Action of Trivalent Chromium on Rat Liver Structure. Histometric and Haematological Studies

The trace mineral trivalent chromium (Cr3+) is an essential nutrient involved in the regulation of carbohydrate, lipid and protein metabolism via an enhancement of insulin action. The present work had as objective to characterize histopathologically and histometrically the hepatic alterations of fem...

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Autores principales:	Silva,Rogério Ferreira da, Lopes,Ruberval A, Sala,Miguel A, Vinha,Dionísio, Regalo,Simone C. H., Souza,Ana Maria de, Gregório,Zita Maria de O
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2006
Materias:	Liver Rat Chromium Morphometry Hematology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300014
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spelling	oai:scielo:S0717-950220060003000142006-08-28Action of Trivalent Chromium on Rat Liver Structure. Histometric and Haematological StudiesSilva,Rogério Ferreira daLopes,Ruberval ASala,Miguel AVinha,DionísioRegalo,Simone C. H.Souza,Ana Maria deGregório,Zita Maria de O Liver Rat Chromium Morphometry Hematology The trace mineral trivalent chromium (Cr3+) is an essential nutrient involved in the regulation of carbohydrate, lipid and protein metabolism via an enhancement of insulin action. The present work had as objective to characterize histopathologically and histometrically the hepatic alterations of female Wistar rats, determined by the chromium III administration in drinking water. Adult rats received ration and drinking water ad libitum containing 300 or 500 mg/l chromium III during 4 months. Control animals received only water and ration. All animals were sacrificed by lethal dose of anesthetic. Samples of liver were fixed in 10% formalin for 24 h. Tissues for microscopical pathology were processed using standard procedures. Paraffin sections prepared at 6 µm were stained with haematoxylin and eosin and PAS. Besides the histopathological exam, histometrical techniques were used. The blood was collected and processed for hematological study. Histopathological analysis revealed periportal, midzonal and pericentrilobular zones with parenchyma cells with varying degrees of vacuolation. Many hepatocytes are ballooned and the nuclei were in lysis. The centrilobular vein was dilated and congested. Dilated sinusoids containing erythrocytes were observed. The portal area showed fibrosis, biliar duct proliferation with small cells. Histometric study showed increased cytoplasm and cell volumes, and small values for number of hepatocytes per mm³. Lymphopenia was observed in 500 mg/l Cr3+/l treated animals. These results indicate that the chromium III has a direct participation in liver structures alterationsinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.24 n.2 20062006-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300014en10.4067/S0717-95022006000300014
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Liver Rat Chromium Morphometry Hematology
spellingShingle	Liver Rat Chromium Morphometry Hematology Silva,Rogério Ferreira da Lopes,Ruberval A Sala,Miguel A Vinha,Dionísio Regalo,Simone C. H. Souza,Ana Maria de Gregório,Zita Maria de O Action of Trivalent Chromium on Rat Liver Structure. Histometric and Haematological Studies
description	The trace mineral trivalent chromium (Cr3+) is an essential nutrient involved in the regulation of carbohydrate, lipid and protein metabolism via an enhancement of insulin action. The present work had as objective to characterize histopathologically and histometrically the hepatic alterations of female Wistar rats, determined by the chromium III administration in drinking water. Adult rats received ration and drinking water ad libitum containing 300 or 500 mg/l chromium III during 4 months. Control animals received only water and ration. All animals were sacrificed by lethal dose of anesthetic. Samples of liver were fixed in 10% formalin for 24 h. Tissues for microscopical pathology were processed using standard procedures. Paraffin sections prepared at 6 µm were stained with haematoxylin and eosin and PAS. Besides the histopathological exam, histometrical techniques were used. The blood was collected and processed for hematological study. Histopathological analysis revealed periportal, midzonal and pericentrilobular zones with parenchyma cells with varying degrees of vacuolation. Many hepatocytes are ballooned and the nuclei were in lysis. The centrilobular vein was dilated and congested. Dilated sinusoids containing erythrocytes were observed. The portal area showed fibrosis, biliar duct proliferation with small cells. Histometric study showed increased cytoplasm and cell volumes, and small values for number of hepatocytes per mm³. Lymphopenia was observed in 500 mg/l Cr3+/l treated animals. These results indicate that the chromium III has a direct participation in liver structures alterations
author	Silva,Rogério Ferreira da Lopes,Ruberval A Sala,Miguel A Vinha,Dionísio Regalo,Simone C. H. Souza,Ana Maria de Gregório,Zita Maria de O
author_facet	Silva,Rogério Ferreira da Lopes,Ruberval A Sala,Miguel A Vinha,Dionísio Regalo,Simone C. H. Souza,Ana Maria de Gregório,Zita Maria de O
author_sort	Silva,Rogério Ferreira da
title	Action of Trivalent Chromium on Rat Liver Structure. Histometric and Haematological Studies
title_short	Action of Trivalent Chromium on Rat Liver Structure. Histometric and Haematological Studies
title_full	Action of Trivalent Chromium on Rat Liver Structure. Histometric and Haematological Studies
title_fullStr	Action of Trivalent Chromium on Rat Liver Structure. Histometric and Haematological Studies
title_full_unstemmed	Action of Trivalent Chromium on Rat Liver Structure. Histometric and Haematological Studies
title_sort	action of trivalent chromium on rat liver structure. histometric and haematological studies
publisher	Sociedad Chilena de Anatomía
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300014
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Action of Trivalent Chromium on Rat Liver Structure. Histometric and Haematological Studies

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