Histological and Histochemical Studies on the Effects of Methotrexate on the Liver of Adult Male Albino Rat

Methotrexate (MTX) is widely used in the therapy of various types of malignancy. The present work was designed to investigate the histological and histochemical changes in the liver of albino rat following methotrexate administration. Fifteen adult male albino rats were used in the present work. The...

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Autor principal:	Al-Motabagani,Mohamed Akram
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2006
Materias:	Methotrexate Hepatocytes Hepatic lobules lycogen Enzyme activity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400020
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spelling	oai:scielo:S0717-950220060004000202007-01-24Histological and Histochemical Studies on the Effects of Methotrexate on the Liver of Adult Male Albino RatAl-Motabagani,Mohamed Akram Methotrexate Hepatocytes Hepatic lobules lycogen Enzyme activity Methotrexate (MTX) is widely used in the therapy of various types of malignancy. The present work was designed to investigate the histological and histochemical changes in the liver of albino rat following methotrexate administration. Fifteen adult male albino rats were used in the present work. They were divided into three main groups: Group I was kept without treatment and served as control. Groups II and III were given intraperitoneal injections of normal saline and MTX, respectively, at a dosage of (0.5 mg/Kg) twice weekly for total durations of 3, 6 and 9 weeks. The rats were sacrificed and the livers were excised and processed for histological and histochemical study. Examination of sections of the livers of group III showed mononuclear cell infiltration and an increase in the amount of collagen fibers in the portal tracts. There were focal areas of liver cell necrosis with distortion of the normal hepatic architecture. Moreover, there was a gradual and progressive decrease of glycogen content in the hepatocytes. Furthermore, succinic dehydrogenase and alkaline phosphatase activity were also decreased. In addition there was an increase in acid phosphatase activities in the degenerated areas and loss of activities in areas of massive cellular necrosis. It was concluded that repeated injections of MTX causes hepatic damage of a definite magnitude. This hepatotoxicity progressed with increasing cumulative doses of methotrexate. The present study provided further evidence to the cytotoxic potency of this antifolateinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.24 n.3 20062006-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400020en10.4067/S0717-95022006000400020
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Methotrexate Hepatocytes Hepatic lobules lycogen Enzyme activity
spellingShingle	Methotrexate Hepatocytes Hepatic lobules lycogen Enzyme activity Al-Motabagani,Mohamed Akram Histological and Histochemical Studies on the Effects of Methotrexate on the Liver of Adult Male Albino Rat
description	Methotrexate (MTX) is widely used in the therapy of various types of malignancy. The present work was designed to investigate the histological and histochemical changes in the liver of albino rat following methotrexate administration. Fifteen adult male albino rats were used in the present work. They were divided into three main groups: Group I was kept without treatment and served as control. Groups II and III were given intraperitoneal injections of normal saline and MTX, respectively, at a dosage of (0.5 mg/Kg) twice weekly for total durations of 3, 6 and 9 weeks. The rats were sacrificed and the livers were excised and processed for histological and histochemical study. Examination of sections of the livers of group III showed mononuclear cell infiltration and an increase in the amount of collagen fibers in the portal tracts. There were focal areas of liver cell necrosis with distortion of the normal hepatic architecture. Moreover, there was a gradual and progressive decrease of glycogen content in the hepatocytes. Furthermore, succinic dehydrogenase and alkaline phosphatase activity were also decreased. In addition there was an increase in acid phosphatase activities in the degenerated areas and loss of activities in areas of massive cellular necrosis. It was concluded that repeated injections of MTX causes hepatic damage of a definite magnitude. This hepatotoxicity progressed with increasing cumulative doses of methotrexate. The present study provided further evidence to the cytotoxic potency of this antifolate
author	Al-Motabagani,Mohamed Akram
author_facet	Al-Motabagani,Mohamed Akram
author_sort	Al-Motabagani,Mohamed Akram
title	Histological and Histochemical Studies on the Effects of Methotrexate on the Liver of Adult Male Albino Rat
title_short	Histological and Histochemical Studies on the Effects of Methotrexate on the Liver of Adult Male Albino Rat
title_full	Histological and Histochemical Studies on the Effects of Methotrexate on the Liver of Adult Male Albino Rat
title_fullStr	Histological and Histochemical Studies on the Effects of Methotrexate on the Liver of Adult Male Albino Rat
title_full_unstemmed	Histological and Histochemical Studies on the Effects of Methotrexate on the Liver of Adult Male Albino Rat
title_sort	histological and histochemical studies on the effects of methotrexate on the liver of adult male albino rat
publisher	Sociedad Chilena de Anatomía
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400020
work_keys_str_mv	AT almotabaganimohamedakram histologicalandhistochemicalstudiesontheeffectsofmethotrexateontheliverofadultmalealbinorat
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Histological and Histochemical Studies on the Effects of Methotrexate on the Liver of Adult Male Albino Rat

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