Effect of Sodium Cyclamate on the Rat Fetal Liver: A Karyometric and Stereological Study

The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate fetal hepatic karyometric and stereological alteratio...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Martins,Alex Tadeu, Azoubel,Reinaldo, Lopes,Ruberval A., di Matteo,Miguel A. Sala, de Arruda,José Germano Ferraz
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2005
Materias:	Sodium cyclamate Fetal liver Karyometry Stereology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000300005
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022005000300005
record_format	dspace
spelling	oai:scielo:S0717-950220050003000052005-10-28Effect of Sodium Cyclamate on the Rat Fetal Liver: A Karyometric and Stereological StudyMartins,Alex TadeuAzoubel,ReinaldoLopes,Ruberval A.di Matteo,Miguel A. Salade Arruda,José Germano Ferraz Sodium cyclamate Fetal liver Karyometry Stereology The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate fetal hepatic karyometric and stereological alterations in the rat fetal liver resulting from the intraperitoneal administration of sodium cyclamate. The livers of ten rats were evaluated, five treated and five controls chosen at random, in which five rats that received from the 10th to 14th days of pregnancy an intraperitoneal daily injection of sodium cyclamate at 60 mg/Kg of body weight during 5 days. At the 20th day of gestation, the animals were removed and weighed, as were their placentas, on a precision balance; the length of the umbilical cords also were measured. After the laboratory processing, semi-seriated 6mm cuts stained with haematoxyline and eosine were performed. In seven karyometric parameters (major, minor, and medium diameters, volume, area, perimeter, and volume-area ratio), the increase was statistically significant in the treated group when compared with control group. Stereological parameters showed in the treated group a significant increase in the cytoplasmatic and cellular volume, and a significant reduction in the nucleus-cytoplasm ratio as well as in the numerical cellular density. These results showed that the sodium cyclamate in pregnant rats led to retardation of fetal development and hepatic-cellular hypertrophy in the offspring, suggesting toxicity in liver of rat fetusesinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.23 n.3 20052005-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000300005en10.4067/S0717-95022005000300005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Sodium cyclamate Fetal liver Karyometry Stereology
spellingShingle	Sodium cyclamate Fetal liver Karyometry Stereology Martins,Alex Tadeu Azoubel,Reinaldo Lopes,Ruberval A. di Matteo,Miguel A. Sala de Arruda,José Germano Ferraz Effect of Sodium Cyclamate on the Rat Fetal Liver: A Karyometric and Stereological Study
description	The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate fetal hepatic karyometric and stereological alterations in the rat fetal liver resulting from the intraperitoneal administration of sodium cyclamate. The livers of ten rats were evaluated, five treated and five controls chosen at random, in which five rats that received from the 10th to 14th days of pregnancy an intraperitoneal daily injection of sodium cyclamate at 60 mg/Kg of body weight during 5 days. At the 20th day of gestation, the animals were removed and weighed, as were their placentas, on a precision balance; the length of the umbilical cords also were measured. After the laboratory processing, semi-seriated 6mm cuts stained with haematoxyline and eosine were performed. In seven karyometric parameters (major, minor, and medium diameters, volume, area, perimeter, and volume-area ratio), the increase was statistically significant in the treated group when compared with control group. Stereological parameters showed in the treated group a significant increase in the cytoplasmatic and cellular volume, and a significant reduction in the nucleus-cytoplasm ratio as well as in the numerical cellular density. These results showed that the sodium cyclamate in pregnant rats led to retardation of fetal development and hepatic-cellular hypertrophy in the offspring, suggesting toxicity in liver of rat fetuses
author	Martins,Alex Tadeu Azoubel,Reinaldo Lopes,Ruberval A. di Matteo,Miguel A. Sala de Arruda,José Germano Ferraz
author_facet	Martins,Alex Tadeu Azoubel,Reinaldo Lopes,Ruberval A. di Matteo,Miguel A. Sala de Arruda,José Germano Ferraz
author_sort	Martins,Alex Tadeu
title	Effect of Sodium Cyclamate on the Rat Fetal Liver: A Karyometric and Stereological Study
title_short	Effect of Sodium Cyclamate on the Rat Fetal Liver: A Karyometric and Stereological Study
title_full	Effect of Sodium Cyclamate on the Rat Fetal Liver: A Karyometric and Stereological Study
title_fullStr	Effect of Sodium Cyclamate on the Rat Fetal Liver: A Karyometric and Stereological Study
title_full_unstemmed	Effect of Sodium Cyclamate on the Rat Fetal Liver: A Karyometric and Stereological Study
title_sort	effect of sodium cyclamate on the rat fetal liver: a karyometric and stereological study
publisher	Sociedad Chilena de Anatomía
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000300005
work_keys_str_mv	AT martinsalextadeu effectofsodiumcyclamateontheratfetalliverakaryometricandstereologicalstudy AT azoubelreinaldo effectofsodiumcyclamateontheratfetalliverakaryometricandstereologicalstudy AT lopesrubervala effectofsodiumcyclamateontheratfetalliverakaryometricandstereologicalstudy AT dimatteomiguelasala effectofsodiumcyclamateontheratfetalliverakaryometricandstereologicalstudy AT dearrudajosegermanoferraz effectofsodiumcyclamateontheratfetalliverakaryometricandstereologicalstudy
_version_	1718444567220453376

Effect of Sodium Cyclamate on the Rat Fetal Liver: A Karyometric and Stereological Study

Ejemplares similares