Detection of Cryptosporidium oocysts by auramine and Ziehl Neelsen staining methods

Cryptosporidium spp is a common intestinal pathogen of animals and humans. It may have an important economic impact on farms and cause potentially zoonotic infections. Fecal specimens were collected from 331 domestic animals (81 beef cattle, 50 sheep, 100 pigs and 100 dogs) and checked for the prese...

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Autores principales: QUADROS,ROSILÉIA M. DE, MARQUES,SANDRA M. T., AMENDOEIRA,CAMILA R, SOUZA,LARISSA A. DE, AMENDOEIRA,PAULA R, COMPARIN,CARLA C
Lenguaje:English
Publicado: Sociedad Chilena de Parasitología 2006
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122006000200003
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spelling oai:scielo:S0717-771220060002000032007-02-28Detection of Cryptosporidium oocysts by auramine and Ziehl Neelsen staining methodsQUADROS,ROSILÉIA M. DEMARQUES,SANDRA M. T.AMENDOEIRA,CAMILA RSOUZA,LARISSA A. DEAMENDOEIRA,PAULA RCOMPARIN,CARLA C Cryptosporidium domestic animals auramine Ziehl Neelsen method Cryptosporidium spp is a common intestinal pathogen of animals and humans. It may have an important economic impact on farms and cause potentially zoonotic infections. Fecal specimens were collected from 331 domestic animals (81 beef cattle, 50 sheep, 100 pigs and 100 dogs) and checked for the presence of Cryptosporidium oocysts by way of Ziehl Neelsen and auramine staining methods. An overall positivity rate of 7.5% (25/331) was found, with rates of 10% (10/100) among the dogs and 18.5% (15/81) among the beef cattle. The feces of sheep and pigs tested negative. In beef cattle, 15 and 12 positive samples were detected by the auramine and Ziehl Neelsen staining techniques, respectively, with no statistically significant difference between the two methods. In dogs, the same number of positive samples was found by both techniquesinfo:eu-repo/semantics/openAccessSociedad Chilena de ParasitologíaParasitología latinoamericana v.61 n.3-4 20062006-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122006000200003en10.4067/S0717-77122006000200003
institution Scielo Chile
collection Scielo Chile
language English
topic Cryptosporidium
domestic animals
auramine
Ziehl Neelsen method
spellingShingle Cryptosporidium
domestic animals
auramine
Ziehl Neelsen method
QUADROS,ROSILÉIA M. DE
MARQUES,SANDRA M. T.
AMENDOEIRA,CAMILA R
SOUZA,LARISSA A. DE
AMENDOEIRA,PAULA R
COMPARIN,CARLA C
Detection of Cryptosporidium oocysts by auramine and Ziehl Neelsen staining methods
description Cryptosporidium spp is a common intestinal pathogen of animals and humans. It may have an important economic impact on farms and cause potentially zoonotic infections. Fecal specimens were collected from 331 domestic animals (81 beef cattle, 50 sheep, 100 pigs and 100 dogs) and checked for the presence of Cryptosporidium oocysts by way of Ziehl Neelsen and auramine staining methods. An overall positivity rate of 7.5% (25/331) was found, with rates of 10% (10/100) among the dogs and 18.5% (15/81) among the beef cattle. The feces of sheep and pigs tested negative. In beef cattle, 15 and 12 positive samples were detected by the auramine and Ziehl Neelsen staining techniques, respectively, with no statistically significant difference between the two methods. In dogs, the same number of positive samples was found by both techniques
author QUADROS,ROSILÉIA M. DE
MARQUES,SANDRA M. T.
AMENDOEIRA,CAMILA R
SOUZA,LARISSA A. DE
AMENDOEIRA,PAULA R
COMPARIN,CARLA C
author_facet QUADROS,ROSILÉIA M. DE
MARQUES,SANDRA M. T.
AMENDOEIRA,CAMILA R
SOUZA,LARISSA A. DE
AMENDOEIRA,PAULA R
COMPARIN,CARLA C
author_sort QUADROS,ROSILÉIA M. DE
title Detection of Cryptosporidium oocysts by auramine and Ziehl Neelsen staining methods
title_short Detection of Cryptosporidium oocysts by auramine and Ziehl Neelsen staining methods
title_full Detection of Cryptosporidium oocysts by auramine and Ziehl Neelsen staining methods
title_fullStr Detection of Cryptosporidium oocysts by auramine and Ziehl Neelsen staining methods
title_full_unstemmed Detection of Cryptosporidium oocysts by auramine and Ziehl Neelsen staining methods
title_sort detection of cryptosporidium oocysts by auramine and ziehl neelsen staining methods
publisher Sociedad Chilena de Parasitología
publishDate 2006
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122006000200003
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