Microplants production of Eucalyptus cloeziana from indirect organogenesis

SUMMARY: Few studies have focused on the in vitro multiplication methods for Eucalyptus cloeziana. In this work we developed an indirect organogenesis protocol to obtain micropropagated plants. The interactions of plant growth regulators in juvenile tissues were evaluated. Hypocotyl, cotyledon and r...

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Autores principales:	Zichner Zorz,Alex, Tannure Faria,Júlio Cézar, Santana Costa Souza,Denys Matheus, Santos Gonçalves,Douglas, Silva de Oliveira,Leandro, Lopes Da Silva,André Luís, Ferreira Campos,Wellington, Ebling Brondani,Gilvano
Lenguaje:	English
Publicado:	Universidad Austral de Chile, Facultad de Ciencias Forestales 2020
Materias:	adventitious rooting callogenesis indirect regeneration adventitious bud
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002020000200113
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spelling	oai:scielo:S0717-920020200002001132020-10-23Microplants production of Eucalyptus cloeziana from indirect organogenesisZichner Zorz,AlexTannure Faria,Júlio CézarSantana Costa Souza,Denys MatheusSantos Gonçalves,DouglasSilva de Oliveira,LeandroLopes Da Silva,André LuísFerreira Campos,WellingtonEbling Brondani,Gilvano adventitious rooting callogenesis indirect regeneration adventitious bud SUMMARY: Few studies have focused on the in vitro multiplication methods for Eucalyptus cloeziana. In this work we developed an indirect organogenesis protocol to obtain micropropagated plants. The interactions of plant growth regulators in juvenile tissues were evaluated. Hypocotyl, cotyledon and root tissues obtained from in vitro germinated seedlings were subjected to combinations of α-naphthaleneacetic acid (NAA), thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D). In vitro callogenesis, adventitious bud induction, shoot elongation, and ex vitro survival and rooting were evaluated. Callogenic structures were observed in all evaluated tissues. Morphogenic characteristics related to the meristematic center was observed. The tissues that presented the callus mass were subcultured in a regeneration culture medium supplemented with 1.0 mg L-1 of 6-benzylaminopurine (BAP). Only hypocotyl and cotyledon induced adventitious bud and shoot elongation. Hypocotyl subjected to 2.0 mg L-1 TDZ had the highest number of elongated shoots. The two tissues (i.e., hypocotyl and cotyledon) combined with thidiazuron were characterized by the ex vitro survival of microcuttings and by adventitious rooting. Results confirmed tissue culture efficiency for microplants production by indirect organogenesis from hypocotyl and cotyledon cultivated with TDZ, and its implementation can be an alternative for forest tree breeding programs of E. cloeziana.info:eu-repo/semantics/openAccessUniversidad Austral de Chile, Facultad de Ciencias ForestalesBosque (Valdivia) v.41 n.2 20202020-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002020000200113en10.4067/S0717-92002020000200113
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	adventitious rooting callogenesis indirect regeneration adventitious bud
spellingShingle	adventitious rooting callogenesis indirect regeneration adventitious bud Zichner Zorz,Alex Tannure Faria,Júlio Cézar Santana Costa Souza,Denys Matheus Santos Gonçalves,Douglas Silva de Oliveira,Leandro Lopes Da Silva,André Luís Ferreira Campos,Wellington Ebling Brondani,Gilvano Microplants production of Eucalyptus cloeziana from indirect organogenesis
description	SUMMARY: Few studies have focused on the in vitro multiplication methods for Eucalyptus cloeziana. In this work we developed an indirect organogenesis protocol to obtain micropropagated plants. The interactions of plant growth regulators in juvenile tissues were evaluated. Hypocotyl, cotyledon and root tissues obtained from in vitro germinated seedlings were subjected to combinations of α-naphthaleneacetic acid (NAA), thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D). In vitro callogenesis, adventitious bud induction, shoot elongation, and ex vitro survival and rooting were evaluated. Callogenic structures were observed in all evaluated tissues. Morphogenic characteristics related to the meristematic center was observed. The tissues that presented the callus mass were subcultured in a regeneration culture medium supplemented with 1.0 mg L-1 of 6-benzylaminopurine (BAP). Only hypocotyl and cotyledon induced adventitious bud and shoot elongation. Hypocotyl subjected to 2.0 mg L-1 TDZ had the highest number of elongated shoots. The two tissues (i.e., hypocotyl and cotyledon) combined with thidiazuron were characterized by the ex vitro survival of microcuttings and by adventitious rooting. Results confirmed tissue culture efficiency for microplants production by indirect organogenesis from hypocotyl and cotyledon cultivated with TDZ, and its implementation can be an alternative for forest tree breeding programs of E. cloeziana.
author	Zichner Zorz,Alex Tannure Faria,Júlio Cézar Santana Costa Souza,Denys Matheus Santos Gonçalves,Douglas Silva de Oliveira,Leandro Lopes Da Silva,André Luís Ferreira Campos,Wellington Ebling Brondani,Gilvano
author_facet	Zichner Zorz,Alex Tannure Faria,Júlio Cézar Santana Costa Souza,Denys Matheus Santos Gonçalves,Douglas Silva de Oliveira,Leandro Lopes Da Silva,André Luís Ferreira Campos,Wellington Ebling Brondani,Gilvano
author_sort	Zichner Zorz,Alex
title	Microplants production of Eucalyptus cloeziana from indirect organogenesis
title_short	Microplants production of Eucalyptus cloeziana from indirect organogenesis
title_full	Microplants production of Eucalyptus cloeziana from indirect organogenesis
title_fullStr	Microplants production of Eucalyptus cloeziana from indirect organogenesis
title_full_unstemmed	Microplants production of Eucalyptus cloeziana from indirect organogenesis
title_sort	microplants production of eucalyptus cloeziana from indirect organogenesis
publisher	Universidad Austral de Chile, Facultad de Ciencias Forestales
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002020000200113
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Microplants production of Eucalyptus cloeziana from indirect organogenesis

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