar). But while almost all (13/14) of the children with E. histolytica had clinical symptoms, dysentery-feces with mocus and blood, diarrhea, cramping abdominal pain, tenesmus rectal, flatulence, vomiting and headache, almost none (1/106) of the children infected with the non-pathogenic ameba E. dispar had signs and symptoms. This suggests that much of the amebiasis diagnoses made in Mexico are, in fact, due to non-pathogenic E. dispar
author BERNAL REDONDO,ROSAMARÍA
MARTÍNEZ MÉNDEZ,LAURA G
BAER,GEORGE
author_facet BERNAL REDONDO,ROSAMARÍA
MARTÍNEZ MÉNDEZ,LAURA G
BAER,GEORGE
author_sort BERNAL REDONDO,ROSAMARÍA
title Entamoeba histolytica and Entamoeba dispar: differentiation by Enzyme-Linked Immunosorbet Assay (ELISA) and its clinical correlation in pediatric patients
title_short Entamoeba histolytica and Entamoeba dispar: differentiation by Enzyme-Linked Immunosorbet Assay (ELISA) and its clinical correlation in pediatric patients
title_full Entamoeba histolytica and Entamoeba dispar: differentiation by Enzyme-Linked Immunosorbet Assay (ELISA) and its clinical correlation in pediatric patients
title_fullStr Entamoeba histolytica and Entamoeba dispar: differentiation by Enzyme-Linked Immunosorbet Assay (ELISA) and its clinical correlation in pediatric patients
title_full_unstemmed Entamoeba histolytica and Entamoeba dispar: differentiation by Enzyme-Linked Immunosorbet Assay (ELISA) and its clinical correlation in pediatric patients
title_sort entamoeba histolytica and entamoeba dispar: differentiation by enzyme-linked immunosorbet assay (elisa) and its clinical correlation in pediatric patients
publisher Sociedad Chilena de Parasitología
publishDate 2006
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122006000100006
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