Ultrastructural Aspects of Spermatids in Isogenic Black Mouse C57BL6J

The objective of this study was to describe some changes in spermatids differentiating events during spermiogenesis, leading to spermatozoa final formation. The animals were sacrificed with ethylic acid inhalation and, then, testis fragments were collected, fixed in Karnovsky and post-fixed in osmiu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Fernandes Boaro Martins,Márcia Regina, Carvalho Pinto e Silva,José Ricardo de
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2005
Materias:	Spermiogenesis Spermatids Mice
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000400007
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022005000400007
record_format	dspace
spelling	oai:scielo:S0717-950220050004000072006-01-19Ultrastructural Aspects of Spermatids in Isogenic Black Mouse C57BL6JFernandes Boaro Martins,Márcia ReginaCarvalho Pinto e Silva,José Ricardo de Spermiogenesis Spermatids Mice The objective of this study was to describe some changes in spermatids differentiating events during spermiogenesis, leading to spermatozoa final formation. The animals were sacrificed with ethylic acid inhalation and, then, testis fragments were collected, fixed in Karnovsky and post-fixed in osmium tetroxide. After dehydration, the sections were stained with uranyl acetate 2%. It was observed in early spermatids at Golgi phase, formation of acrosomal granule contained in the acrosomal vesicle, spheroidal nucleus with rugous reticulum around it, and Golgi apparatus made up of lamellae and vesicular structures. At cephalic hood phase, the granule suffers flattening and the marginal fossa is formed. In both phases, it was possible to observe the presence of multivesicular body. At acrosomal phase, the acrosomal system is set up, and several enzymes of endoplasmatic reticulum as well chromatoid body are observed in the cytoplasm. At the last phase, that is, maturation, the nucleus becomes more elongated, the endoplasmatic reticulum regresses, the Golgi apparatus approaches to the flagellum and mitochondrias align next to itinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.23 n.4 20052005-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000400007en10.4067/S0717-95022005000400007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Spermiogenesis Spermatids Mice
spellingShingle	Spermiogenesis Spermatids Mice Fernandes Boaro Martins,Márcia Regina Carvalho Pinto e Silva,José Ricardo de Ultrastructural Aspects of Spermatids in Isogenic Black Mouse C57BL6J
description	The objective of this study was to describe some changes in spermatids differentiating events during spermiogenesis, leading to spermatozoa final formation. The animals were sacrificed with ethylic acid inhalation and, then, testis fragments were collected, fixed in Karnovsky and post-fixed in osmium tetroxide. After dehydration, the sections were stained with uranyl acetate 2%. It was observed in early spermatids at Golgi phase, formation of acrosomal granule contained in the acrosomal vesicle, spheroidal nucleus with rugous reticulum around it, and Golgi apparatus made up of lamellae and vesicular structures. At cephalic hood phase, the granule suffers flattening and the marginal fossa is formed. In both phases, it was possible to observe the presence of multivesicular body. At acrosomal phase, the acrosomal system is set up, and several enzymes of endoplasmatic reticulum as well chromatoid body are observed in the cytoplasm. At the last phase, that is, maturation, the nucleus becomes more elongated, the endoplasmatic reticulum regresses, the Golgi apparatus approaches to the flagellum and mitochondrias align next to it
author	Fernandes Boaro Martins,Márcia Regina Carvalho Pinto e Silva,José Ricardo de
author_facet	Fernandes Boaro Martins,Márcia Regina Carvalho Pinto e Silva,José Ricardo de
author_sort	Fernandes Boaro Martins,Márcia Regina
title	Ultrastructural Aspects of Spermatids in Isogenic Black Mouse C57BL6J
title_short	Ultrastructural Aspects of Spermatids in Isogenic Black Mouse C57BL6J
title_full	Ultrastructural Aspects of Spermatids in Isogenic Black Mouse C57BL6J
title_fullStr	Ultrastructural Aspects of Spermatids in Isogenic Black Mouse C57BL6J
title_full_unstemmed	Ultrastructural Aspects of Spermatids in Isogenic Black Mouse C57BL6J
title_sort	ultrastructural aspects of spermatids in isogenic black mouse c57bl6j
publisher	Sociedad Chilena de Anatomía
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000400007
work_keys_str_mv	AT fernandesboaromartinsmarciaregina ultrastructuralaspectsofspermatidsinisogenicblackmousec57bl6j AT carvalhopintoesilvajosericardode ultrastructuralaspectsofspermatidsinisogenicblackmousec57bl6j
_version_	1718444570589528064

Ultrastructural Aspects of Spermatids in Isogenic Black Mouse C57BL6J

Ejemplares similares