Morphometric Analysis of Granulomas Induced by Mycobacterium bovis Suggests an Influence of IFN-Gamma on the Generation and Modulation upon Granulomatous Inflammatory Response in the Different Tissues

There is evidence in both human and experimental infection caused by Mycobacterium tuberculosis, that immunologic factors influence susceptibility to infection and the progress of the disease. The present study aims to evaluate the role of IFN- g upon inflammatory granulomatous response against M. b...

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Autores principales:	Azevedo Duarte,Tonya, Almeida Barbosa Jr.,Aryon de, Arruda,Sergio
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2005
Materias:	Morphometry Mycobacterium Tuberculosis IFN-g Granuloma
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000400006
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spelling	oai:scielo:S0717-950220050004000062006-01-19Morphometric Analysis of Granulomas Induced by Mycobacterium bovis Suggests an Influence of IFN-Gamma on the Generation and Modulation upon Granulomatous Inflammatory Response in the Different TissuesAzevedo Duarte,TonyaAlmeida Barbosa Jr.,Aryon deArruda,Sergio Morphometry Mycobacterium Tuberculosis IFN-g Granuloma There is evidence in both human and experimental infection caused by Mycobacterium tuberculosis, that immunologic factors influence susceptibility to infection and the progress of the disease. The present study aims to evaluate the role of IFN- g upon inflammatory granulomatous response against M. bovis. To pursue that, C57BL/6 mice lacking the genes for synthesis of IFN- g (IFN- g-/-) and their wild-type counterparts (IFN- g+/+) were intravenously inoculated with M. bovis. The ability of M. bovis to survive and replicate in the liver and lungs was evaluated by counting colony-forming unit (CFU). The histopathological features of granulomatous inflammatory response in the liver and lungs were analyzed during the infection by M. bovis. Granuloma parameters such as, size (sectional area), granuloma volume, volume density, and numerical density were calculated in each point of infection. Bacillary load was higher in both organs of the animals that were IFN- g-/- than in IFN- g+/+ mice. Granulomas were observed in the IFN- g-/- mice after 30 days of infection and were detected earlier in controls (15 days of infection). Hepatic granulomas persisted in the IFN- g-/- mice, but in the IFN- g+/+ mice control of the inflammation. In conclusion, IFN- g influenced the multiplication of M. bovis, as well as modulated the granulomatous inflammationinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.23 n.4 20052005-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000400006en10.4067/S0717-95022005000400006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Morphometry Mycobacterium Tuberculosis IFN-g Granuloma
spellingShingle	Morphometry Mycobacterium Tuberculosis IFN-g Granuloma Azevedo Duarte,Tonya Almeida Barbosa Jr.,Aryon de Arruda,Sergio Morphometric Analysis of Granulomas Induced by Mycobacterium bovis Suggests an Influence of IFN-Gamma on the Generation and Modulation upon Granulomatous Inflammatory Response in the Different Tissues
description	There is evidence in both human and experimental infection caused by Mycobacterium tuberculosis, that immunologic factors influence susceptibility to infection and the progress of the disease. The present study aims to evaluate the role of IFN- g upon inflammatory granulomatous response against M. bovis. To pursue that, C57BL/6 mice lacking the genes for synthesis of IFN- g (IFN- g-/-) and their wild-type counterparts (IFN- g+/+) were intravenously inoculated with M. bovis. The ability of M. bovis to survive and replicate in the liver and lungs was evaluated by counting colony-forming unit (CFU). The histopathological features of granulomatous inflammatory response in the liver and lungs were analyzed during the infection by M. bovis. Granuloma parameters such as, size (sectional area), granuloma volume, volume density, and numerical density were calculated in each point of infection. Bacillary load was higher in both organs of the animals that were IFN- g-/- than in IFN- g+/+ mice. Granulomas were observed in the IFN- g-/- mice after 30 days of infection and were detected earlier in controls (15 days of infection). Hepatic granulomas persisted in the IFN- g-/- mice, but in the IFN- g+/+ mice control of the inflammation. In conclusion, IFN- g influenced the multiplication of M. bovis, as well as modulated the granulomatous inflammation
author	Azevedo Duarte,Tonya Almeida Barbosa Jr.,Aryon de Arruda,Sergio
author_facet	Azevedo Duarte,Tonya Almeida Barbosa Jr.,Aryon de Arruda,Sergio
author_sort	Azevedo Duarte,Tonya
title	Morphometric Analysis of Granulomas Induced by Mycobacterium bovis Suggests an Influence of IFN-Gamma on the Generation and Modulation upon Granulomatous Inflammatory Response in the Different Tissues
title_short	Morphometric Analysis of Granulomas Induced by Mycobacterium bovis Suggests an Influence of IFN-Gamma on the Generation and Modulation upon Granulomatous Inflammatory Response in the Different Tissues
title_full	Morphometric Analysis of Granulomas Induced by Mycobacterium bovis Suggests an Influence of IFN-Gamma on the Generation and Modulation upon Granulomatous Inflammatory Response in the Different Tissues
title_fullStr	Morphometric Analysis of Granulomas Induced by Mycobacterium bovis Suggests an Influence of IFN-Gamma on the Generation and Modulation upon Granulomatous Inflammatory Response in the Different Tissues
title_full_unstemmed	Morphometric Analysis of Granulomas Induced by Mycobacterium bovis Suggests an Influence of IFN-Gamma on the Generation and Modulation upon Granulomatous Inflammatory Response in the Different Tissues
title_sort	morphometric analysis of granulomas induced by mycobacterium bovis suggests an influence of ifn-gamma on the generation and modulation upon granulomatous inflammatory response in the different tissues
publisher	Sociedad Chilena de Anatomía
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000400006
work_keys_str_mv	AT azevedoduartetonya morphometricanalysisofgranulomasinducedbymycobacteriumbovissuggestsaninfluenceofifngammaonthegenerationandmodulationupongranulomatousinflammatoryresponseinthedifferenttissues AT almeidabarbosajraryonde morphometricanalysisofgranulomasinducedbymycobacteriumbovissuggestsaninfluenceofifngammaonthegenerationandmodulationupongranulomatousinflammatoryresponseinthedifferenttissues AT arrudasergio morphometricanalysisofgranulomasinducedbymycobacteriumbovissuggestsaninfluenceofifngammaonthegenerationandmodulationupongranulomatousinflammatoryresponseinthedifferenttissues
_version_	1718444570410221568

Morphometric Analysis of Granulomas Induced by Mycobacterium bovis Suggests an Influence of IFN-Gamma on the Generation and Modulation upon Granulomatous Inflammatory Response in the Different Tissues

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