Partial purification, immunogenicity and putative new localization of a native Leishmania heat shock protein 70

In this work we focused on a recombinant protein, containing approximately 230 aminoacids from the carboxy-terminal extremity of the Leishmania chagasi heat shock protein 70. The heat shock proteins are among the most abundant parasite antigens and conserved proteins in nature, and this family is on...

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Autores principales:	DA SILVA,ELIETE CAVALCANTI, RAYOL,CYNTHIA D, MOURA,PATRICIA M. M. F, ANDRADE,PAULO P
Lenguaje:	English
Publicado:	Sociedad Chilena de Parasitología 2008
Materias:	heat shock proteins immunogenicity direct agglutination assay DAT
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122008000100002
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spelling	oai:scielo:S0717-771220080001000022009-06-25Partial purification, immunogenicity and putative new localization of a native Leishmania heat shock protein 70DA SILVA,ELIETE CAVALCANTIRAYOL,CYNTHIA DMOURA,PATRICIA M. M. FANDRADE,PAULO P heat shock proteins immunogenicity direct agglutination assay DAT In this work we focused on a recombinant protein, containing approximately 230 aminoacids from the carboxy-terminal extremity of the Leishmania chagasi heat shock protein 70. The heat shock proteins are among the most abundant parasite antigens and conserved proteins in nature, and this family is one of the most immunogenic proteins present within pathogenic organisms. The recombinant protein has been partially purified by electroelution and further precipitation in acetone. The electroelution process did not modify its immunological and antigenic properties, as it continued to be recognized by visceral leishmaniasis positive sera and by the immunological system of rabbits during the immunization, both in ELISA and Western blots. The production of polyclonal sera with an antigen concentration that is far from the maximum dose, strengthens the idea that the proteins of this family are highly antigenic and immunogenic. Our results with these polyclonal sera in the Direct Agglutination Assay allow the conclusion that the Leishmania chagasi native heat shock protein 70 is distributed on the surface of the parasite.info:eu-repo/semantics/openAccessSociedad Chilena de ParasitologíaParasitología latinoamericana v.63 n.1-2-3-4 20082008-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122008000100002en10.4067/S0717-77122008000100002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	heat shock proteins immunogenicity direct agglutination assay DAT
spellingShingle	heat shock proteins immunogenicity direct agglutination assay DAT DA SILVA,ELIETE CAVALCANTI RAYOL,CYNTHIA D MOURA,PATRICIA M. M. F ANDRADE,PAULO P Partial purification, immunogenicity and putative new localization of a native Leishmania heat shock protein 70
description	In this work we focused on a recombinant protein, containing approximately 230 aminoacids from the carboxy-terminal extremity of the Leishmania chagasi heat shock protein 70. The heat shock proteins are among the most abundant parasite antigens and conserved proteins in nature, and this family is one of the most immunogenic proteins present within pathogenic organisms. The recombinant protein has been partially purified by electroelution and further precipitation in acetone. The electroelution process did not modify its immunological and antigenic properties, as it continued to be recognized by visceral leishmaniasis positive sera and by the immunological system of rabbits during the immunization, both in ELISA and Western blots. The production of polyclonal sera with an antigen concentration that is far from the maximum dose, strengthens the idea that the proteins of this family are highly antigenic and immunogenic. Our results with these polyclonal sera in the Direct Agglutination Assay allow the conclusion that the Leishmania chagasi native heat shock protein 70 is distributed on the surface of the parasite.
author	DA SILVA,ELIETE CAVALCANTI RAYOL,CYNTHIA D MOURA,PATRICIA M. M. F ANDRADE,PAULO P
author_facet	DA SILVA,ELIETE CAVALCANTI RAYOL,CYNTHIA D MOURA,PATRICIA M. M. F ANDRADE,PAULO P
author_sort	DA SILVA,ELIETE CAVALCANTI
title	Partial purification, immunogenicity and putative new localization of a native Leishmania heat shock protein 70
title_short	Partial purification, immunogenicity and putative new localization of a native Leishmania heat shock protein 70
title_full	Partial purification, immunogenicity and putative new localization of a native Leishmania heat shock protein 70
title_fullStr	Partial purification, immunogenicity and putative new localization of a native Leishmania heat shock protein 70
title_full_unstemmed	Partial purification, immunogenicity and putative new localization of a native Leishmania heat shock protein 70
title_sort	partial purification, immunogenicity and putative new localization of a native leishmania heat shock protein 70
publisher	Sociedad Chilena de Parasitología
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122008000100002
work_keys_str_mv	AT dasilvaelietecavalcanti partialpurificationimmunogenicityandputativenewlocalizationofanativeleishmaniaheatshockprotein70 AT rayolcynthiad partialpurificationimmunogenicityandputativenewlocalizationofanativeleishmaniaheatshockprotein70 AT mourapatriciammf partialpurificationimmunogenicityandputativenewlocalizationofanativeleishmaniaheatshockprotein70 AT andradepaulop partialpurificationimmunogenicityandputativenewlocalizationofanativeleishmaniaheatshockprotein70
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Partial purification, immunogenicity and putative new localization of a native Leishmania heat shock protein 70

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