Morphological and Histochemical Analysis of the Human Vestibular Fold

A morphological and histochemical study of the human vestibular fold was carried out using routine histological techniques. Seven µm-thick histo logical sections stained with hematoxylin-eosin (HE) and Callej a showed the presence of elastic collagen fibers and seromucous glands in the vestibular fo...

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Autores principales: Guida,Heraldo Lorena, Zorzetto,Neivo Luiz
Lenguaje:English
Publicado: Sociedad Chilena de Anatomía 2007
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300010
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spelling oai:scielo:S0717-950220070003000102007-10-30Morphological and Histochemical Analysis of the Human Vestibular FoldGuida,Heraldo LorenaZorzetto,Neivo Luiz Larynx Vestibular fold Morphology A morphological and histochemical study of the human vestibular fold was carried out using routine histological techniques. Seven µm-thick histo logical sections stained with hematoxylin-eosin (HE) and Callej a showed the presence of elastic collagen fibers and seromucous glands in the vestibular fold. Muscle fibers forming the ventricular muscle were also identified. Ultrastructural analyses of the epithelial layer by scanning electron microscopy (SEM) revealed ciliated cells and gland ducts opening on the epithelial surface. Histochemical analyses were performed on ventricular muscles submitted to nicotinamide-adenine-dinucleotide tetrazolium reductase (NADH-TR), succinate dehydrogenase (SDH), and myofibrillar adenosine triphosphatase (mATPase) reactions. Based on these reactions, it was observed that the muscle is formed by three types of muscle fibers: slow-twitch oxidative (SO), fast-twitch oxydative glycolytic (FOG) and fast-twitch glycolytic (FG) fibers distributed in a mosaic pattern. The fiber frequency was 22.7%, 69.9% and 7.4%, respectively. The higher frequency of SO and FOG fibers characterized the muscle as having aerobic metabolism and resistance to fatigue. The ventricular muscle was considered fast. The study of the neuromuscular junctions performed after nonspecific esterase reaction showed that they are of the en-plaque type and have multiple occurrences in the ventricular muscleinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.25 n.3 20072007-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300010en10.4067/S0717-95022007000300010
institution Scielo Chile
collection Scielo Chile
language English
topic Larynx
Vestibular fold
Morphology
spellingShingle Larynx
Vestibular fold
Morphology
Guida,Heraldo Lorena
Zorzetto,Neivo Luiz
Morphological and Histochemical Analysis of the Human Vestibular Fold
description A morphological and histochemical study of the human vestibular fold was carried out using routine histological techniques. Seven µm-thick histo logical sections stained with hematoxylin-eosin (HE) and Callej a showed the presence of elastic collagen fibers and seromucous glands in the vestibular fold. Muscle fibers forming the ventricular muscle were also identified. Ultrastructural analyses of the epithelial layer by scanning electron microscopy (SEM) revealed ciliated cells and gland ducts opening on the epithelial surface. Histochemical analyses were performed on ventricular muscles submitted to nicotinamide-adenine-dinucleotide tetrazolium reductase (NADH-TR), succinate dehydrogenase (SDH), and myofibrillar adenosine triphosphatase (mATPase) reactions. Based on these reactions, it was observed that the muscle is formed by three types of muscle fibers: slow-twitch oxidative (SO), fast-twitch oxydative glycolytic (FOG) and fast-twitch glycolytic (FG) fibers distributed in a mosaic pattern. The fiber frequency was 22.7%, 69.9% and 7.4%, respectively. The higher frequency of SO and FOG fibers characterized the muscle as having aerobic metabolism and resistance to fatigue. The ventricular muscle was considered fast. The study of the neuromuscular junctions performed after nonspecific esterase reaction showed that they are of the en-plaque type and have multiple occurrences in the ventricular muscle
author Guida,Heraldo Lorena
Zorzetto,Neivo Luiz
author_facet Guida,Heraldo Lorena
Zorzetto,Neivo Luiz
author_sort Guida,Heraldo Lorena
title Morphological and Histochemical Analysis of the Human Vestibular Fold
title_short Morphological and Histochemical Analysis of the Human Vestibular Fold
title_full Morphological and Histochemical Analysis of the Human Vestibular Fold
title_fullStr Morphological and Histochemical Analysis of the Human Vestibular Fold
title_full_unstemmed Morphological and Histochemical Analysis of the Human Vestibular Fold
title_sort morphological and histochemical analysis of the human vestibular fold
publisher Sociedad Chilena de Anatomía
publishDate 2007
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300010
work_keys_str_mv AT guidaheraldolorena morphologicalandhistochemicalanalysisofthehumanvestibularfold
AT zorzettoneivoluiz morphologicalandhistochemicalanalysisofthehumanvestibularfold
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