Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet

This study evaluated whether a high fat diet (HFC group) induces overweight, hepatic steatosis and plasma lipoproteins level alteration compared to standard chow diet (SC group). Female mice were submitted to each diet over 6 months. Body mass and food intake were evaluated weekly throughout the exp...

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Autores principales:	Neves,Renata Heisler, Barros Alencar,Alba Cristina Miranda de, Aguila,Marcia Barbosa, Mandarim-de-Lacerda,Carlos Alberto, Machado-Silva,José Roberto, Gomes,Delir Corrêa
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2006
Materias:	Hypercholesterolemia Experimental model Mice Cholesterol Diet Liver
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500018
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spelling	oai:scielo:S0717-950220060005000182007-05-09Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat DietNeves,Renata HeislerBarros Alencar,Alba Cristina Miranda deAguila,Marcia BarbosaMandarim-de-Lacerda,Carlos AlbertoMachado-Silva,José RobertoGomes,Delir Corrêa Hypercholesterolemia Experimental model Mice Cholesterol Diet Liver This study evaluated whether a high fat diet (HFC group) induces overweight, hepatic steatosis and plasma lipoproteins level alteration compared to standard chow diet (SC group). Female mice were submitted to each diet over 6 months. Body mass and food intake were evaluated weekly throughout the experiment. Total cholesterol, TG, LDL-c, HDL-c and VLDL-c were analyzed. Mice were sacrificed to remove liver, spleen, heart and intestine. The volume of the organs was determined according to the submersion method. Fixed livers were embedded in paraffin and stained with hematoxylin and eosin and Masson's trichrome. The analysis used a video microscope system and a test-system with 42 test-points. The volume density was estimated for hepatocytes, steatosis and sinusoids. Animals fed HFC had smaller chow intake than SC group. HFC group presented body mass greater than SC. Animals fed HFC showed heavier liver and spleen and lighter intestine than SC (p<0.05), heart mass was not significant between groups. Plasma lipoproteins differed between groups (p<0.05) except VLDL-c and TG fractions. The liver structure was without major alteration in SC group however, HFC mice group showed different degrees of fatty degeneration with micro- and macrovesicular steatosis dispersed in all liver with typical peri-cellular/peri-sinusoidal fibrosis. The quantitative study showed significant (p<0.05) volume density reduction for hepatocytes and sinusoids. In conclusion, our results clearly show that hepatic steatosis can be induced in mouse by such a fat-rich diet without any toxin ingestion, alimentary deficiency and genes depletioninfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.24 n.4 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500018en10.4067/S0717-95022006000500018
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Hypercholesterolemia Experimental model Mice Cholesterol Diet Liver
spellingShingle	Hypercholesterolemia Experimental model Mice Cholesterol Diet Liver Neves,Renata Heisler Barros Alencar,Alba Cristina Miranda de Aguila,Marcia Barbosa Mandarim-de-Lacerda,Carlos Alberto Machado-Silva,José Roberto Gomes,Delir Corrêa Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
description	This study evaluated whether a high fat diet (HFC group) induces overweight, hepatic steatosis and plasma lipoproteins level alteration compared to standard chow diet (SC group). Female mice were submitted to each diet over 6 months. Body mass and food intake were evaluated weekly throughout the experiment. Total cholesterol, TG, LDL-c, HDL-c and VLDL-c were analyzed. Mice were sacrificed to remove liver, spleen, heart and intestine. The volume of the organs was determined according to the submersion method. Fixed livers were embedded in paraffin and stained with hematoxylin and eosin and Masson's trichrome. The analysis used a video microscope system and a test-system with 42 test-points. The volume density was estimated for hepatocytes, steatosis and sinusoids. Animals fed HFC had smaller chow intake than SC group. HFC group presented body mass greater than SC. Animals fed HFC showed heavier liver and spleen and lighter intestine than SC (p<0.05), heart mass was not significant between groups. Plasma lipoproteins differed between groups (p<0.05) except VLDL-c and TG fractions. The liver structure was without major alteration in SC group however, HFC mice group showed different degrees of fatty degeneration with micro- and macrovesicular steatosis dispersed in all liver with typical peri-cellular/peri-sinusoidal fibrosis. The quantitative study showed significant (p<0.05) volume density reduction for hepatocytes and sinusoids. In conclusion, our results clearly show that hepatic steatosis can be induced in mouse by such a fat-rich diet without any toxin ingestion, alimentary deficiency and genes depletion
author	Neves,Renata Heisler Barros Alencar,Alba Cristina Miranda de Aguila,Marcia Barbosa Mandarim-de-Lacerda,Carlos Alberto Machado-Silva,José Roberto Gomes,Delir Corrêa
author_facet	Neves,Renata Heisler Barros Alencar,Alba Cristina Miranda de Aguila,Marcia Barbosa Mandarim-de-Lacerda,Carlos Alberto Machado-Silva,José Roberto Gomes,Delir Corrêa
author_sort	Neves,Renata Heisler
title	Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
title_short	Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
title_full	Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
title_fullStr	Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
title_full_unstemmed	Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
title_sort	somatic, biochemical and hepatic alterations in wild type mice chronically fed high fat diet
publisher	Sociedad Chilena de Anatomía
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500018
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Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet

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