Adjustments in the tetrazolium test methodology for assessing the physiological quality of Jatropha mollissima (Euphorbiaceae)

SUMMARY: Jatropha mollissima is a species of the semiarid region of northeastern Brazil. It is known that individuals of the same species may present differences in seeds viability, thus it is necessary to define methodologies to evaluate the physiological potential of seeds. This study aimed at det...

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Autores principales:	Walter,Letícia Siqueira, Gabira,Mônica Moreno, Almeida da Silva,Marcelle, Nogueira,Antonio C, Kratz,Dagma
Lenguaje:	English
Publicado:	Universidad Austral de Chile, Facultad de Ciencias Forestales 2020
Materias:	caatinga pinhão-bravo seeds analysis viability
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002020000100077
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spelling	oai:scielo:S0717-920020200001000772020-07-10Adjustments in the tetrazolium test methodology for assessing the physiological quality of Jatropha mollissima (Euphorbiaceae)Walter,Letícia SiqueiraGabira,Mônica MorenoAlmeida da Silva,MarcelleNogueira,Antonio CKratz,Dagma caatinga pinhão-bravo seeds analysis viability SUMMARY: Jatropha mollissima is a species of the semiarid region of northeastern Brazil. It is known that individuals of the same species may present differences in seeds viability, thus it is necessary to define methodologies to evaluate the physiological potential of seeds. This study aimed at determining the best pre-moistening time and the concentration of the tetrazolium solution to evaluate the viability of J. mollissima seeds. Three pre-moistening times were tested (12, 14 and 16 hours) and two tetrazolium solution concentrations (0.25 and 0.5 %). As a comparative study, a germination test was performed, which consisted of two treatments: seeds between paper and on vermiculite. The values obtained in the tetrazolium test were similar, with average viable seeds of 21.3% for concentration 0.25 % and 27.3 % for the concentration of 0.5 %. It was observed, in the germination test, that the percentage of germination had averages equal to 22.5 % for the treatment between paper and 13.5 % for the treatment with vermiculite. The best procedure for performing the tetrazolium test in J. mollissima seeds is using the concentration of 0.25 % of tetrazolium solution, pre-moistening time of 16 hours, between paper at 25 ºC and staining for 4 hours at 40 °C in the dark.info:eu-repo/semantics/openAccessUniversidad Austral de Chile, Facultad de Ciencias ForestalesBosque (Valdivia) v.41 n.1 20202020-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002020000100077en10.4067/S0717-92002020000100077
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	caatinga pinhão-bravo seeds analysis viability
spellingShingle	caatinga pinhão-bravo seeds analysis viability Walter,Letícia Siqueira Gabira,Mônica Moreno Almeida da Silva,Marcelle Nogueira,Antonio C Kratz,Dagma Adjustments in the tetrazolium test methodology for assessing the physiological quality of Jatropha mollissima (Euphorbiaceae)
description	SUMMARY: Jatropha mollissima is a species of the semiarid region of northeastern Brazil. It is known that individuals of the same species may present differences in seeds viability, thus it is necessary to define methodologies to evaluate the physiological potential of seeds. This study aimed at determining the best pre-moistening time and the concentration of the tetrazolium solution to evaluate the viability of J. mollissima seeds. Three pre-moistening times were tested (12, 14 and 16 hours) and two tetrazolium solution concentrations (0.25 and 0.5 %). As a comparative study, a germination test was performed, which consisted of two treatments: seeds between paper and on vermiculite. The values obtained in the tetrazolium test were similar, with average viable seeds of 21.3% for concentration 0.25 % and 27.3 % for the concentration of 0.5 %. It was observed, in the germination test, that the percentage of germination had averages equal to 22.5 % for the treatment between paper and 13.5 % for the treatment with vermiculite. The best procedure for performing the tetrazolium test in J. mollissima seeds is using the concentration of 0.25 % of tetrazolium solution, pre-moistening time of 16 hours, between paper at 25 ºC and staining for 4 hours at 40 °C in the dark.
author	Walter,Letícia Siqueira Gabira,Mônica Moreno Almeida da Silva,Marcelle Nogueira,Antonio C Kratz,Dagma
author_facet	Walter,Letícia Siqueira Gabira,Mônica Moreno Almeida da Silva,Marcelle Nogueira,Antonio C Kratz,Dagma
author_sort	Walter,Letícia Siqueira
title	Adjustments in the tetrazolium test methodology for assessing the physiological quality of Jatropha mollissima (Euphorbiaceae)
title_short	Adjustments in the tetrazolium test methodology for assessing the physiological quality of Jatropha mollissima (Euphorbiaceae)
title_full	Adjustments in the tetrazolium test methodology for assessing the physiological quality of Jatropha mollissima (Euphorbiaceae)
title_fullStr	Adjustments in the tetrazolium test methodology for assessing the physiological quality of Jatropha mollissima (Euphorbiaceae)
title_full_unstemmed	Adjustments in the tetrazolium test methodology for assessing the physiological quality of Jatropha mollissima (Euphorbiaceae)
title_sort	adjustments in the tetrazolium test methodology for assessing the physiological quality of jatropha mollissima (euphorbiaceae)
publisher	Universidad Austral de Chile, Facultad de Ciencias Forestales
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002020000100077
work_keys_str_mv	AT walterleticiasiqueira adjustmentsinthetetrazoliumtestmethodologyforassessingthephysiologicalqualityofjatrophamollissimaeuphorbiaceae AT gabiramonicamoreno adjustmentsinthetetrazoliumtestmethodologyforassessingthephysiologicalqualityofjatrophamollissimaeuphorbiaceae AT almeidadasilvamarcelle adjustmentsinthetetrazoliumtestmethodologyforassessingthephysiologicalqualityofjatrophamollissimaeuphorbiaceae AT nogueiraantonioc adjustmentsinthetetrazoliumtestmethodologyforassessingthephysiologicalqualityofjatrophamollissimaeuphorbiaceae AT kratzdagma adjustmentsinthetetrazoliumtestmethodologyforassessingthephysiologicalqualityofjatrophamollissimaeuphorbiaceae
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Adjustments in the tetrazolium test methodology for assessing the physiological quality of Jatropha mollissima (Euphorbiaceae)

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