Active chlorine and charcoal affect the in vitro culture of Bambusa vulgaris

SUMMARY: We evaluated the supplementation of the MS culture medium with active chlorine and activated charcoal for the in vitro culture of Bambusa vulgaris. Lateral shoots were collected from field-cultivated plants to obtain explants, which consisted of nodal segments of 1-2 cm length, containing o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cardoso-Furlan,Fernanda, Gavilan,Natália Helena, Zichner-Zorz,Alex, Oliveira,Leandro Silva de, Konzen,Enéas Ricardo, Ebling-Brondani,Gilvano
Lenguaje:	English
Publicado:	Universidad Austral de Chile, Facultad de Ciencias Forestales 2018
Materias:	bamboo asepsis contamination sprout induction micropropagation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002018000100061
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-92002018000100061
record_format	dspace
spelling	oai:scielo:S0717-920020180001000612018-06-13Active chlorine and charcoal affect the in vitro culture of Bambusa vulgarisCardoso-Furlan,FernandaGavilan,Natália HelenaZichner-Zorz,AlexOliveira,Leandro Silva deKonzen,Enéas RicardoEbling-Brondani,Gilvano bamboo asepsis contamination sprout induction micropropagation SUMMARY: We evaluated the supplementation of the MS culture medium with active chlorine and activated charcoal for the in vitro culture of Bambusa vulgaris. Lateral shoots were collected from field-cultivated plants to obtain explants, which consisted of nodal segments of 1-2 cm length, containing one axillary bud. The MS culture medium was supplemented with the following treatments: E1= 0.004 % v v-1 of active chlorine + 4 g L-1 of charcoal; E2= 0.004 % v v-1 of active chlorine; E3= 4 g L-1 charcoal and E4= control (no active chlorine and no charcoal). The percentage of oxidation, fungal and bacterial contamination, explant establishment, survival, sprout and adventitious rooting were evaluated every 21 d, during 63 d. The use of active chlorine reduced the fungal (28 %) and bacterial (38 %) contamination, being the treatment with the highest percentage of established explants (16 %), survival (68 %) and sprout induction (95 %) after 63 d. After 36 days from the beginning of the multiplication stage, some explants presented spontaneous rooting, without the application of plant growth regulators. An anatomical analysis of sprouts and roots revealed the presence of meristematic zones. Furthermore, it indicated that the emergence of adventitious roots occurred from the nodal region in association with axillary buds. Our results contribute toward the development of more efficient protocols for the in vitro propagation of B. vulgaris, from which we recommend using active chlorine for further assessments.info:eu-repo/semantics/openAccessUniversidad Austral de Chile, Facultad de Ciencias ForestalesBosque (Valdivia) v.39 n.1 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002018000100061en10.4067/S0717-92002018000100061
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bamboo asepsis contamination sprout induction micropropagation
spellingShingle	bamboo asepsis contamination sprout induction micropropagation Cardoso-Furlan,Fernanda Gavilan,Natália Helena Zichner-Zorz,Alex Oliveira,Leandro Silva de Konzen,Enéas Ricardo Ebling-Brondani,Gilvano Active chlorine and charcoal affect the in vitro culture of Bambusa vulgaris
description	SUMMARY: We evaluated the supplementation of the MS culture medium with active chlorine and activated charcoal for the in vitro culture of Bambusa vulgaris. Lateral shoots were collected from field-cultivated plants to obtain explants, which consisted of nodal segments of 1-2 cm length, containing one axillary bud. The MS culture medium was supplemented with the following treatments: E1= 0.004 % v v-1 of active chlorine + 4 g L-1 of charcoal; E2= 0.004 % v v-1 of active chlorine; E3= 4 g L-1 charcoal and E4= control (no active chlorine and no charcoal). The percentage of oxidation, fungal and bacterial contamination, explant establishment, survival, sprout and adventitious rooting were evaluated every 21 d, during 63 d. The use of active chlorine reduced the fungal (28 %) and bacterial (38 %) contamination, being the treatment with the highest percentage of established explants (16 %), survival (68 %) and sprout induction (95 %) after 63 d. After 36 days from the beginning of the multiplication stage, some explants presented spontaneous rooting, without the application of plant growth regulators. An anatomical analysis of sprouts and roots revealed the presence of meristematic zones. Furthermore, it indicated that the emergence of adventitious roots occurred from the nodal region in association with axillary buds. Our results contribute toward the development of more efficient protocols for the in vitro propagation of B. vulgaris, from which we recommend using active chlorine for further assessments.
author	Cardoso-Furlan,Fernanda Gavilan,Natália Helena Zichner-Zorz,Alex Oliveira,Leandro Silva de Konzen,Enéas Ricardo Ebling-Brondani,Gilvano
author_facet	Cardoso-Furlan,Fernanda Gavilan,Natália Helena Zichner-Zorz,Alex Oliveira,Leandro Silva de Konzen,Enéas Ricardo Ebling-Brondani,Gilvano
author_sort	Cardoso-Furlan,Fernanda
title	Active chlorine and charcoal affect the in vitro culture of Bambusa vulgaris
title_short	Active chlorine and charcoal affect the in vitro culture of Bambusa vulgaris
title_full	Active chlorine and charcoal affect the in vitro culture of Bambusa vulgaris
title_fullStr	Active chlorine and charcoal affect the in vitro culture of Bambusa vulgaris
title_full_unstemmed	Active chlorine and charcoal affect the in vitro culture of Bambusa vulgaris
title_sort	active chlorine and charcoal affect the in vitro culture of bambusa vulgaris
publisher	Universidad Austral de Chile, Facultad de Ciencias Forestales
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002018000100061
work_keys_str_mv	AT cardosofurlanfernanda activechlorineandcharcoalaffecttheinvitrocultureofbambusavulgaris AT gavilannataliahelena activechlorineandcharcoalaffecttheinvitrocultureofbambusavulgaris AT zichnerzorzalex activechlorineandcharcoalaffecttheinvitrocultureofbambusavulgaris AT oliveiraleandrosilvade activechlorineandcharcoalaffecttheinvitrocultureofbambusavulgaris AT konzeneneasricardo activechlorineandcharcoalaffecttheinvitrocultureofbambusavulgaris AT eblingbrondanigilvano activechlorineandcharcoalaffecttheinvitrocultureofbambusavulgaris
_version_	1718444238679572480

Active chlorine and charcoal affect the in vitro culture of Bambusa vulgaris

Ejemplares similares