Searching for molecular insight on hybridization in Nothofagus spp. forests at Lagunas de Epulauquen, Argentina

Lagunas de Epulauquen forests (36°49'S; 71°04'W) have been considered the northernmost population of Nothofagus obliqua in Argentina. Recently, however, its taxonomic status has been questioned due to its morphological, architectural and genetic distinctiveness. The convergence of migrator...

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Autores principales:	Azpilicueta,María M, El Mujtar,Verónica A, Gallo,Leonardo A
Lenguaje:	English
Publicado:	Universidad Austral de Chile, Facultad de Ciencias Forestales 2016
Materias:	molecular systematics southern beeches Patagonia SSR markers hybridization
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002016000300016
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spelling	oai:scielo:S0717-920020160003000162019-10-24Searching for molecular insight on hybridization in Nothofagus spp. forests at Lagunas de Epulauquen, ArgentinaAzpilicueta,María MEl Mujtar,Verónica AGallo,Leonardo A molecular systematics southern beeches Patagonia SSR markers hybridization Lagunas de Epulauquen forests (36°49'S; 71°04'W) have been considered the northernmost population of Nothofagus obliqua in Argentina. Recently, however, its taxonomic status has been questioned due to its morphological, architectural and genetic distinctiveness. The convergence of migratory routes and hybridization with Nothofagus alpina are the two main hypotheses proposed to explain the distinctiveness of this population. Despite its unique characteristics, no evaluation of its putative origin has been carried out. In order to test the hypothesis of hybridization we analyzed nuclear Adh and ITS fragment sequences together with 12 nuclear microsatellites. These nuclear regions were selected based on their previously reported divergence between N. obliqua and N. alpina. The survey was conducted in 12 individuals from Lagunas de Epulauquen, and individuals from N. obliqua (8), N. alpina (1), N. macrocarpa (2), N. glauca (4) and N. leonii (2). By combining a phylogenetic analysis with a principal coordinate analysis of microsatellite data, we showed that Lagunas de Epulauquen individuals were closely related to other N. obliqua individuals, while hybridization with N. alpina was not detected. Our results indicate that hybridization processes could be discarded as the origin of the Lagunas de Epulauquen distinctiveness. The hypothesis of the convergence of two N. obliqua migratory routes at this latitude was reinforced based on detected patterns of genetic diversity. Notwithstanding, its high geographical isolation seems to be also a key evolutionary factor to explain the distinctiveness of this population. Lagunas de Epulauquen population could be therefore included within Argentinean N. obliqua domestication and conservation programs.info:eu-repo/semantics/openAccessUniversidad Austral de Chile, Facultad de Ciencias ForestalesBosque (Valdivia) v.37 n.3 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002016000300016en10.4067/S0717-92002016000300016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	molecular systematics southern beeches Patagonia SSR markers hybridization
spellingShingle	molecular systematics southern beeches Patagonia SSR markers hybridization Azpilicueta,María M El Mujtar,Verónica A Gallo,Leonardo A Searching for molecular insight on hybridization in Nothofagus spp. forests at Lagunas de Epulauquen, Argentina
description	Lagunas de Epulauquen forests (36°49'S; 71°04'W) have been considered the northernmost population of Nothofagus obliqua in Argentina. Recently, however, its taxonomic status has been questioned due to its morphological, architectural and genetic distinctiveness. The convergence of migratory routes and hybridization with Nothofagus alpina are the two main hypotheses proposed to explain the distinctiveness of this population. Despite its unique characteristics, no evaluation of its putative origin has been carried out. In order to test the hypothesis of hybridization we analyzed nuclear Adh and ITS fragment sequences together with 12 nuclear microsatellites. These nuclear regions were selected based on their previously reported divergence between N. obliqua and N. alpina. The survey was conducted in 12 individuals from Lagunas de Epulauquen, and individuals from N. obliqua (8), N. alpina (1), N. macrocarpa (2), N. glauca (4) and N. leonii (2). By combining a phylogenetic analysis with a principal coordinate analysis of microsatellite data, we showed that Lagunas de Epulauquen individuals were closely related to other N. obliqua individuals, while hybridization with N. alpina was not detected. Our results indicate that hybridization processes could be discarded as the origin of the Lagunas de Epulauquen distinctiveness. The hypothesis of the convergence of two N. obliqua migratory routes at this latitude was reinforced based on detected patterns of genetic diversity. Notwithstanding, its high geographical isolation seems to be also a key evolutionary factor to explain the distinctiveness of this population. Lagunas de Epulauquen population could be therefore included within Argentinean N. obliqua domestication and conservation programs.
author	Azpilicueta,María M El Mujtar,Verónica A Gallo,Leonardo A
author_facet	Azpilicueta,María M El Mujtar,Verónica A Gallo,Leonardo A
author_sort	Azpilicueta,María M
title	Searching for molecular insight on hybridization in Nothofagus spp. forests at Lagunas de Epulauquen, Argentina
title_short	Searching for molecular insight on hybridization in Nothofagus spp. forests at Lagunas de Epulauquen, Argentina
title_full	Searching for molecular insight on hybridization in Nothofagus spp. forests at Lagunas de Epulauquen, Argentina
title_fullStr	Searching for molecular insight on hybridization in Nothofagus spp. forests at Lagunas de Epulauquen, Argentina
title_full_unstemmed	Searching for molecular insight on hybridization in Nothofagus spp. forests at Lagunas de Epulauquen, Argentina
title_sort	searching for molecular insight on hybridization in nothofagus spp. forests at lagunas de epulauquen, argentina
publisher	Universidad Austral de Chile, Facultad de Ciencias Forestales
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002016000300016
work_keys_str_mv	AT azpilicuetamariam searchingformolecularinsightonhybridizationinnothofagussppforestsatlagunasdeepulauquenargentina AT elmujtarveronicaa searchingformolecularinsightonhybridizationinnothofagussppforestsatlagunasdeepulauquenargentina AT galloleonardoa searchingformolecularinsightonhybridizationinnothofagussppforestsatlagunasdeepulauquenargentina
_version_	1718444225489534976

Searching for molecular insight on hybridization in Nothofagus spp. forests at Lagunas de Epulauquen, Argentina

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