AGEING INDUCES APOPTOSIS AND INCREASES HSP70 STRESS PROTEIN IN THE EPIDIDYMIS OF Octodon degus

Apoptosis has been largely analyzed in the testis. Nevertheless, the epididymis has been scarcely studied. We analyzed the number of apoptotic cells in the different regions (caput, corpus and cauda) of the epididymis of the South American rodent Octodon degus both young and senile. Apoptosis was id...

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Autores principales:	Bustos-Obregón,Eduardo, Esponda,Pedro
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2004
Materias:	Androgens Apoptosis Epididymis Ageing Heat Shock Proteins
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000100005
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spelling	oai:scielo:S0717-950220040001000052005-10-12AGEING INDUCES APOPTOSIS AND INCREASES HSP70 STRESS PROTEIN IN THE EPIDIDYMIS OF Octodon degusBustos-Obregón,EduardoEsponda,Pedro Androgens Apoptosis Epididymis Ageing Heat Shock Proteins Apoptosis has been largely analyzed in the testis. Nevertheless, the epididymis has been scarcely studied. We analyzed the number of apoptotic cells in the different regions (caput, corpus and cauda) of the epididymis of the South American rodent Octodon degus both young and senile. Apoptosis was identified using the TUNEL method which detects in situ DNA fragmentation. Apoptosis was detected in the principal cells of the epididymal epithelium. The caput epididymis was the region more affected. The caput of young animals showed that 0.32/1000 cells were apoptotic in contrast to 5.1/1000 of senile animals. Also in the cauda epididymis apoptosis is increased with age, appearing 0.14/1000 and 3.9/1000 in young and senile animals, respectively. On the other hand, we used a immunocytochemical method to localize the stress protein HSP70. HSP70 appeared notably increased in the principal cells of the cauda epididymis of senile animals. Changes in the epididymal epithelium are probably due to the low androgen levels existing in senile animals and are a region dependent phenomenoninfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.22 n.1 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000100005en10.4067/S0717-95022004000100005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Androgens Apoptosis Epididymis Ageing Heat Shock Proteins
spellingShingle	Androgens Apoptosis Epididymis Ageing Heat Shock Proteins Bustos-Obregón,Eduardo Esponda,Pedro AGEING INDUCES APOPTOSIS AND INCREASES HSP70 STRESS PROTEIN IN THE EPIDIDYMIS OF Octodon degus
description	Apoptosis has been largely analyzed in the testis. Nevertheless, the epididymis has been scarcely studied. We analyzed the number of apoptotic cells in the different regions (caput, corpus and cauda) of the epididymis of the South American rodent Octodon degus both young and senile. Apoptosis was identified using the TUNEL method which detects in situ DNA fragmentation. Apoptosis was detected in the principal cells of the epididymal epithelium. The caput epididymis was the region more affected. The caput of young animals showed that 0.32/1000 cells were apoptotic in contrast to 5.1/1000 of senile animals. Also in the cauda epididymis apoptosis is increased with age, appearing 0.14/1000 and 3.9/1000 in young and senile animals, respectively. On the other hand, we used a immunocytochemical method to localize the stress protein HSP70. HSP70 appeared notably increased in the principal cells of the cauda epididymis of senile animals. Changes in the epididymal epithelium are probably due to the low androgen levels existing in senile animals and are a region dependent phenomenon
author	Bustos-Obregón,Eduardo Esponda,Pedro
author_facet	Bustos-Obregón,Eduardo Esponda,Pedro
author_sort	Bustos-Obregón,Eduardo
title	AGEING INDUCES APOPTOSIS AND INCREASES HSP70 STRESS PROTEIN IN THE EPIDIDYMIS OF Octodon degus
title_short	AGEING INDUCES APOPTOSIS AND INCREASES HSP70 STRESS PROTEIN IN THE EPIDIDYMIS OF Octodon degus
title_full	AGEING INDUCES APOPTOSIS AND INCREASES HSP70 STRESS PROTEIN IN THE EPIDIDYMIS OF Octodon degus
title_fullStr	AGEING INDUCES APOPTOSIS AND INCREASES HSP70 STRESS PROTEIN IN THE EPIDIDYMIS OF Octodon degus
title_full_unstemmed	AGEING INDUCES APOPTOSIS AND INCREASES HSP70 STRESS PROTEIN IN THE EPIDIDYMIS OF Octodon degus
title_sort	ageing induces apoptosis and increases hsp70 stress protein in the epididymis of octodon degus
publisher	Sociedad Chilena de Anatomía
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000100005
work_keys_str_mv	AT bustosobregoneduardo ageinginducesapoptosisandincreaseshsp70stressproteinintheepididymisofoctodondegus AT espondapedro ageinginducesapoptosisandincreaseshsp70stressproteinintheepididymisofoctodondegus
_version_	1718444553919266816

AGEING INDUCES APOPTOSIS AND INCREASES HSP70 STRESS PROTEIN IN THE EPIDIDYMIS OF Octodon degus

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