Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion

The otic ganglion is a cranial component of the parasympathetic division of the autonomic nervous system (ANS). Similar to other parasympathetic ganglia, otic ganglion presents multipolar neurons that are completely surrounded by satellite cells and intercellular substance as well, which allow us to...

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Autores principales:	Chagas,M. A., Babinski,M. A., Abidu-Figueiredo,M., Costa,W. S.
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2006
Materias:	Rat Otic ganglion Autonomic nervous system Histochemistry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000100015
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spelling	oai:scielo:S0717-950220060001000152006-09-07Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic GanglionChagas,M. A.Babinski,M. A.Abidu-Figueiredo,M.Costa,W. S. Rat Otic ganglion Autonomic nervous system Histochemistry The otic ganglion is a cranial component of the parasympathetic division of the autonomic nervous system (ANS). Similar to other parasympathetic ganglia, otic ganglion presents multipolar neurons that are completely surrounded by satellite cells and intercellular substance as well, which allow us to use this ganglion as a good experimental model for studying the relationship neuron / extracellular matrix. We have studied rat otic ganglion in 10 animals through light microscopy. After routine histological methods, 5 µm sections were obtained and coloured by Gomori`s trichrome, periodic acid-Schiff (PAS), Alcian Blue pH 2.5 and pH1.0, acetylation + PAS, acetylation + deacetylation + PAS, acid hydrolysis + PAS, phenylhydrazine + PAS and thiosemicarbazide + PAS. The presence of neutral glycoproteins was demonstrated by PAS reactivity. PAS inhibition following Alcian Blue staining in pH 2.5 and 1.0 showed the presence of a small quantity of acid glycoprotein. The extracellular matrix analysis showed the presence of neutral and acid glycoconjugates. These findings suggests a mutual interaction and a complex role in ganglionic physiologyinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.24 n.1 20062006-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000100015en10.4067/S0717-95022006000100015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Rat Otic ganglion Autonomic nervous system Histochemistry
spellingShingle	Rat Otic ganglion Autonomic nervous system Histochemistry Chagas,M. A. Babinski,M. A. Abidu-Figueiredo,M. Costa,W. S. Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion
description	The otic ganglion is a cranial component of the parasympathetic division of the autonomic nervous system (ANS). Similar to other parasympathetic ganglia, otic ganglion presents multipolar neurons that are completely surrounded by satellite cells and intercellular substance as well, which allow us to use this ganglion as a good experimental model for studying the relationship neuron / extracellular matrix. We have studied rat otic ganglion in 10 animals through light microscopy. After routine histological methods, 5 µm sections were obtained and coloured by Gomori`s trichrome, periodic acid-Schiff (PAS), Alcian Blue pH 2.5 and pH1.0, acetylation + PAS, acetylation + deacetylation + PAS, acid hydrolysis + PAS, phenylhydrazine + PAS and thiosemicarbazide + PAS. The presence of neutral glycoproteins was demonstrated by PAS reactivity. PAS inhibition following Alcian Blue staining in pH 2.5 and 1.0 showed the presence of a small quantity of acid glycoprotein. The extracellular matrix analysis showed the presence of neutral and acid glycoconjugates. These findings suggests a mutual interaction and a complex role in ganglionic physiology
author	Chagas,M. A. Babinski,M. A. Abidu-Figueiredo,M. Costa,W. S.
author_facet	Chagas,M. A. Babinski,M. A. Abidu-Figueiredo,M. Costa,W. S.
author_sort	Chagas,M. A.
title	Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion
title_short	Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion
title_full	Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion
title_fullStr	Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion
title_full_unstemmed	Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion
title_sort	histochemical features of the extracellular matrix in rattus rattus norvegicus otic ganglion
publisher	Sociedad Chilena de Anatomía
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000100015
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Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion

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