Effect of Thyroxine and Propylthiouracil in Ehrlich Ascitic Tumor Cells

The main purpose of this study was to investigate the effect of thyroxine and PTU in ascitic Ehrlich tumor cells. Tumor was implanted in 30 female mice distributed in three groups: treated with PTU, treated with thyroxine and control. Each group received an intraperitoneal injection of neoplastic ce...

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Autores principales:	Freitas,E. S., Leite,E. D., Silva,A. E., Ocarino,N. M., Ferreira,E., Gomes,M. G., Cassali,G. D., Serakides,R
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2006
Materias:	Cytology Ehrlich tumor Mouse NORs Propylthiouracil thyroxine
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500024
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spelling	oai:scielo:S0717-950220060005000242007-05-09Effect of Thyroxine and Propylthiouracil in Ehrlich Ascitic Tumor CellsFreitas,E. S.Leite,E. D.Silva,A. E.Ocarino,N. M.Ferreira,E.Gomes,M. G.Cassali,G. D.Serakides,R Cytology Ehrlich tumor Mouse NORs Propylthiouracil thyroxine The main purpose of this study was to investigate the effect of thyroxine and PTU in ascitic Ehrlich tumor cells. Tumor was implanted in 30 female mice distributed in three groups: treated with PTU, treated with thyroxine and control. Each group received an intraperitoneal injection of neoplastic cells, pre-incubated with sterile solutions of PTU, thyroxine and distilled water, respectively. On the fifth and seventh days after inoculation, animals received an intraperitoneal injection of the respective solutions. On the tenth day after inoculation, animals were sacrificed. Volume of ascitic liquid, number of neoplastic cells/ml and percentage of viable cells were determined. Ascitic liquid smears were carried out for tumor cytological evaluation. There was no difference among groups regarding ascitic liquid and as for the number and viability of tumor cells. However, cells under the effect of thyroxine presented significantly larger mean of nuclear diameter, size and number of nucleolus organizer regions. In this group, there was a predominance of clear, round cells with abundant eosinophilic and very vacuolated cytoplasm with little defined edges. Under the PTU effect, tumor cells were small with hyperchromatic nucleus and the same number of NORs as the control group. It was concluded that PTU and thyroxine have not changed the number and viability of cells after 10 days of tumor inoculation but they changed significantly cell characteristics. Whilst thyroxine increases cell size and the number of NORs of ascitic Ehrlich tumor cells, PTU causes an opposite effectinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.24 n.4 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500024en10.4067/S0717-95022006000500024
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cytology Ehrlich tumor Mouse NORs Propylthiouracil thyroxine
spellingShingle	Cytology Ehrlich tumor Mouse NORs Propylthiouracil thyroxine Freitas,E. S. Leite,E. D. Silva,A. E. Ocarino,N. M. Ferreira,E. Gomes,M. G. Cassali,G. D. Serakides,R Effect of Thyroxine and Propylthiouracil in Ehrlich Ascitic Tumor Cells
description	The main purpose of this study was to investigate the effect of thyroxine and PTU in ascitic Ehrlich tumor cells. Tumor was implanted in 30 female mice distributed in three groups: treated with PTU, treated with thyroxine and control. Each group received an intraperitoneal injection of neoplastic cells, pre-incubated with sterile solutions of PTU, thyroxine and distilled water, respectively. On the fifth and seventh days after inoculation, animals received an intraperitoneal injection of the respective solutions. On the tenth day after inoculation, animals were sacrificed. Volume of ascitic liquid, number of neoplastic cells/ml and percentage of viable cells were determined. Ascitic liquid smears were carried out for tumor cytological evaluation. There was no difference among groups regarding ascitic liquid and as for the number and viability of tumor cells. However, cells under the effect of thyroxine presented significantly larger mean of nuclear diameter, size and number of nucleolus organizer regions. In this group, there was a predominance of clear, round cells with abundant eosinophilic and very vacuolated cytoplasm with little defined edges. Under the PTU effect, tumor cells were small with hyperchromatic nucleus and the same number of NORs as the control group. It was concluded that PTU and thyroxine have not changed the number and viability of cells after 10 days of tumor inoculation but they changed significantly cell characteristics. Whilst thyroxine increases cell size and the number of NORs of ascitic Ehrlich tumor cells, PTU causes an opposite effect
author	Freitas,E. S. Leite,E. D. Silva,A. E. Ocarino,N. M. Ferreira,E. Gomes,M. G. Cassali,G. D. Serakides,R
author_facet	Freitas,E. S. Leite,E. D. Silva,A. E. Ocarino,N. M. Ferreira,E. Gomes,M. G. Cassali,G. D. Serakides,R
author_sort	Freitas,E. S.
title	Effect of Thyroxine and Propylthiouracil in Ehrlich Ascitic Tumor Cells
title_short	Effect of Thyroxine and Propylthiouracil in Ehrlich Ascitic Tumor Cells
title_full	Effect of Thyroxine and Propylthiouracil in Ehrlich Ascitic Tumor Cells
title_fullStr	Effect of Thyroxine and Propylthiouracil in Ehrlich Ascitic Tumor Cells
title_full_unstemmed	Effect of Thyroxine and Propylthiouracil in Ehrlich Ascitic Tumor Cells
title_sort	effect of thyroxine and propylthiouracil in ehrlich ascitic tumor cells
publisher	Sociedad Chilena de Anatomía
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500024
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Effect of Thyroxine and Propylthiouracil in Ehrlich Ascitic Tumor Cells

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