ULTRASTRUCTURAL ANALYSIS OF PINEALECTOMY AND LACK OF LIGHT INFLUENCE OVER COLLAGEN IN THE ENDOMETRIUM OF RATS

Some studies have shown that pinealectomized female rats present a large concentration of collagen fibers in the endometrium. The lack of light results in morphologic alterations in the uterine lamina propria and in the muscular layer, reducing thus the uterine weight. Therefore, this work aimed to...

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Autores principales:	Pinto de Medeiros,Juliana, Wanderley Teixeira,Valéria, Aguiar Coelho Teixeira,Álvaro, Baratella Evêncio,Liriane, Evêncio Neto,Joaquim
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2003
Materias:	Melatonin Endometrium Pinealectomy Collagen
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000300008
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spelling	oai:scielo:S0717-950220030003000082003-11-28ULTRASTRUCTURAL ANALYSIS OF PINEALECTOMY AND LACK OF LIGHT INFLUENCE OVER COLLAGEN IN THE ENDOMETRIUM OF RATSPinto de Medeiros,JulianaWanderley Teixeira,ValériaAguiar Coelho Teixeira,ÁlvaroBaratella Evêncio,LirianeEvêncio Neto,Joaquim Melatonin Endometrium Pinealectomy Collagen Some studies have shown that pinealectomized female rats present a large concentration of collagen fibers in the endometrium. The lack of light results in morphologic alterations in the uterine lamina propria and in the muscular layer, reducing thus the uterine weight. Therefore, this work aimed to analyze ultra-structurally the influence of melatonin in the remodeling of the collagen in the endometrium, in the estrus phase, in pinealectomized adult rats or submitted to lack of light. The experimental groups: I- sham-pinealectomized maintained in a clear/dark cycle 12/12 hours, during three months; II- pinealectomized rats maintained in a clear/dark cycle 12/12 hours, for three months; III- normal rats maintained in clear/dark cycle 12/12 hours for three months; IV- normal rats maintained in lack of light, for three months. Fragments of a third of uterus average were gathered, fixed in Karnowsky solution and processed for Transmission Electron Microscope analysis. The results showed that the animals of the group II presented greater incidence of the estrus phase, in a percentile of 60%, and reduction of the proestrus phase. In the groups I, III and IV it had an equivalence of the estrus phase, however the animals of group IV showed a greater incidence of the diestrus phase with an average percentile of 40%. The ultra-structural analysis of the lamina propria of the endometrium in the animals of the groups I and III revealed several fibroblasts with nucleus presenting a little dense chromatin, and cytoplasm contents a great quantity of rugous endoplasmic reticulum. In the animals of the group II was observed a great quantity of fibroblasts, with several developed rugous endoplasmic reticulum, in spite of the great quantity of collagen fibers. In the animals of group IV themselves, few fibroblasts with small quantity of cytoplam that presents a scarce profile of rugous endoplasmic reticulum. Therefore, one can conclude that melatonin may perform a regulator factor in the collagen fibers synthesis by fibroblastsinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.21 n.3 20032003-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000300008en10.4067/S0717-95022003000300008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Melatonin Endometrium Pinealectomy Collagen
spellingShingle	Melatonin Endometrium Pinealectomy Collagen Pinto de Medeiros,Juliana Wanderley Teixeira,Valéria Aguiar Coelho Teixeira,Álvaro Baratella Evêncio,Liriane Evêncio Neto,Joaquim ULTRASTRUCTURAL ANALYSIS OF PINEALECTOMY AND LACK OF LIGHT INFLUENCE OVER COLLAGEN IN THE ENDOMETRIUM OF RATS
description	Some studies have shown that pinealectomized female rats present a large concentration of collagen fibers in the endometrium. The lack of light results in morphologic alterations in the uterine lamina propria and in the muscular layer, reducing thus the uterine weight. Therefore, this work aimed to analyze ultra-structurally the influence of melatonin in the remodeling of the collagen in the endometrium, in the estrus phase, in pinealectomized adult rats or submitted to lack of light. The experimental groups: I- sham-pinealectomized maintained in a clear/dark cycle 12/12 hours, during three months; II- pinealectomized rats maintained in a clear/dark cycle 12/12 hours, for three months; III- normal rats maintained in clear/dark cycle 12/12 hours for three months; IV- normal rats maintained in lack of light, for three months. Fragments of a third of uterus average were gathered, fixed in Karnowsky solution and processed for Transmission Electron Microscope analysis. The results showed that the animals of the group II presented greater incidence of the estrus phase, in a percentile of 60%, and reduction of the proestrus phase. In the groups I, III and IV it had an equivalence of the estrus phase, however the animals of group IV showed a greater incidence of the diestrus phase with an average percentile of 40%. The ultra-structural analysis of the lamina propria of the endometrium in the animals of the groups I and III revealed several fibroblasts with nucleus presenting a little dense chromatin, and cytoplasm contents a great quantity of rugous endoplasmic reticulum. In the animals of the group II was observed a great quantity of fibroblasts, with several developed rugous endoplasmic reticulum, in spite of the great quantity of collagen fibers. In the animals of group IV themselves, few fibroblasts with small quantity of cytoplam that presents a scarce profile of rugous endoplasmic reticulum. Therefore, one can conclude that melatonin may perform a regulator factor in the collagen fibers synthesis by fibroblasts
author	Pinto de Medeiros,Juliana Wanderley Teixeira,Valéria Aguiar Coelho Teixeira,Álvaro Baratella Evêncio,Liriane Evêncio Neto,Joaquim
author_facet	Pinto de Medeiros,Juliana Wanderley Teixeira,Valéria Aguiar Coelho Teixeira,Álvaro Baratella Evêncio,Liriane Evêncio Neto,Joaquim
author_sort	Pinto de Medeiros,Juliana
title	ULTRASTRUCTURAL ANALYSIS OF PINEALECTOMY AND LACK OF LIGHT INFLUENCE OVER COLLAGEN IN THE ENDOMETRIUM OF RATS
title_short	ULTRASTRUCTURAL ANALYSIS OF PINEALECTOMY AND LACK OF LIGHT INFLUENCE OVER COLLAGEN IN THE ENDOMETRIUM OF RATS
title_full	ULTRASTRUCTURAL ANALYSIS OF PINEALECTOMY AND LACK OF LIGHT INFLUENCE OVER COLLAGEN IN THE ENDOMETRIUM OF RATS
title_fullStr	ULTRASTRUCTURAL ANALYSIS OF PINEALECTOMY AND LACK OF LIGHT INFLUENCE OVER COLLAGEN IN THE ENDOMETRIUM OF RATS
title_full_unstemmed	ULTRASTRUCTURAL ANALYSIS OF PINEALECTOMY AND LACK OF LIGHT INFLUENCE OVER COLLAGEN IN THE ENDOMETRIUM OF RATS
title_sort	ultrastructural analysis of pinealectomy and lack of light influence over collagen in the endometrium of rats
publisher	Sociedad Chilena de Anatomía
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000300008
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ULTRASTRUCTURAL ANALYSIS OF PINEALECTOMY AND LACK OF LIGHT INFLUENCE OVER COLLAGEN IN THE ENDOMETRIUM OF RATS

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