Histological Changes of the Ovary of Adult Mice Exposed Prenatally to Flunitrazepam

Flunitrazepam (FNZ) is a minor tranquillizer involving allosteric modulation of the GABA receptor complex. It is a ligand of the peripheral benzodiazepine receptor (PBR) that participates in cholesterol transport in steroidogenic organs. The purpose was to investigate whether a single oral dose of F...

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Autores principales:	Aguirre -Samudio,Ana Julia, de la Fuente-Juárez,Graciela, Márquez-Orozco,Amalia, Márquez-Orozco,María Cristina
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2006
Materias:	Benzodiazepine Flunitrazepam Atretic follicles Corpus luteum Prenatal exposure
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500022
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spelling	oai:scielo:S0717-950220060005000222007-05-09Histological Changes of the Ovary of Adult Mice Exposed Prenatally to FlunitrazepamAguirre -Samudio,Ana Juliade la Fuente-Juárez,GracielaMárquez-Orozco,AmaliaMárquez-Orozco,María Cristina Benzodiazepine Flunitrazepam Atretic follicles Corpus luteum Prenatal exposure Flunitrazepam (FNZ) is a minor tranquillizer involving allosteric modulation of the GABA receptor complex. It is a ligand of the peripheral benzodiazepine receptor (PBR) that participates in cholesterol transport in steroidogenic organs. The purpose was to investigate whether a single oral dose of FNZ of 2.5 mg/kg of body weight (bw), administered on day 6 of gestation, alters the structure of the adult ovary of mouse offsprings at 2 months of age. The mouse offsprings of the in utero FNZ-treated group and those of the control group (C) were killed. Ovaries were obtained in early estrous, fixed in Zenker solution, and processed by routine histological techniques. Serial sections were observed under light microscopy to determine the characteristics and quantity of follicles in different stages of development and of the corpus luteum. The ovarian tissues from the FNZ group depicted a great staining affinity, enlarged nuclei with abundant heterochromatin clumps. The quantity of primordial, primary and secondary normal follicles in the FNZ group decreased significantly (p< 0.01). The number of primary atretic follicles increased (p<0.01) and the secondary ones remained constant as in group C. Histological changes and statistical data suggest that FNZ produces long-lasting epigenetic or genotoxic effects in follicular and corpus luteum cells of the ovary of prenatally FNZ-treated miceinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.24 n.4 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500022en10.4067/S0717-95022006000500022
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Benzodiazepine Flunitrazepam Atretic follicles Corpus luteum Prenatal exposure
spellingShingle	Benzodiazepine Flunitrazepam Atretic follicles Corpus luteum Prenatal exposure Aguirre -Samudio,Ana Julia de la Fuente-Juárez,Graciela Márquez-Orozco,Amalia Márquez-Orozco,María Cristina Histological Changes of the Ovary of Adult Mice Exposed Prenatally to Flunitrazepam
description	Flunitrazepam (FNZ) is a minor tranquillizer involving allosteric modulation of the GABA receptor complex. It is a ligand of the peripheral benzodiazepine receptor (PBR) that participates in cholesterol transport in steroidogenic organs. The purpose was to investigate whether a single oral dose of FNZ of 2.5 mg/kg of body weight (bw), administered on day 6 of gestation, alters the structure of the adult ovary of mouse offsprings at 2 months of age. The mouse offsprings of the in utero FNZ-treated group and those of the control group (C) were killed. Ovaries were obtained in early estrous, fixed in Zenker solution, and processed by routine histological techniques. Serial sections were observed under light microscopy to determine the characteristics and quantity of follicles in different stages of development and of the corpus luteum. The ovarian tissues from the FNZ group depicted a great staining affinity, enlarged nuclei with abundant heterochromatin clumps. The quantity of primordial, primary and secondary normal follicles in the FNZ group decreased significantly (p< 0.01). The number of primary atretic follicles increased (p<0.01) and the secondary ones remained constant as in group C. Histological changes and statistical data suggest that FNZ produces long-lasting epigenetic or genotoxic effects in follicular and corpus luteum cells of the ovary of prenatally FNZ-treated mice
author	Aguirre -Samudio,Ana Julia de la Fuente-Juárez,Graciela Márquez-Orozco,Amalia Márquez-Orozco,María Cristina
author_facet	Aguirre -Samudio,Ana Julia de la Fuente-Juárez,Graciela Márquez-Orozco,Amalia Márquez-Orozco,María Cristina
author_sort	Aguirre -Samudio,Ana Julia
title	Histological Changes of the Ovary of Adult Mice Exposed Prenatally to Flunitrazepam
title_short	Histological Changes of the Ovary of Adult Mice Exposed Prenatally to Flunitrazepam
title_full	Histological Changes of the Ovary of Adult Mice Exposed Prenatally to Flunitrazepam
title_fullStr	Histological Changes of the Ovary of Adult Mice Exposed Prenatally to Flunitrazepam
title_full_unstemmed	Histological Changes of the Ovary of Adult Mice Exposed Prenatally to Flunitrazepam
title_sort	histological changes of the ovary of adult mice exposed prenatally to flunitrazepam
publisher	Sociedad Chilena de Anatomía
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500022
work_keys_str_mv	AT aguirresamudioanajulia histologicalchangesoftheovaryofadultmiceexposedprenatallytoflunitrazepam AT delafuentejuarezgraciela histologicalchangesoftheovaryofadultmiceexposedprenatallytoflunitrazepam AT marquezorozcoamalia histologicalchangesoftheovaryofadultmiceexposedprenatallytoflunitrazepam AT marquezorozcomariacristina histologicalchangesoftheovaryofadultmiceexposedprenatallytoflunitrazepam
_version_	1718444592602284032

Histological Changes of the Ovary of Adult Mice Exposed Prenatally to Flunitrazepam

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