Excretion Products of Shigella dysenteriae and Apoptotic Cell Death on Chick Embryo Muscle Tissue

The aim of this study was to evaluate the acute cell injury of excretion products present in culture filtrate from Shigella dysenteriae in both whole lower limb of chick embryo ex vivo and myoblasts cells developed in hanging-drop cultures in vitro. Three controls were defined: a) Tyrode's solu...

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Autores principales:	Alvarez,Marco, Urbina,Gidalia, Müller,Claudia, Perdomo,Lourdes
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2007
Materias:	Shigella dysenteriae Shiga toxin Chick embryo Muscle tissue Apoptosis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300024
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spelling	oai:scielo:S0717-950220070003000242007-10-30Excretion Products of Shigella dysenteriae and Apoptotic Cell Death on Chick Embryo Muscle TissueAlvarez,MarcoUrbina,GidaliaMüller,ClaudiaPerdomo,Lourdes Shigella dysenteriae Shiga toxin Chick embryo Muscle tissue Apoptosis The aim of this study was to evaluate the acute cell injury of excretion products present in culture filtrate from Shigella dysenteriae in both whole lower limb of chick embryo ex vivo and myoblasts cells developed in hanging-drop cultures in vitro. Three controls were defined: a) Tyrode's solution b) brain-heart broth infusion (CCC) and c) supernatant not toxigenic of E.coli 0157:H7. Shigella dysenteriae were culture for 24 hours and the excretion products were obtained after centrifugation of the culture. After lh of treatment, the morphologic changes in limbs treated with raw filtrate were evaluated through histopathological examination of sections stained with hematoxylin-eosin (H&E-stain) and Gomori's trichrome by image analysis techniques. Quantification of apoptotic cells was measured by an enzyme-linked immunoassay TUNEL. The morphological feature of apoptosis were evaluated in culture myoblasts. In contrast with controls, the longitudinal section on treated thigh of chick embryo limb-buds show atrophy muscle tissue, detachment of few fibers, 57,14% decrease in the number of cells, and loss of collagen substrate. Apoptotic index percent increase and mitotic index decrease in response to excretion products were observed, but were not significant. Membrane blebbing, vacuolation, small aggregates of chromatin around the nucleus and loss of cell adhesion were observed. Culture filtrate from Shigella dysenteriae produced cytotoxic effect on cell of muscle fibers with acute cell injuries suspected to be related to the apoptotic cell deathinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.25 n.3 20072007-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300024en10.4067/S0717-95022007000300024
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Shigella dysenteriae Shiga toxin Chick embryo Muscle tissue Apoptosis
spellingShingle	Shigella dysenteriae Shiga toxin Chick embryo Muscle tissue Apoptosis Alvarez,Marco Urbina,Gidalia Müller,Claudia Perdomo,Lourdes Excretion Products of Shigella dysenteriae and Apoptotic Cell Death on Chick Embryo Muscle Tissue
description	The aim of this study was to evaluate the acute cell injury of excretion products present in culture filtrate from Shigella dysenteriae in both whole lower limb of chick embryo ex vivo and myoblasts cells developed in hanging-drop cultures in vitro. Three controls were defined: a) Tyrode's solution b) brain-heart broth infusion (CCC) and c) supernatant not toxigenic of E.coli 0157:H7. Shigella dysenteriae were culture for 24 hours and the excretion products were obtained after centrifugation of the culture. After lh of treatment, the morphologic changes in limbs treated with raw filtrate were evaluated through histopathological examination of sections stained with hematoxylin-eosin (H&E-stain) and Gomori's trichrome by image analysis techniques. Quantification of apoptotic cells was measured by an enzyme-linked immunoassay TUNEL. The morphological feature of apoptosis were evaluated in culture myoblasts. In contrast with controls, the longitudinal section on treated thigh of chick embryo limb-buds show atrophy muscle tissue, detachment of few fibers, 57,14% decrease in the number of cells, and loss of collagen substrate. Apoptotic index percent increase and mitotic index decrease in response to excretion products were observed, but were not significant. Membrane blebbing, vacuolation, small aggregates of chromatin around the nucleus and loss of cell adhesion were observed. Culture filtrate from Shigella dysenteriae produced cytotoxic effect on cell of muscle fibers with acute cell injuries suspected to be related to the apoptotic cell death
author	Alvarez,Marco Urbina,Gidalia Müller,Claudia Perdomo,Lourdes
author_facet	Alvarez,Marco Urbina,Gidalia Müller,Claudia Perdomo,Lourdes
author_sort	Alvarez,Marco
title	Excretion Products of Shigella dysenteriae and Apoptotic Cell Death on Chick Embryo Muscle Tissue
title_short	Excretion Products of Shigella dysenteriae and Apoptotic Cell Death on Chick Embryo Muscle Tissue
title_full	Excretion Products of Shigella dysenteriae and Apoptotic Cell Death on Chick Embryo Muscle Tissue
title_fullStr	Excretion Products of Shigella dysenteriae and Apoptotic Cell Death on Chick Embryo Muscle Tissue
title_full_unstemmed	Excretion Products of Shigella dysenteriae and Apoptotic Cell Death on Chick Embryo Muscle Tissue
title_sort	excretion products of shigella dysenteriae and apoptotic cell death on chick embryo muscle tissue
publisher	Sociedad Chilena de Anatomía
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300024
work_keys_str_mv	AT alvarezmarco excretionproductsofshigelladysenteriaeandapoptoticcelldeathonchickembryomuscletissue AT urbinagidalia excretionproductsofshigelladysenteriaeandapoptoticcelldeathonchickembryomuscletissue AT mullerclaudia excretionproductsofshigelladysenteriaeandapoptoticcelldeathonchickembryomuscletissue AT perdomolourdes excretionproductsofshigelladysenteriaeandapoptoticcelldeathonchickembryomuscletissue
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Excretion Products of Shigella dysenteriae and Apoptotic Cell Death on Chick Embryo Muscle Tissue

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