Genetic diversity in Guazuma crinita from eleven provenances in the Peruvian Amazon revealed by ISSR markers

Guazuma crinita is an important fast-growing timber tree species widely used in agroforestry systems in the Peruvian Amazon. The objectives of our research were (i) to assess genetic diversity of G. crinita using inter-simple sequence repeat (ISSR) markers and (ii) estimate correlation between genet...

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Autores principales: Tuisima Coral,Lady Laura, Hlásná Cepková,Petra, Lojka,Bohdan, Weber,John C, Alves-Milho,Serafín Filomeno
Lenguaje:English
Publicado: Universidad Austral de Chile, Facultad de Ciencias Forestales 2016
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002016000100007
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spelling oai:scielo:S0717-920020160001000072016-05-17Genetic diversity in Guazuma crinita from eleven provenances in the Peruvian Amazon revealed by ISSR markersTuisima Coral,Lady LauraHlásná Cepková,PetraLojka,BohdanWeber,John CAlves-Milho,Serafín Filomeno inter-simple sequence repeat markers genetic diversity genetic distance gene flow geographic distance Guazuma crinita is an important fast-growing timber tree species widely used in agroforestry systems in the Peruvian Amazon. The objectives of our research were (i) to assess genetic diversity of G. crinita using inter-simple sequence repeat (ISSR) markers and (ii) estimate correlation between genetic and geographic distances among provenances. The sample included 44 genotypes from 11 provenances in the Aguaytia and neighboring Pachitea watersheds in the Peruvian Amazon. Ten ISSR primers amplified a total of 65 bands of which 61 were polymorphic (93.8 %). The range of DNA amplification varied from 260 to 2,200 bp. Among the provenances, Macuya exhibited the highest percentage of polymorphic bands (PPB) with 67.7 %, 0.21 Nei's gene diversity (He) and 0.33 Shannon index (I). Overall genetic differentiation (Gjt) was 0.03, indicating 97 % of genetic diversity within provenances. Gene flow (Nm) was 12.9 alleles per generation. Cluster analysis was not related to geographic origin, suggesting a common gene pool. However a weak positive correlation (r = 0.27, P < 0.05) was found between genetic and geographic distance. This is the first study of genetic diversity and structure of G. crinita. We recommend in situ conservation strategies for populations with high levels of genetic diversity.info:eu-repo/semantics/openAccessUniversidad Austral de Chile, Facultad de Ciencias ForestalesBosque (Valdivia) v.37 n.1 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002016000100007en10.4067/S0717-92002016000100007
institution Scielo Chile
collection Scielo Chile
language English
topic inter-simple sequence repeat markers
genetic diversity
genetic distance
gene flow
geographic distance
spellingShingle inter-simple sequence repeat markers
genetic diversity
genetic distance
gene flow
geographic distance
Tuisima Coral,Lady Laura
Hlásná Cepková,Petra
Lojka,Bohdan
Weber,John C
Alves-Milho,Serafín Filomeno
Genetic diversity in Guazuma crinita from eleven provenances in the Peruvian Amazon revealed by ISSR markers
description Guazuma crinita is an important fast-growing timber tree species widely used in agroforestry systems in the Peruvian Amazon. The objectives of our research were (i) to assess genetic diversity of G. crinita using inter-simple sequence repeat (ISSR) markers and (ii) estimate correlation between genetic and geographic distances among provenances. The sample included 44 genotypes from 11 provenances in the Aguaytia and neighboring Pachitea watersheds in the Peruvian Amazon. Ten ISSR primers amplified a total of 65 bands of which 61 were polymorphic (93.8 %). The range of DNA amplification varied from 260 to 2,200 bp. Among the provenances, Macuya exhibited the highest percentage of polymorphic bands (PPB) with 67.7 %, 0.21 Nei's gene diversity (He) and 0.33 Shannon index (I). Overall genetic differentiation (Gjt) was 0.03, indicating 97 % of genetic diversity within provenances. Gene flow (Nm) was 12.9 alleles per generation. Cluster analysis was not related to geographic origin, suggesting a common gene pool. However a weak positive correlation (r = 0.27, P < 0.05) was found between genetic and geographic distance. This is the first study of genetic diversity and structure of G. crinita. We recommend in situ conservation strategies for populations with high levels of genetic diversity.
author Tuisima Coral,Lady Laura
Hlásná Cepková,Petra
Lojka,Bohdan
Weber,John C
Alves-Milho,Serafín Filomeno
author_facet Tuisima Coral,Lady Laura
Hlásná Cepková,Petra
Lojka,Bohdan
Weber,John C
Alves-Milho,Serafín Filomeno
author_sort Tuisima Coral,Lady Laura
title Genetic diversity in Guazuma crinita from eleven provenances in the Peruvian Amazon revealed by ISSR markers
title_short Genetic diversity in Guazuma crinita from eleven provenances in the Peruvian Amazon revealed by ISSR markers
title_full Genetic diversity in Guazuma crinita from eleven provenances in the Peruvian Amazon revealed by ISSR markers
title_fullStr Genetic diversity in Guazuma crinita from eleven provenances in the Peruvian Amazon revealed by ISSR markers
title_full_unstemmed Genetic diversity in Guazuma crinita from eleven provenances in the Peruvian Amazon revealed by ISSR markers
title_sort genetic diversity in guazuma crinita from eleven provenances in the peruvian amazon revealed by issr markers
publisher Universidad Austral de Chile, Facultad de Ciencias Forestales
publishDate 2016
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002016000100007
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