Morphometric and High Resolution Scanning Electron Microscopy Study of Calomys callosus Major Palatine Nerve

The characteristics of Calomys callosus major palatine nerve were studied employing light, transmission and high resolution scanning electron microscopy methods. For light microscopy, the specimens were fixed in Bouin's fixative solution, processed routinely and the sections were stained with h...

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Autores principales: Tanisaki,Maurício, Ogawa,Koichi, Lapa,Rita de Cássia Ribeiro da Silva, da Silva,Marcelo Cavenaghi Pereira, Watanabe,Ii-sei
Lenguaje:English
Publicado: Sociedad Chilena de Anatomía 2005
Materias:
TEM
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000100003
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spelling oai:scielo:S0717-950220050001000032005-05-17Morphometric and High Resolution Scanning Electron Microscopy Study of Calomys callosus Major Palatine NerveTanisaki,MaurícioOgawa,KoichiLapa,Rita de Cássia Ribeiro da Silvada Silva,Marcelo Cavenaghi PereiraWatanabe,Ii-sei Major palatine nerve HRSEM TEM Calomys callosus Myelin sheath Morphometric The characteristics of Calomys callosus major palatine nerve were studied employing light, transmission and high resolution scanning electron microscopy methods. For light microscopy, the specimens were fixed in Bouin's fixative solution, processed routinely and the sections were stained with hematoxylin­eosin and Azo-Carmin to identify nerve fibers. For high resolution scanning electron microscopy the O-D-O method reported by <a href="#13">Tanaka (1989)</a> was used to examine nerve fiber components and to measure the myelin sheath. Thin sections were examined by transmission electron microscopy to show axoplasmic elements and adjacent structures. The results revealed nerve fiber bundles in the lamina propria of Calomys callosus palatine mucosa. Nerve fibers were enveloped by cytoplasmic lamellae of perineural cells and adjacent collagen bundles, their diameter ranged from 1 to 6 µm, and the myelin sheath ranged from 0.2 to 0.9 µm. In the nerve fibers axoplasm were seen neurofilaments, mitochondria, neurotubules and few unmyelinated fibersinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.23 n.1 20052005-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000100003en10.4067/S0717-95022005000100003
institution Scielo Chile
collection Scielo Chile
language English
topic Major palatine nerve
HRSEM
TEM
Calomys callosus
Myelin sheath
Morphometric
spellingShingle Major palatine nerve
HRSEM
TEM
Calomys callosus
Myelin sheath
Morphometric
Tanisaki,Maurício
Ogawa,Koichi
Lapa,Rita de Cássia Ribeiro da Silva
da Silva,Marcelo Cavenaghi Pereira
Watanabe,Ii-sei
Morphometric and High Resolution Scanning Electron Microscopy Study of Calomys callosus Major Palatine Nerve
description The characteristics of Calomys callosus major palatine nerve were studied employing light, transmission and high resolution scanning electron microscopy methods. For light microscopy, the specimens were fixed in Bouin's fixative solution, processed routinely and the sections were stained with hematoxylin­eosin and Azo-Carmin to identify nerve fibers. For high resolution scanning electron microscopy the O-D-O method reported by <a href="#13">Tanaka (1989)</a> was used to examine nerve fiber components and to measure the myelin sheath. Thin sections were examined by transmission electron microscopy to show axoplasmic elements and adjacent structures. The results revealed nerve fiber bundles in the lamina propria of Calomys callosus palatine mucosa. Nerve fibers were enveloped by cytoplasmic lamellae of perineural cells and adjacent collagen bundles, their diameter ranged from 1 to 6 µm, and the myelin sheath ranged from 0.2 to 0.9 µm. In the nerve fibers axoplasm were seen neurofilaments, mitochondria, neurotubules and few unmyelinated fibers
author Tanisaki,Maurício
Ogawa,Koichi
Lapa,Rita de Cássia Ribeiro da Silva
da Silva,Marcelo Cavenaghi Pereira
Watanabe,Ii-sei
author_facet Tanisaki,Maurício
Ogawa,Koichi
Lapa,Rita de Cássia Ribeiro da Silva
da Silva,Marcelo Cavenaghi Pereira
Watanabe,Ii-sei
author_sort Tanisaki,Maurício
title Morphometric and High Resolution Scanning Electron Microscopy Study of Calomys callosus Major Palatine Nerve
title_short Morphometric and High Resolution Scanning Electron Microscopy Study of Calomys callosus Major Palatine Nerve
title_full Morphometric and High Resolution Scanning Electron Microscopy Study of Calomys callosus Major Palatine Nerve
title_fullStr Morphometric and High Resolution Scanning Electron Microscopy Study of Calomys callosus Major Palatine Nerve
title_full_unstemmed Morphometric and High Resolution Scanning Electron Microscopy Study of Calomys callosus Major Palatine Nerve
title_sort morphometric and high resolution scanning electron microscopy study of calomys callosus major palatine nerve
publisher Sociedad Chilena de Anatomía
publishDate 2005
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000100003
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AT laparitadecassiaribeirodasilva morphometricandhighresolutionscanningelectronmicroscopystudyofcalomyscallosusmajorpalatinenerve
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