Obtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer Technique

The objective of this project is to produce pudu embryos, obtained by means of fibroblast transfer taken from the ear of one animal, to the oocytes of a domestic ruminant such as cattle. In further work, the pudu embryos will be transferred to the uterus of synchronized recipient females of another...

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Autores principales:	Venegas,Felipe, Guillomot,Michel, Vignon,Xavier, Servely,Jean-Luc, Audouard,Chistophe, Montiel,Enrique, Le Bourhis,Daniel, Perón,Sandrine, Soto,Pilar, Rojas,Mariana
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2006
Materias:	Pudu deer Cervidae Ruminant Cloning
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300026
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spelling	oai:scielo:S0717-950220060003000262006-08-28Obtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer TechniqueVenegas,FelipeGuillomot,MichelVignon,XavierServely,Jean-LucAudouard,ChistopheMontiel,EnriqueLe Bourhis,DanielPerón,SandrineSoto,PilarRojas,Mariana Pudu deer Cervidae Ruminant Cloning The objective of this project is to produce pudu embryos, obtained by means of fibroblast transfer taken from the ear of one animal, to the oocytes of a domestic ruminant such as cattle. In further work, the pudu embryos will be transferred to the uterus of synchronized recipient females of another species. Biopsies 1mm in size were obtained from the outer rim of the ear of two male pudu deer belonging to the Buin Zoological park, Santiago, Chile. The cell lines have been established and kept according to protocols used in cattle. The oocytes are obtained through a puncture in the oocyte-cumulus complex (OCC) from cow ovaries recovered from the butcher. Each oocyte is enucleated and fused with an isolated fibroblast which is inserted beneath the pellucid zone. The fusion of cell membranes is achieved by means of electric shocks. In respect to the timetable, we have observed that a stage of two blastomers is reached on the second day, and morulae of 8 to 16 cells on the third day. On the fourth day it has differenciated as a blastocyst and on the seventh day it finally hacht from the pellucid zone. The obtainment of embryonic blastocytes indicates that it is possible to obtain pudu embryos through heterospecific cloning, even though the percentage of success is relatively low. The viability of the embryos obtained in this manner after in uterus transfer remains still to be verifiedinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.24 n.2 20062006-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300026en10.4067/S0717-95022006000300026
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Pudu deer Cervidae Ruminant Cloning
spellingShingle	Pudu deer Cervidae Ruminant Cloning Venegas,Felipe Guillomot,Michel Vignon,Xavier Servely,Jean-Luc Audouard,Chistophe Montiel,Enrique Le Bourhis,Daniel Perón,Sandrine Soto,Pilar Rojas,Mariana Obtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer Technique
description	The objective of this project is to produce pudu embryos, obtained by means of fibroblast transfer taken from the ear of one animal, to the oocytes of a domestic ruminant such as cattle. In further work, the pudu embryos will be transferred to the uterus of synchronized recipient females of another species. Biopsies 1mm in size were obtained from the outer rim of the ear of two male pudu deer belonging to the Buin Zoological park, Santiago, Chile. The cell lines have been established and kept according to protocols used in cattle. The oocytes are obtained through a puncture in the oocyte-cumulus complex (OCC) from cow ovaries recovered from the butcher. Each oocyte is enucleated and fused with an isolated fibroblast which is inserted beneath the pellucid zone. The fusion of cell membranes is achieved by means of electric shocks. In respect to the timetable, we have observed that a stage of two blastomers is reached on the second day, and morulae of 8 to 16 cells on the third day. On the fourth day it has differenciated as a blastocyst and on the seventh day it finally hacht from the pellucid zone. The obtainment of embryonic blastocytes indicates that it is possible to obtain pudu embryos through heterospecific cloning, even though the percentage of success is relatively low. The viability of the embryos obtained in this manner after in uterus transfer remains still to be verified
author	Venegas,Felipe Guillomot,Michel Vignon,Xavier Servely,Jean-Luc Audouard,Chistophe Montiel,Enrique Le Bourhis,Daniel Perón,Sandrine Soto,Pilar Rojas,Mariana
author_facet	Venegas,Felipe Guillomot,Michel Vignon,Xavier Servely,Jean-Luc Audouard,Chistophe Montiel,Enrique Le Bourhis,Daniel Perón,Sandrine Soto,Pilar Rojas,Mariana
author_sort	Venegas,Felipe
title	Obtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer Technique
title_short	Obtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer Technique
title_full	Obtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer Technique
title_fullStr	Obtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer Technique
title_full_unstemmed	Obtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer Technique
title_sort	obtaiment of pudu (pudu pudu) deer embryos by the somatic nuclear transfer technique
publisher	Sociedad Chilena de Anatomía
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300026
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Obtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer Technique

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