HIGH RESOLUTION SEM OF INTRINSIC MUSCLE FIBERS OF ANTERIOR THIRD RET'S TONGUE

The characteristics of intrinsic muscle fibers in the anterior third of the adult rat tongue were studied employing light microscopy, SEM and high-resolution SEM (HRSEM) methods. For light microscopy, specimens were fixed in Bouin's solution and embedded in paraffin. In order to identify the mu...

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Autores principales:	Motoyama,Aracy A., Watanabe,Ii-sei, Ogawa,Koichi
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2003
Materias:	Muscle fibers Tongue Rat HRSEM Mitochondria Basement membrane
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000400006
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id	oai:scielo:S0717-95022003000400006
record_format	dspace
spelling	oai:scielo:S0717-950220030004000062004-04-02HIGH RESOLUTION SEM OF INTRINSIC MUSCLE FIBERS OF ANTERIOR THIRD RET'S TONGUEMotoyama,Aracy A.Watanabe,Ii-seiOgawa,Koichi Muscle fibers Tongue Rat HRSEM Mitochondria Basement membrane The characteristics of intrinsic muscle fibers in the anterior third of the adult rat tongue were studied employing light microscopy, SEM and high-resolution SEM (HRSEM) methods. For light microscopy, specimens were fixed in Bouin's solution and embedded in paraffin. In order to identify the muscle fibers and bundles of collagen fibers, frontal sections were stained with hematoxylin-eosin, azo-carmin and picro-sirius. The results showed that muscle fibers near the lamina propria are fixed with connective tissue, constituting several groups. The bundles of muscle fibers are arranged in longitudinal, vertical and transversal directions. For SEM, specimens were fixed in modified Karnovsky solution and freeze-fractured in liquid nitrogen revealed the collagen fibers and bundles of muscle fibers located in three-dimensional aspects. The samples treated in NaOH solution showed the original location of collagen fibers, constituting complex networks in three-dimensional SEM images. Under HRSEM, fractured specimens in DMSO revealed basement membrane of muscle cell containing sponge-like structures and networks of fine collagen fibrils. Cytoplasmic organelles, such as mitochondria with cristae mitochondriales and sarcoplasmic reticulum, were cleary seen in three-dimensional HRSEM imagesinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.21 n.4 20032003-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000400006en10.4067/S0717-95022003000400006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Muscle fibers Tongue Rat HRSEM Mitochondria Basement membrane
spellingShingle	Muscle fibers Tongue Rat HRSEM Mitochondria Basement membrane Motoyama,Aracy A. Watanabe,Ii-sei Ogawa,Koichi HIGH RESOLUTION SEM OF INTRINSIC MUSCLE FIBERS OF ANTERIOR THIRD RET'S TONGUE
description	The characteristics of intrinsic muscle fibers in the anterior third of the adult rat tongue were studied employing light microscopy, SEM and high-resolution SEM (HRSEM) methods. For light microscopy, specimens were fixed in Bouin's solution and embedded in paraffin. In order to identify the muscle fibers and bundles of collagen fibers, frontal sections were stained with hematoxylin-eosin, azo-carmin and picro-sirius. The results showed that muscle fibers near the lamina propria are fixed with connective tissue, constituting several groups. The bundles of muscle fibers are arranged in longitudinal, vertical and transversal directions. For SEM, specimens were fixed in modified Karnovsky solution and freeze-fractured in liquid nitrogen revealed the collagen fibers and bundles of muscle fibers located in three-dimensional aspects. The samples treated in NaOH solution showed the original location of collagen fibers, constituting complex networks in three-dimensional SEM images. Under HRSEM, fractured specimens in DMSO revealed basement membrane of muscle cell containing sponge-like structures and networks of fine collagen fibrils. Cytoplasmic organelles, such as mitochondria with cristae mitochondriales and sarcoplasmic reticulum, were cleary seen in three-dimensional HRSEM images
author	Motoyama,Aracy A. Watanabe,Ii-sei Ogawa,Koichi
author_facet	Motoyama,Aracy A. Watanabe,Ii-sei Ogawa,Koichi
author_sort	Motoyama,Aracy A.
title	HIGH RESOLUTION SEM OF INTRINSIC MUSCLE FIBERS OF ANTERIOR THIRD RET'S TONGUE
title_short	HIGH RESOLUTION SEM OF INTRINSIC MUSCLE FIBERS OF ANTERIOR THIRD RET'S TONGUE
title_full	HIGH RESOLUTION SEM OF INTRINSIC MUSCLE FIBERS OF ANTERIOR THIRD RET'S TONGUE
title_fullStr	HIGH RESOLUTION SEM OF INTRINSIC MUSCLE FIBERS OF ANTERIOR THIRD RET'S TONGUE
title_full_unstemmed	HIGH RESOLUTION SEM OF INTRINSIC MUSCLE FIBERS OF ANTERIOR THIRD RET'S TONGUE
title_sort	high resolution sem of intrinsic muscle fibers of anterior third ret's tongue
publisher	Sociedad Chilena de Anatomía
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000400006
work_keys_str_mv	AT motoyamaaracya highresolutionsemofintrinsicmusclefibersofanteriorthirdretstongue AT watanabeiisei highresolutionsemofintrinsicmusclefibersofanteriorthirdretstongue AT ogawakoichi highresolutionsemofintrinsicmusclefibersofanteriorthirdretstongue
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HIGH RESOLUTION SEM OF INTRINSIC MUSCLE FIBERS OF ANTERIOR THIRD RET'S TONGUE

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