Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiata

Pinus radiata is a conifer of recognized economic value. Studies at genetic and molecular level to support breeding programs of radiata pine have been conducted with difficulty due to its large and complex genome. The development of molecular markers has allowed performing studies on genetic variati...

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Autores principales:	Moraga-Suazo,Priscila, Hasbún,Rodrigo, Balocchi,Claudio, Valenzuela,Sofía
Lenguaje:	English
Publicado:	Universidad Austral de Chile, Facultad de Ciencias Forestales 2012
Materias:	capillary electrophoresis segregation distortion forest trees
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002012000100011
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spelling	oai:scielo:S0717-920020120001000112012-05-11Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiataMoraga-Suazo,PriscilaHasbún,RodrigoBalocchi,ClaudioValenzuela,Sofía capillary electrophoresis segregation distortion forest trees Pinus radiata is a conifer of recognized economic value. Studies at genetic and molecular level to support breeding programs of radiata pine have been conducted with difficulty due to its large and complex genome. The development of molecular markers has allowed performing studies on genetic variation, identification of clones and construction of linkage maps. In order to increase the genetic molecular markers in P. radiata, two inter simple sequence repeat (ISSR) markers and twelve selective amplifications of microsatellite polymorphic loci (SAMPL) primer combinations were tested, using a first-generation full-sib family of 86 individuals. Polymerase chain reaction (PCR) products were visualized by capillary electrophoresis and polymorphism was detected by presence or absence of a particular fragment in one P. radiata parental. A total of 18 polymorphic fragments were found for two ISSR primers tested, with an average segregation distortion of 33 %. SAMPL markers yielded 85 polymorphic fragments with eight primer combinations, with an average of 22.3 % segregation distortion. Both methodologies showed a good reproducibility, with easy implementation and useful for genetic analysis on P. radiata.info:eu-repo/semantics/openAccessUniversidad Austral de Chile, Facultad de Ciencias ForestalesBosque (Valdivia) v.33 n.1 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002012000100011en10.4067/S0717-92002012000100011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	capillary electrophoresis segregation distortion forest trees
spellingShingle	capillary electrophoresis segregation distortion forest trees Moraga-Suazo,Priscila Hasbún,Rodrigo Balocchi,Claudio Valenzuela,Sofía Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiata
description	Pinus radiata is a conifer of recognized economic value. Studies at genetic and molecular level to support breeding programs of radiata pine have been conducted with difficulty due to its large and complex genome. The development of molecular markers has allowed performing studies on genetic variation, identification of clones and construction of linkage maps. In order to increase the genetic molecular markers in P. radiata, two inter simple sequence repeat (ISSR) markers and twelve selective amplifications of microsatellite polymorphic loci (SAMPL) primer combinations were tested, using a first-generation full-sib family of 86 individuals. Polymerase chain reaction (PCR) products were visualized by capillary electrophoresis and polymorphism was detected by presence or absence of a particular fragment in one P. radiata parental. A total of 18 polymorphic fragments were found for two ISSR primers tested, with an average segregation distortion of 33 %. SAMPL markers yielded 85 polymorphic fragments with eight primer combinations, with an average of 22.3 % segregation distortion. Both methodologies showed a good reproducibility, with easy implementation and useful for genetic analysis on P. radiata.
author	Moraga-Suazo,Priscila Hasbún,Rodrigo Balocchi,Claudio Valenzuela,Sofía
author_facet	Moraga-Suazo,Priscila Hasbún,Rodrigo Balocchi,Claudio Valenzuela,Sofía
author_sort	Moraga-Suazo,Priscila
title	Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiata
title_short	Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiata
title_full	Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiata
title_fullStr	Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiata
title_full_unstemmed	Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiata
title_sort	establishment and optimization of issr and sampl molecular markers as a tool for breeding programs of pinus radiata
publisher	Universidad Austral de Chile, Facultad de Ciencias Forestales
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002012000100011
work_keys_str_mv	AT moragasuazopriscila establishmentandoptimizationofissrandsamplmolecularmarkersasatoolforbreedingprogramsofpinusradiata AT hasbunrodrigo establishmentandoptimizationofissrandsamplmolecularmarkersasatoolforbreedingprogramsofpinusradiata AT balocchiclaudio establishmentandoptimizationofissrandsamplmolecularmarkersasatoolforbreedingprogramsofpinusradiata AT valenzuelasofia establishmentandoptimizationofissrandsamplmolecularmarkersasatoolforbreedingprogramsofpinusradiata
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Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiata

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