Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male Rat

The vomeronasal organ (VNO) is a tubular chemoreceptory organ which detects environmental pheromones and is essential for mammalian normal reproductive activity. A sensitive chemoreceptory neuroepithelium lines the concave VNO wall and the opposite, convex wall is lined by a seudostratified, recepto...

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Autores principales:	Núñez Chichet,M E, Genovese,P, Bielli,A
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2007
Materias:	Vomeronasal organ Chemoreception Development Histochemistry Immunohistochemistry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300018
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spelling	oai:scielo:S0717-950220070003000182007-10-30Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male RatNúñez Chichet,M EGenovese,PBielli,A Vomeronasal organ Chemoreception Development Histochemistry Immunohistochemistry The vomeronasal organ (VNO) is a tubular chemoreceptory organ which detects environmental pheromones and is essential for mammalian normal reproductive activity. A sensitive chemoreceptory neuroepithelium lines the concave VNO wall and the opposite, convex wall is lined by a seudostratified, receptor-free epithelium. The secretion of the Jacobson glands (JG) -tubuloacinary glands lying in the lamina propia of the VNO - is essential for pheromones contacting vomeronasal organ chemoreceptors. Tubuloacinary glands lying in the connective tissue of the nasal septum mucosa (Bowman glands), can be classified as ventral and dorsal, according to their location. Positivity to PAS and Alcyan blue reactions and androgen receptor immunolocalization was evaluated with a semiquantitative system (0,1, 2 or 3 crosses) in JG, ventral Bowman glands (VBG), dorsal Bowman glands (DBG), sensitive chemoreceptory neuroepithelium (Ne), receptor-free epithelium (RFE) and nasal septum respiratory epithelium (RE) histological slides of male rats at 5, 15, 25 and 35 days of age. The VBG and JG were intensely positive to PAS reaction since 5 days of age, and weakly positive to Alcyan blue reaction (AB) since 15 days of age, but not at 5 days of age. Both JG and VBG were strongly positive to androgen receptor immunolocalization at all ages studied (15, 25 and 35 days of age). DBG were always negative to both PAS and AB reactions. The NE and the RFE and nasal mucosa luminar epithelium were negative. To our knowledge, this is the first report of androgen receptor (AR) immunolocalization in the vomeronasal organ and the nasal septum mucosa. The present results also suggest an influence of androgens on the mechanism of pheromones contacting with the Ne of the VNO through regulation of the Jacobson glands secretioninfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.25 n.3 20072007-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300018en10.4067/S0717-95022007000300018
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Vomeronasal organ Chemoreception Development Histochemistry Immunohistochemistry
spellingShingle	Vomeronasal organ Chemoreception Development Histochemistry Immunohistochemistry Núñez Chichet,M E Genovese,P Bielli,A Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male Rat
description	The vomeronasal organ (VNO) is a tubular chemoreceptory organ which detects environmental pheromones and is essential for mammalian normal reproductive activity. A sensitive chemoreceptory neuroepithelium lines the concave VNO wall and the opposite, convex wall is lined by a seudostratified, receptor-free epithelium. The secretion of the Jacobson glands (JG) -tubuloacinary glands lying in the lamina propia of the VNO - is essential for pheromones contacting vomeronasal organ chemoreceptors. Tubuloacinary glands lying in the connective tissue of the nasal septum mucosa (Bowman glands), can be classified as ventral and dorsal, according to their location. Positivity to PAS and Alcyan blue reactions and androgen receptor immunolocalization was evaluated with a semiquantitative system (0,1, 2 or 3 crosses) in JG, ventral Bowman glands (VBG), dorsal Bowman glands (DBG), sensitive chemoreceptory neuroepithelium (Ne), receptor-free epithelium (RFE) and nasal septum respiratory epithelium (RE) histological slides of male rats at 5, 15, 25 and 35 days of age. The VBG and JG were intensely positive to PAS reaction since 5 days of age, and weakly positive to Alcyan blue reaction (AB) since 15 days of age, but not at 5 days of age. Both JG and VBG were strongly positive to androgen receptor immunolocalization at all ages studied (15, 25 and 35 days of age). DBG were always negative to both PAS and AB reactions. The NE and the RFE and nasal mucosa luminar epithelium were negative. To our knowledge, this is the first report of androgen receptor (AR) immunolocalization in the vomeronasal organ and the nasal septum mucosa. The present results also suggest an influence of androgens on the mechanism of pheromones contacting with the Ne of the VNO through regulation of the Jacobson glands secretion
author	Núñez Chichet,M E Genovese,P Bielli,A
author_facet	Núñez Chichet,M E Genovese,P Bielli,A
author_sort	Núñez Chichet,M E
title	Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male Rat
title_short	Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male Rat
title_full	Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male Rat
title_fullStr	Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male Rat
title_full_unstemmed	Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male Rat
title_sort	androgen receptor distribution, pas and alcyan blue reaction in the vomeronasal organ and the nasal septum mucosa of the developing male rat
publisher	Sociedad Chilena de Anatomía
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300018
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Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male Rat

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