Transfection of Gametes: A Method to Generate Transgenic Animals

The production of transgenic animals (TA) using transfected spermatozoa or eggs is commented. Different methods have been employed to introduce transgenes into the gametes of several vertebrates and invertebrates. Methods for the transfection of gametes have employed naked DNA, viral vectors, DNA/Li...

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Autor principal:	Esponda,Pedro
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2005
Materias:	Eggs Oocyte Spermatozoa Transfection Transgenic animals
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000300017
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spelling	oai:scielo:S0717-950220050003000172005-10-28Transfection of Gametes: A Method to Generate Transgenic AnimalsEsponda,Pedro Eggs Oocyte Spermatozoa Transfection Transgenic animals The production of transgenic animals (TA) using transfected spermatozoa or eggs is commented. Different methods have been employed to introduce transgenes into the gametes of several vertebrates and invertebrates. Methods for the transfection of gametes have employed naked DNA, viral vectors, DNA/Liposome complexes, electroporation or high velocity microprojectiles. Spermatozoa and oocytes or eggs have showed a good transfection efficience (80% in some cases), and microscopical observations demonstrated that the transgenes appeared localized in the nucleus. Gametes have shown to be naturally protected against the entrance of foreign genes because some semino plasma or plasma membrane proteins block the entrance of foreign genes in spermatozoa. In the female this blockage is undertaken by the egg covers (the zona pellucida in mammals and the perivitelline coat in mollusks). In several cases the production of TA has been described after using the transfected gametes for in vitro fertilization or inseminations. Sometimes, larger percentages of TA were observed (85% in salmon). Nevertheless, these TA were mainly chimeric for the transgene and they were not capable to establish transgenic lines. It seems probable that TA produced by gamete transfections are different from those originated by the conventional microinjection procedures. Furthermore, gametes would develop some kind of mechanisms that modify the integration/expression of transgenes, or that block the integration of transgenes in the germinal lineinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.23 n.3 20052005-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000300017en10.4067/S0717-95022005000300017
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Eggs Oocyte Spermatozoa Transfection Transgenic animals
spellingShingle	Eggs Oocyte Spermatozoa Transfection Transgenic animals Esponda,Pedro Transfection of Gametes: A Method to Generate Transgenic Animals
description	The production of transgenic animals (TA) using transfected spermatozoa or eggs is commented. Different methods have been employed to introduce transgenes into the gametes of several vertebrates and invertebrates. Methods for the transfection of gametes have employed naked DNA, viral vectors, DNA/Liposome complexes, electroporation or high velocity microprojectiles. Spermatozoa and oocytes or eggs have showed a good transfection efficience (80% in some cases), and microscopical observations demonstrated that the transgenes appeared localized in the nucleus. Gametes have shown to be naturally protected against the entrance of foreign genes because some semino plasma or plasma membrane proteins block the entrance of foreign genes in spermatozoa. In the female this blockage is undertaken by the egg covers (the zona pellucida in mammals and the perivitelline coat in mollusks). In several cases the production of TA has been described after using the transfected gametes for in vitro fertilization or inseminations. Sometimes, larger percentages of TA were observed (85% in salmon). Nevertheless, these TA were mainly chimeric for the transgene and they were not capable to establish transgenic lines. It seems probable that TA produced by gamete transfections are different from those originated by the conventional microinjection procedures. Furthermore, gametes would develop some kind of mechanisms that modify the integration/expression of transgenes, or that block the integration of transgenes in the germinal line
author	Esponda,Pedro
author_facet	Esponda,Pedro
author_sort	Esponda,Pedro
title	Transfection of Gametes: A Method to Generate Transgenic Animals
title_short	Transfection of Gametes: A Method to Generate Transgenic Animals
title_full	Transfection of Gametes: A Method to Generate Transgenic Animals
title_fullStr	Transfection of Gametes: A Method to Generate Transgenic Animals
title_full_unstemmed	Transfection of Gametes: A Method to Generate Transgenic Animals
title_sort	transfection of gametes: a method to generate transgenic animals
publisher	Sociedad Chilena de Anatomía
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000300017
work_keys_str_mv	AT espondapedro transfectionofgametesamethodtogeneratetransgenicanimals
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Transfection of Gametes: A Method to Generate Transgenic Animals

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